Micropropagation of Ten Genotypes of Sugarcane Mutants on Media Containing BA and Thidiazuron

Abstract

Mutation induction in sugarcane by gamma irradiation has produced 10 mutant clones. Each mutant clone needs to be reproduced before further release. Multiplication of mutant clones is done in-vitro to produce true-to-type mutant sugar cane seeds. Four in-vitro media containing BA (Benzylaminopurine) and Thidiazuron were designed to obtain the right media for each sugarcane mutant. The medium used was basic Murashige Skoog (MS) medium containing 100 mg/l PVP and 3% sucrose, with the addition of BA (1 and 3 mg/l) and thidiazuron (0 and 0.5 mg/l). The explants used were shoots from the ten genotypes of the sugarcane mutants, measuring ±0.5 cm. The study used a completely randomized design with observational variables: shoot height, number of shoots, number of roots, visual shoots and roots. The results showed that growth media containing 3 mg/l BA and 0.5 mg/l thidiazuron was the best medium for multiplication of mutant sugarcane. Growth regulator auxin NAA was more effective in inducing the formation of sugarcane roots. Clones K10 and K8 had the best shoot multiplication ability compared to other mutant clones.