Micropropagation of pineapple (Ananas comosus L. Merr) MD2 variety through axillary bud proliferation

Abstract

The pineapple (Ananas comosus L. Merr) is an economically important bromeliad plant due to the organoleptic characteristics of its fruit, being the MD2 variety one of the most cultivated. Pineapple culture is limited by its inefficient sucker production. Micropropagation represents a way of producing pineapple plantlets. The aim of this study was to obtain a micropropagation protocol for A. comosus MD2 variety using the axillary bud proliferation technique. Axillary buds from suckers were put into MS media where 17 treatments were tested: benzylaminopurine (BA) in combination with indolebutyric acid (IBA) (0, 0.5, 1 mg/L) or indoleacetic acid (IAA) (0, 0.5, 1 mg/L); as well as BA (2 mg/L) with naphthaleneacetic acid (NAA) (5 mg/L); and kinetin (KIN) (2 mg/L). The presence or absence of silver nanoparticles (34 mg/L) was tested as well. The percentage of sprouting, number of shoots, and length were measured. Percentage of sprouting was higher (73.3%) when kinetin and silver were present. Highest shoot number (2.5±0.49) was observed on media containing BA 3 mg/L and AIA 0.5 mg/L. Also, organogenesis was observed on media with BA and ANA. Finally, acclimatization had a 100% success rate.

Effect of different concentrations of benzylaminopurine for the in vitro propagation of Vanilla tahitensis (J. W. Moore)

“…Por otro lado, Parismoreno et al (2021b) señalan que, aplicando la citocinina sintética Thidiazuron (TDZ) en concentración de 0,25 mg/L se obtuvo el mayor número de brotes, con un promedio de 3 brotes/explante de Phalaenopsis spp. Trabajos realizados por Salas-Valdivia et al (2023) encontraron que bencilaminopurina en concentraciones de 0; 1; y 3 mg/L en combinación con ácido indolbutírico (AIB) en concentraciones de 0; 0,5; y 1 mg/L o ácido indolacético (AIA) (0; 0,5; y 1 mg/L); así como BAP (2 mg/L) y ácido naftalenacético (ANA) (5 mg/L); y kinetina (KIN) (2 mg/L) en Ananas comosus L. Merr encontraron que el mayor número de brotes (2,5 ± 0,49) se dio en el tratamiento con BAP 3 mg/L + AIA 0,5 mg/L. Con relación al número de hojas, con la concentración de 2,0 mg/L de BAP se promovió el mayor número de hojas con promedios de 5 y 7 hojas a los 60 y 90 d, con relación a los demás tratamientos.…”