Micropropagation of Syzygium densiflorum Wall. ex Wight &amp; arn.: An endemic and endangered semi-evergreen tree species of the Western Ghats, India

Ramasubbu, Raju; Divya, C.

doi:10.1016/j.tfp.2020.100037

Cited by 5 publications

(4 citation statements)

References 47 publications

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“…1). These results are in agreement with the findings of other investigations that have described the promotive effects of cytokinins in combination with auxins on shoot proliferation rate of other plant species (Kaliamoorthy et al, 2008;Alawaadh et al, 2020;Raju and Divya, 2020).…”

Section: Discussionsupporting

confidence: 93%

“…Similar to results of our study, the principal role of cytokinins in in vitro organogenesis processes including shoot proliferation has been widely reported (Veraplakorn, 2016;Hosseinabadi et al, 2020;Kumar and Giridhar, 2020;Nithya and Kamalam, 2021;Farooq et al, 2022). In fact, cytokinins play a crucial role in stimulating cell division and synthesis of various macromolecules including RNA, proteins and enzymes and they are notably used in in vitro cultures to suppress apical dominance and promote axillary shoot proliferation (Gomes et al, 2010;Mohamad et al., 2022;Farooq et al, 2021;Fawzia et al, 2018;Raju and Divya, 2020). However, optimal rate of shoot proliferation largely depends on the concentration of cytokinins used and it has been well established that different types of cytokinins have varying efficiencies in inducing shoot multiplication, and their effectiveness is dose-dependent (Jun-jie et al, 2017;Bayraktar et al, 2020;Jagiełło-Kubiec et al, 2021).…”

Section: Discussionsupporting

confidence: 88%

“…This method has gained significant commercial importance as it allows for the large-scale propagation of desired plants, thus playing a crucial role in the global ornamental industry. In fact, in vitro cultures offer several advantages over traditional methods of plant propagation, including the relatively rapid mass production of plant species and a reduced cost of production (Nakano et al, 2004;Al-Aizari et al, 2020;Raju and Divya, 2020;Showkat Bhat et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

Akramian,

Khaleghi,

Salehi Arjmand

2024

JGPP

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

Akramian,

Khaleghi,

Salehi Arjmand

2024

JGPP

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“…In our study concur with earlier studies conducted in several other woody tree species 29,30 . Frequently, cotyledonary node are routinely used tissue explants in tree species Syzygium densiflorum 31 was reported.…”

Section: Cotyledonary Nodal Culturesmentioning

confidence: 99%

In vitro propagation of threatened therapeutic tree Buchanania lanzan Spreng (Anacardiaceae)

Kannan

Kumar

Saralla

2022

J. Drug Delivery Ther.

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Embryo rescue refers to an in vitro technology have been used for propagating plants under in vitro from poorly developed embryos by avoiding embryo abortion. The aim of the present study was to in vitro regeneration of a medicinally wild tree species Buchanania lanzan through embryo rescue method. Isolated immature poorly developed embryo dissected out from de-coded seeds collected from 15 years old trees were cultured an Woody Plant Medium (WPM) was used to test in vitro germination. 100% embryo germination were recorded when the embryos inoculated on the medium supplemented with 1.5 mg/L-1 6-benzylaminopurine (BAP) and 50 mg/L-1 adenine sulphate (AS). Further, a week-old seedling derived explants viz embryo axis and cotyledonary node were selected to study in vitro clonal propagation. On medium augmented with 1.5 mg/L-1 6-benzylaminopurine (BAP) showed maximum mean shoots numbers (4.2±1.80) with 4.0±0.98 cm shoot height were achieved in cotyledonary node. Compare to Kinetin (Kn) BAP was to be better in shooting response and multiple shoot induction. Healthy regenerated shoots was separated from multiple shoots cluster and transferred to a rooting medium containing half strength WP medium fortified with Indole-3-butyric acid (IBA). IBA at 0.5 mg/L-1 showed 90% rooting response and maximum mean roots (4.9±0.98) per shootlet was obtained. The rooted in vitro plantlets were transferred to polythene bags containing autoclaved humus, red soil and farmyard manure (1:1:1 ratio) for acclimation at 25 ± 2 ᵒC. Later, these plantlets were gradually transferred to field condition. About 70% survival rate was recorded after three months period. The protocol developed in the present study was to restore the viable embryos from poorly developed immature embryos and rapid multiplication can help to ex situ conservation of this threatened tree B. lanzan. Keywords: Woody plant medium, In vitro seedlings, 6-benzylaminopurine, Adenine sulphate, Indole-3-butyric acid

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Conservation Attempts of Woody Medicinal Plants of India by Biotechnological Tools

Jeyaram,

Prasannan,

Pandian

et al. 2023

Bioprospecting of Tropical Medicinal Plants

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Micropropagation of Syzygium densiflorum Wall. ex Wight & arn.: An endemic and endangered semi-evergreen tree species of the Western Ghats, India

Cited by 5 publications

References 47 publications

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

In vitro propagation of threatened therapeutic tree Buchanania lanzan Spreng (Anacardiaceae)

Conservation Attempts of Woody Medicinal Plants of India by Biotechnological Tools

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