Micropropagation of Sicilian cultivars with an aim to preserve genetic diversity in hazelnut (Corylus avellana L.)

Cryopreservation of Hazelnut (Corylus avellana L.) Axillary Buds from In Vitro Shoots Using the Droplet Vitrification Method

“…Shoots originated from the axillary buds cryopreserved using PVS3 for a duration of 60 min were multiplied for five subcultures. Then, 30 of these shoots and 30 from standard cultural conditions, as a control, were induced to root, according to Sgueglia et al [41].…”

“…Shoots originated from the axillary buds cryopreserved using PVS3 for a duration of 60 min were multiplied for five subcultures. Then, 30 of these shoots and 30 from standard cultural conditions, as a control, were induced to root, according to Sgueglia et al [41]. Briefly, shoots were transferred to a rooting medium, containing half strength MS macro salts, full MS micro salts and organics [55], 20 gL −1 sucrose, 5.6 gL −1 agar (B&V, Parma, Italy), and 17.6 µM IBA, in standard cultural conditions.…”

“…The conservation of local cultivars is essential for breeders and stakeholders [33,34] and their micropropagation is a suitable tool for the production of true-to-type and diseasefree plants. Micropropagation of hazelnut cultivars has been described in several studies [35][36][37][38][39][40] and it is commonly used in the USA, Chile, and Italy as a tool for propagation and conservation of genetic resources [33,41]. In vitro cultures also represent the base for cryopreservation and rapid clonal multiplication offering a possibility for the reintroduction of ancient cultivars.…”

Особливості технології клонального розмноження фундука шляхом живцювання в умовах in vitro та шляхи підвищення її ефективності

“…Водночас, це найбільш надійний спосіб отримання генотипічно однорідного зі стабільним геномом матеріалу, який відповідає потребам селекції і меті збереження генофонду цінних сортів. Особливо це важливо для підтримки колекцій чистих ліній ex та in situ [44,57].…”

Is It Possible to Produce Certified Hazelnut Plant Material in Sicily? Identification and Recovery of Nebrodi Genetic Resources, in vitro Establishment, and Innovative Sanitation Technique From Apple Mosaic Virus

“…The traditional nursery propagation method of hazelnuts is based on the use of suckers from mound layering, a time-consuming technique with low efficiency at the commercial level ( Mardani et al, 2020 ). Therefore, the micropropagation method is not only considered as an appropriate alternative for producing high quality, disease-free, and true-to-type hazelnut plants, independent from the season and environmental conditions ( Debergh et al, 1991 ), but also it represents a valuable tool for plant biodiversity conservation ( Sgueglia et al, 2018 , 2019 ). However, hazelnut genotypes commonly show a difficult in vitro establishment due to surface sterilization failure, strictly related to the very common problem of endogenous contamination, showing even after weeks or months after culture initiation, besides to the lack of young plant material ( Hand et al, 2016 ; Javed et al, 2017 ).…”