Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation

Echeverrigaray, Sérgio; Carrer, Rosane Postingher; Andrade, Luciana Bavaresco

doi:10.1590/s1516-89132010000400018

Cited by 23 publications

(13 citation statements)

References 14 publications

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“…BAP was the most effective cytokinin for the multiplication of shoots in C. collinus. This result was in accordance with the findings of Portulaca grandiflora (Jain and Bashir, 2010), Thymus satureioides (Aicha et al, 2013) and Salvia guaranitica (Echeverrigaray et al, 2010). On the other hand when the cytokinin level was increased there was a reduction and stunted shoots.…”

Section: Discussionsupporting

confidence: 91%

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Elangomathavan¹,

S²,

Hariharan³

et al. 2017

Int. J. Adv. Res. Biol. Sci

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In this study an effective plant regeneration protocol for multiple shoot bud induction was optimized for the micropropagation of plantlets through indirect organogenesis from leaf explants of C. collinus. The young leaves were cultured on Murashige and Skoog (MS) medium with Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin (KIN), 2,4-Dichlorophenoxyacetic acid (2,4-D) and 1-Naphthaleneacetic acid (NAA) at 0.5-3.0 mg/l for callus induction. The induced calli was excised and subcultured on MS medium supplemented with BAP, KIN and TDZ (0.5-4.0 mg/l) in combination with NAA (0.5 mg/l) to find out the optimum hormone concentration for shoot bud induction. For rooting, half strength MS medium with various concentrations (0.5-3.0 mg/l) of Indole 3-acetic acid (IAA), Indole 3-butyric acid (IBA) and NAA was used. Initially, a creamy white callus was formed from the cut ends of leaf explants and gradually these mass of unorganised cells transformed to light green regenerable callus. The maximum callus induction (100%) was observed in MS medium with TDZ at 1.0 mg/l concentration. The highest frequency of organogenesis was observed in the combination of BAP (1.0 mg/l) and NAA (0.5 mg/l) with a mean number of 12.1±1.1 shoots followed by TDZ (1.0 mg/l) and NAA (0.5 mg/l) with 10.2±1.0 shoots per explants. The healthy shoots were rooted best on half strength MS medium supplemented with 0.5mg/l IAA. The well developed plantlets with healthy roots were successfully acclimatized. The development of thriving protocol for the callus induction and regeneration would make possible for the mass multiplication and in vitro production of plantlets with somaclonal variations.

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Section: Discussionsupporting

confidence: 91%

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Elangomathavan¹,

S²,

Hariharan³

et al. 2017

Int. J. Adv. Res. Biol. Sci

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“…Earlier reports showed the efficiency of BAP in production of multiple shoots on Portulaca grandiflora [44], Thymus satureioides [45], Origanum sipyleum [46], and Salvia guaranitica [47]. However, increased concentration of BAP and KIN beyond the optimum level resulted in the reduction of shoot per explants.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

<i>In Vitro</i> Callus Induction and Shoot Regeneration from Leaf Explants of <i>Glinus lotoides</i> (L.)—An Important Medicinal Plant

Teshome¹,

Feyissa²

2015

AJPS

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G. lotoides L. is a threatened plant that is frequently harvested for medicinal purpose. However, its distribution in the world is limited because of short period of seed viability and poor seed germination. The objective of this study was to develop in vitro propagation protocol for G. lotoides through callus induction. For callus induction, different concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid), NAA (α-naphthalene acetic acid) and BAP (6-benzyl amino purine) were used. Seeds were sown on growth regulator-free MS medium and shoots from the in vitro germinated seedlings were excised and cultured on MS medium containing 0.5 mg/l BAP. Young leaves from these shoots were used as explant for callus induction and shoot regeneration. Maximum callus induction (100%) was observed on medium containing 2,4-D (0.5, 2.0, 3.5 mg/l) or NAA (2.0, 2.5 mg/l) in combination with 0.5 mg/l BAP. However, 2,4-D was the best in overall callus induction. The highest regeneration (20%) frequency was achieved on the medium containing 0.5 mg/l BAP. Highest number of shoot (2.83 ± 1.22) and shoot length (2.16 ± 0.87 cm) per explant were obtained in the presence of 0.25 mg/l BAP + 0.5 mg/l KIN (Kinetin). In shoot multiplication media, maximum mean (6.43 ± 0.87) of shoot was observed on MS medium containing 0.5 mg/l BAP. The best shoot length (1.70 ± 0.14 cm) was recorded on control medium. The highest (95%), maximum root number (14.10 ± 1.47) and root length (1.01 ± 0.10 cm) were obtained on a medium supplemented with 1.5 mg/l Indole-3-butyric acid (IBA). All the plants (100%) were survived after acclimatization in greenhouse. The present study can be useful for callus induction and indirect shoot regeneration form G. lotoides.

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“…This choice was not accidental, as it has been found that cytokinins possessing a heterocyclic system in their structure gave better results in the multiplication of Salvia species. Media containing 0.5 mg/L benzylaminopurine, were found to yield a multiplication atio for Salvia guaranitica 2.5 times that of a culture treated with the same TDZ (thidiazuron) content [8]. BAP was also more effective than thidiazuron in the proliferation of Salvia brachyodon [9], Salvia nemorosa [10], and Salvia fruticosa [11].…”

Section: Introductionmentioning

confidence: 99%

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

et al. 2020

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The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.

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Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation

Cited by 23 publications

References 14 publications

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

<i>In Vitro</i> Callus Induction and Shoot Regeneration from Leaf Explants of <i>Glinus lotoides</i> (L.)—An Important Medicinal Plant

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

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Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation

Cited by 23 publications

References 14 publications

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

Indirect shoot organogenesis from leaf explants of threatened medicinal plant Cleistanthus collinus

&lt;i&gt;In Vitro&lt;/i&gt; Callus Induction and Shoot Regeneration from Leaf Explants of &lt;i&gt;Glinus lotoides&lt;/i&gt; (L.)—An Important Medicinal Plant

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

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<i>In Vitro</i> Callus Induction and Shoot Regeneration from Leaf Explants of <i>Glinus lotoides</i> (L.)—An Important Medicinal Plant