Micropropagation of Physalis angulata L. and P. chenopodifolia Lam. (Solanaceae) via indirect organogenesis

Romo-Paz, Felipe de Jesús; Orozco-Flores, Jorge Daniel; Delgado-Aceves, Lourdes; Zamora-Natera, Juan Francisco; Salcedo-Pérez, Eduardo; Vargas-Ponce, Ofelia; Portillo, Liberato

doi:10.1007/s11627-023-10363-3

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…Uma solução para manter o cultivo de P. angulata viável sob longos períodos de tempo é o cultivo in-vitro, pois estudos recentes em diferentes espécies de plantas onde foram utilizadas técnicas de micropropagação mostraram que esta técnica pode ser uma solução para exploração comercial, propagação e conservação de espécies selecionadas, genótipos melhorados ou espécimes de elite (Afroz et al, 2010;Delgado-Aceves et al, 2021;Romo-Paz et al, 2023). Sendo isto, é importante a aplicação da técnica de organogênese que é uma importante técnica de cultura de tecidos in vitro que induz mudanças genéticas e aumenta a taxa de multiplicação de plantas para conservação (Su;Zhang, 2014).…”

Section: Resultsunclassified

Influência das embalagens nos períodos de armazenamento das sementes de Fisalis (Physalis angulata)

Mango,

Joaquim Júnior,

Sanó

et al. 2024

Rev. Car. Cien. Soc.

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Physalis angulata é uma planta de grande importância econômica devido a sua capacidade nutritiva e uso farmacêutico. Portanto, para fins comerciais a propagação desta espécie de deve a partir de sementes, o que deve requer atenção voltada a qualidade de sementes. Por isso, o presente estudo tem como objetivo, compreender o potencial das embalagens em manter a viabilidade das sementes em diferentes períodos de armazenamento. O estudo foi conduzido em delineamento inteiramente casualizado em esquema fatorial 3x4 (embalagem e período de armazenamento). Em cada embalagem foi colocado 1500 sementes e foram protegidas contra a luz com papel alumínio envolvendo as embalagens e embrulhados por um saco preto e armazenadas em ambiente de laboratório onde ficaram até o último período de armazenamento. Foram determinadas a umidade (U), primeira contagem (PC), a germinação (G), o Índice de velocidade de germinação (IVG), tempo médio de germinação (TMG) e envelhecimento acelerado (EA). Os dados foram submetidos à ANOVA pelo teste F. E em seguida as médias foram comparadas pelo teste de Tukey (p ≤ 0,05) para o fator embalagem e a análise de regressão para o fator armazenamento. Entretanto, não houve diferenças significativas para a interação dos dois fatores, porém, foi observado diferenças significativas quanto ao fator armazenamento isolado para todas as variáveis analisadas. O fator embalagem mostrou-se significativo apenas para variável umidade. A embalagem de vidro reduziu a umidade das sementes de Physalis angulata, sendo recomendada entre as demais utilizadas, visto que alto teor de umidade pode inviabilizar as sementes. Recomenda-se o uso das sementes de Physalis angulata logo nos primeiros dias de sua obtenção, sendo que a perda da viabilidade e de germinação destas sementes diminui ao longo do tempo de armazenamento.

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Section: Resultsunclassified

Influência das embalagens nos períodos de armazenamento das sementes de Fisalis (Physalis angulata)

Mango,

Joaquim Júnior,

Sanó

et al. 2024

Rev. Car. Cien. Soc.

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“…Furthermore, in vitro preservation is a source of tissues that are valuable for bioprospecting research [18], as well as the induction of mutagenesis for future applications. This biotechnological technique has several applications, including the study of physiological aspects [25], clonal propagation [26], the production of secondary metabolites [27], plant regeneration [28,29], and obtaining disease-free varieties [23,30,31].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

de la Luz,

Román,

Jorge

et al. 2024

Horticulturae

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the shoot propagation, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL−1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds and shoots and the shoot height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L−1 of BA and 0.1 mg L−1 of TDZ, as well as the MS base culture medium. The validation of the results of the first phase (0.1 mg L−1 of BA) was compared with the MS in an independent experiment against the control (n = 50 repetitions), obtaining a height of 52 mm, 1.36 shoots, and 9.22 buds, suggesting that this concentration is adequate for the purpose, surpassing the MS control (MS culture medium alone). Of the total volume of roots obtained with packed bud structure in the previous experimental sample, it was reduced to 14% (n = 50). The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L−1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. Callus induction in S. compositum was better when using the stem in a medium with 2.0 mg L−1 of 2,4-D with a value of 97.8% around the explant. The addition of 500 mg L−1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callus formation of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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“…Furthermore, in vitro preservation is a source of tissues that are valuable for bioprospecting research [18], as well as the induction of mutagenesis for future applications. This biotechnological technique has several applications, including the study of physiological aspects [25], clonal propagation [26], production of secondary metabolites [27] plant regeneration [28,29] and obtaining disease-free varieties [23,30,31].…”

Section: Introductionmentioning

confidence: 99%

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Luz,

Román,

Mar

et al. 2023

Preprint

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the multiplication of seedlings, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL-1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds, shoots, and seedling height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L-1 of BA and 0.1 mg L-1 of TDZ, as well as the MS base culture medium. With 0.1 mg L-1 of BA, 52-mm height, 1.36 shoots, and 9.22 buds were obtained, surpassing the MS control (MS culture medium alone); likewise, twice more roots (80%) and a 14% reduction in the bud structure were packed in 50 repetitions. The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L-1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. The results demonstrated that the induction of callogenesis in S. compositum has a better start from the stem in a medium with 2.0 mg L-1 of 2,4-D, whose value was 4.89 —equivalent to 97.8% callus formation around the explant. The addition of 500 mg L-1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callogenesis of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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Micropropagation of Physalis angulata L. and P. chenopodifolia Lam. (Solanaceae) via indirect organogenesis

Cited by 4 publications

References 49 publications

Influência das embalagens nos períodos de armazenamento das sementes de Fisalis (Physalis angulata)

Influência das embalagens nos períodos de armazenamento das sementes de Fisalis (Physalis angulata)

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

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