Micropropagation of Pyrus calleryana Dcn. from seedlings

Berardi, G.; Infante, Rodrigo; Neri, Davide

doi:10.1016/0304-4238(93)90146-h

Cited by 18 publications

(11 citation statements)

References 12 publications

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“…Both in vitro and ex vitro rooting of micro-propagated shoots of P. elaeagrifolia were only accomplished by auxin treatments. Such results were found in some other Pyrus species and woody plants ( Bhojwani et al, 1984 ; Shen and Mullins, 1984 ; Baviera et al, 1989 ; McClelland et al, 1990 ; Berardi et al, 1991 , 1993 ; Dolcet-Sanjuan et al, 1991 ; Moretti et al, 1991 ; Rodriguez et al, 1991 ; Rossi et al, 1991 ; Al-Maarri et al, 1994 ; Reed, 1995 ; Yeo and Reed, 1995 ; Shibli et al, 1997 ; Iglesias et al, 2004 ; Barros et al, 2005 ). In the current study, IBA at low concentration (5 μM) with dark treatment (10 days) stimulated in vitro root formation in micro-cuttings of P. elaeagrifolia .…”

Section: Discussionsupporting

confidence: 53%

“…Similarly, especially for recalcitrant Pyrus genotypes, treatment with 10 μM IBA during 1 or 3 weeks in darkness was suggested ( Reed, 1995 ; Yeo and Reed, 1995 ), but IBA concentrations lower than 10 μM were not tested in the studies. Berardi et al (1993) found that in vitro rooting in P. calleryana was promoted by auxins, mainly by NAA (2.7 μM). Shibli et al (1997) reported that IBA, IAA, and NAA induced in vitro rooting of P. syrica and a maximum of 72% rooting was achieved at 17 μM IAA.…”

Section: Discussionmentioning

confidence: 97%

“…In vitro shoot proliferation of P. elaeagrifolia was affected by BA concentrations (4.5 and 9.0 μM) in both initial cultures and subcultures. It was reported that BA concentrations of 3.3 to 20 μM produced successful multiplication of P. communis ( Singha, 1982 ; Shen and Mullins, 1984 ; Baviera et al, 1989 ; Dolcet-Sanjuan et al, 1990 ; Moretti et al, 1991 ; Rodriguez et al, 1991 ; Yeo and Reed, 1995 ; Iglesias et al, 2004 ), P. calleryana ( Dolcet-Sanjuan et al, 1990 ; Rossi et al, 1991 ; Berardi et al, 1993 ; Yeo and Reed, 1995 ), P. amygdaliformis ( Dolcet-Sanjuan et al, 1990 ), P. betulifolia ( Dolcet-Sanjuan et al, 1990 ; Yeo and Reed, 1995 ), and P. syrica ( Shibli et al, 1997 ). Generally, researchers used BA alone in micro-propagation of Pyrus species and very little information is available about the effect of auxins on multiplication in this genus.…”

Section: Discussionmentioning

confidence: 99%

“…Axillary bud multiplication is a widely used means of mass propagation of plants that are genotypically and phenotypically the same as the original plant from which they were produced ( Evans, 1990 ). Results on micropropagation of P. communis ( Cheng, 1979 ; Shen and Mullins, 1984 ; Rodriguez et al, 1991 ; Al-Maarri et al, 1994 ; Iglesias et al, 2004 ), P. calleryana ( Berardi et al, 1993 ), P. calleryana , P. betulaefolia ( Yeo and Reed, 1995 ), and P. syriaca ( Shibli et al, 1997 ) have been reported by several researchers ( Chevreau and Bell, 2005 ). However, micropropagation of P. elaeagrifolia has not been studied.…”

Section: Introductionmentioning

confidence: 88%

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In vitro shoot proliferation and in vitro and ex vitro root formation of Pyrus elaeagrifolia Pallas

Aygün

Dumanoğlu

2015

Front. Plant Sci.

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Shoot-tip cultures of Pyrus elaeagrifolia Pallas, an important gene source for drought and chlorosis resistance in pear rootstock breeding, were established from a wild mature tree originated from seed. Murashige and Skoog basal medium supplemented with different concentrations of benzyladenine (BA) singly or in combination with auxin was used in the study. In the initial culture, the highest percentages (>80%) of shoot proliferation were obtained in the mediums supplemented with 9.0 μM BA and 0.5 μM indole-3-acetic acid. In the subcultures, the highest shoot proliferation rates were obtained in the medium containing 4.5 and 9.0 μM BA. The shoot proliferation rates ranged from 91.1 ± 2.4 to 96.4 ± 2.0% in the second subculture and from 76.7 ± 7.8 to 89.4 ± 3.3% in the third subculture. In the second subculture, the shoots grown on 9.0 μM BA without auxin produced the best proliferation (10.6 ± 1.6). For the in vitro rooting experiments, the highest rooting rate (54.2 ± 10.4%), root length (10.5 ± 2.4 mm), and root number (2.5 ± 0.6) were obtained from 10 days dark treatment on the medium containing half strength of macronutrients supplemented with 5 μM indole-3-butyric acid (IBA). For the ex vitro rooting experiments, shoot rooting was significantly influenced by 10 mM IBA applied as quick-dip method. The percentage of rooting was 55 ± 9.6% and root number was 1.8 ± 0.3 at this concentration.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

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In vitro shoot proliferation and in vitro and ex vitro root formation of Pyrus elaeagrifolia Pallas

Aygün

Dumanoğlu

2015

Front. Plant Sci.

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“…The beneficial effects of adding exogenous antiauxin to the medium are likely a result of altering the balance of auxins and cytokinins, thereby preventing the occurrence of apical dominance. Studies with a number of taxonomically diverse species have indicated that medium with a cytokinin such as BA is not able to maintain the multiplication rate over multiple subcultures (Berardi et al 1993;Singh and Syamal 2000) and that the inhibition of auxin activity can have a beneficial effect on long-term shoot proliferation.…”

Section: Discussionmentioning

confidence: 99%

In vitro conservation of American elm (Ulmus americana): potential role of auxin metabolism in sustained plant proliferation

ShuklaMukund

Alan

et al. 2012

Can. J. For. Res.

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An efficient procedure for the conservation of mature American elm ( Ulmus americana L.) trees that have survived the epidemics of Dutch elm disease and are potential sources of disease resistance is reported. The model utilizes in vitro proliferation of fresh and dormant buds from mature trees for cloning nearly 100 year old American elm trees. The key factors that influenced sustained growth and multiplication included optimization of culture process and auxin metabolism in the source tissue. Blocking the action of endogenous auxins through the addition of antiauxin in the proliferation medium was crucial for high multiplication rate and optimum shoot development. Addition of antiauxin also mitigated the decline in productivity observed with multiple subcultures, which will enable long-term conservation of selected germplasm. The most effective medium for long-term proliferation contained 5.0 µmol/L p-chlorophenoxyisobutyric acid with 2.2 µmol/L benzylaminopurine and 0.29 µmol/L gibberellic acid. Medium with 2.5 µmol/L indole-3-butyric acid was the best for rooting microshoots (89%). Rooted plantlets were readily acclimatized to the greenhouse environment with a 90% survival rate. The strategy developed for American elm will aid in increasing multiplication of resistant clones, facilitate long-term conservation of elite genotypes, and also provide an approach to improve conservation of other endangered tree species.

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