Micropropagation of parsley through axillary shoot proliferation

Abstract

A micropropagation protocol of parsley, Petroselinum crispum (Mill.) Nyman (curled type) has been developed. Surface-sterilized axillary buds cultured on Murashige and Skoog medium supplemented with benzyladenine, kinetin, or thidiazuron developed axillary shoots (rosettes). Kinetin resulted in only a low proliferation rate. The concentrations of thidiazuron or benzyladenine that were optimal for shoot proliferation, resulted in shoots with a low capability to root. During the rooting treatment, these shoots showed wilting signs. Rooting was increased significantly by using a two-week inductive stage with 2.5 #M naphthaleneacetic acid directly followed by acclimatization. Two proliferation media (5/tM benzyladenine and 0.5 #M naphthaleneacetic acid or 5 #M kinetin and 2.5/~M naphthaleneacetic acid) resulted in moderate proliferation but produced shoots that were easyto-root. These media have been tested by repeated axillary proliferation on the same medium. The medium with 5 #M benzyladenine and 0.5 #M naphthaleneacetic acid was optimal.

Micropropagation of Heracleum candicans wall: A rare medicinal herb

“…1). Favorable effects of these growth regulator combinations on shoot proliferation response has also been reported in Foeniculum vulgare (Du Manoir et al, 1985), Petroselinum crispum (Vandemoortele et al, 1996), Cicer arietinum (Barna and Wakhlu, 1995), Hedeoma multiflorum (Koroch et al, 1997) and lsoplexis canariensis (Arrebola et al, 1997). The regenerated shoots were healthy and attained a height of 4.31 cm within 4 wk.…”

Micropropagation of Salvia brachyodon through nodal explants

“…Two weeks after the transfer of embryos on hormonefree ½ MS culture medium, seedlings developed in vitro. It was previously reported that low concentration of an auxin in combination with a cytokinin positively modifies the shoot induction frequency and their growth (Yuan et al 1994, Vandemoortele et al 1996, Singh and Sehgal 1999, Çöçü et al 2004. 1B), shoots were used in experiments to evaluate the effect of cytokinins on multiple shoot formation from nodal explants.…”

Propagation of Some Medicinal and Aromatic Plants in Turkey by Biotechnological Methods

“…- (Gopi & Ponmurugan, 2006). - (Vandermoortele, Billard, Boucaud, & Gaspar, 1996). - (Abbasi, Khan, Mahmood, Mushtaq, Chaudhary, & Khan, 2010).…”