Micropropagation of pitaya (Hylocereus undatus Britton et rose)

Mohamed–Yasseen, Yasseen

doi:10.1079/ivp2002312

Cited by 39 publications

(25 citation statements)

References 6 publications

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“…In this study, addition of 6-BA along with auxin considerably increased morphogenetic response of red pitaya shoots as well as shoot number. Interestingly, the phytohormone combinations for the micropropagation of white pitaya (Hylocereus undatus) (Mohamed-Yasseen, 2002;Hua et al, 2015) were distinctly different from our findings.…”

Section: Discussioncontrasting

confidence: 99%

High-efficiency Micropropagation of Dormant Buds in Spine Base of Red Pitaya (Hylocereus polyrhizus) for Industrial Breeding

Qin¹,

Wang²,

He³

et al. 2017

IJAB

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By the systematical optimization on explants sterilizing, induction and multiplication of adventitious shoots, rooting and seedling transplanting, the high-efficient regeneration system and domestication transplanting technology for red pitaya (Hylocereus polyrhizus) were established using dormant buds in spine base as primary materials. Results showed that the optimal surface sterilization procedure of red pitaya explants (nodal segments with one spine base) was 0.2% mercuric chloride (HgCl 2 ) treatment for 7 min. The appropriate medium formulation for adventitious shoot induction and proliferation of sterilized red pitaya explants was Murashige and Skoog's medium (MS) supplemented with 5.5 mg/L 6-benzyladenine (6-BA) and 0.1mg/L α-naphthaleneacetic acid (NAA), and maximum average number (6.4) of adventitious shoots per explant and maximum average plantlet height (2.8 cm) were achieved within 30 days. An optimal formulation of half-strength MS medium (1/2 MS) added with 0.5 mg/L NAA and 0.3 mg/L indole-3-butyricacid (IBA) produced an average of 7.21 roots per explant with highest (92%) rooting response when inoculating 4 adventitious shoots per bottle. The tissue-cultured seedlings of red pitaya display a survival rate of up to 100% by optimized procedure of domestication and transplantation.

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Section: Discussioncontrasting

confidence: 99%

High-efficiency Micropropagation of Dormant Buds in Spine Base of Red Pitaya (Hylocereus polyrhizus) for Industrial Breeding

Qin¹,

Wang²,

He³

et al. 2017

IJAB

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“…very few starting materials. Currently, several researchers have established protocols for propagation of pitaya (Infante 1992;Drew and Azimi 2002;Mohamed-Yasseen 2002;Pelah et al 2002;Viñas et al 2012;Fan et al 2013). However, pitaya with diverse genetic backgrounds respond differently to the same culture medium, probably due to genetic conditioning (Drew and Azimi 2002;MohamedYasseen 2002;Fan et al 2013).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

A protocol for rapid in vitro propagation of genetically diverse pitaya

Hua

Chen

et al. 2014

Plant Cell Tiss Organ Cult

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An efficient propagation system of pitaya with diverse genetic background was established by using tender stem as explants. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for shoot proliferation. Zeatin (ZT), thidiazuron (TDZ), 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (6-BA) alone could induce multiple shoots. In that case, the most shoots per explant i.e. 4.6 (13.68 lM ZT), 4.8 (0.11 lM TDZ), 4.3 (17.76 lM 6-BA) and 3.2 (0.23 lM 2, 4-D) were obtained. The best PGRs combination was MS medium with 3.0 lM ZT and 0.5 lM IBA producing the most shoots per explant and the most vigorous shoots. Of the two varieties and six superior selections cultured on the best PGRs combination, more than 6.0 shoots per explant were obtained. No polymorphism was detected among in vitro-derived plantlets selected at random after 11 sub-cultures. Those results showed that MS media with 13.68 lM ZT and 2.46 lM IBA was suitable for shoot propagation of diverse pitaya varieties and selections. The protocol can be used for largescale propagation of pitaya to meet the demand of increasing commercial cultivation.

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“…from somatic tissues (Drew and Azimi 2002;Mohamed-Yasseen 2002), but both refer to H. undatus (white-fleshed pitahaya), a different species to that used in this study. Developing efficient micropropagation procedures for particular species generally requires detailed studies to define specific composition of mineral salts, plant growth regulators, and organic compounds in the culture medium.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of purple pitahaya (Hylocereus costaricensis [F.A.C. Weber] Britton & Rose) cv. Cebra

Viñas

Fernández-Brenes

Azofeifa

et al. 2012

In Vitro Cell.Dev.Biol.-Plant

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Limitations to large-scale propagation of purple pitahaya (Hylocereus costaricensis [F.A.C. Weber] Britton & Rose), a potential source of betalains for the food industry, can be overcome through utilization of in vitro culture technologies. In this work, successful in vitro propagation from areoles of adult purple pitahaya plants is reported. Factors affecting culture initiation, bud sprouting and growth, shoot multiplication, rooting, and acclimatization were studied. Best results for culture initiation were obtained from the central region of new joints by disinfection of large (5-7 cm in length) explants that were subsequently divided. Explants were sequentially treated with Extran® alkaline detergent for 10 min, followed by immersion in 70 % (v/v) ethanol for 15-30 s, a mixture of the fungicide Benomyl and the bactericide Agrymicin (2 gl −1 each) for 30 min, and disinfection in sodium hypochlorite (1.0 %w/v) for 15 min. Culture of sectioned individual areoles, without removing thorns, on Murashige and Skoog medium supplemented with 15 or 30 μM N 6 -benzylaminopurine (BAP) for 3 mo with monthly subcultures, followed by transfer to the same medium with reduced BAP (0-2 μM), induced bud sprouting in over 80 % of explants, adequate growth of the shoots, with production of lateral shoots, and spontaneous rooting within 160 d. These plants were successfully acclimatized in vermiculite and peat moss (1:1), or perlite and peat moss (2:1) in the greenhouse, with over 90 % survival rate. One hundred percent of the in vitroderived plants were successfully transferred to the field. Furthermore, these plants showed higher survival rates, larger height and increase in stem diameter than equivalent plants from the same genotype, derived from stem segments (the common clonal propagation system utilized for this species) that were simultaneously planted.

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Micropropagation of pitaya (Hylocereus undatus Britton et rose)

Cited by 39 publications

References 6 publications

High-efficiency Micropropagation of Dormant Buds in Spine Base of Red Pitaya (Hylocereus polyrhizus) for Industrial Breeding

High-efficiency Micropropagation of Dormant Buds in Spine Base of Red Pitaya (Hylocereus polyrhizus) for Industrial Breeding

A protocol for rapid in vitro propagation of genetically diverse pitaya

In vitro propagation of purple pitahaya (Hylocereus costaricensis [F.A.C. Weber] Britton & Rose) cv. Cebra

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