Micropropagation of Thapsia garganica—a medicinal plant

Makunga, Nokwanda P.; Jäger, Anna K.; Staden, J. Van

doi:10.1007/s00299-003-0623-8

Cited by 50 publications

(48 citation statements)

References 13 publications

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“…The produced plantlets could be directly acclimatized without passing through a rooting phase. Likewise, shoot production and rooting in one-phase was also observed for Thapsia garganica (Makunga et al 2003), Pinguicula lusitanica (Gonçalves et al 2008) and Crithmum maritimum (Grigoriadou and Maloupa 2008).…”

supporting

confidence: 54%

Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis

Gonçalves¹,

Martins²,

Romano³

2009

Biologia plant.

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Plantago algarbiensis and P. almogravensis are endemic Al tolerant species from the Western-centre of the Algarve region (South of Portugal) and Portuguese Southwest coast, respectively, which are in risk of global extinction. The aim of this work was to establish an efficient protocol to in vitro propagate these species using shoots obtained from in vitro germinated seeds. The best results in terms of multiplication response were afforded in Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (8.5 and 9.2 shoots per explant in P. algarbiensis and P. almogravensis, respectively). Shoots of both species showed a great rooting capacity (100 and 80 % for P. algarbiensis and P. almogravensis, respectively) that was not significantly influenced by the concentration of MS macronutrients or auxins. Plants were acclimatized to ex vitro conditions, exhibited normal development (survival rate of 95 and 80 % in P. algarbiensis and P. almogravensis, respectively), and were successfully reintroduced in their natural habitat.

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supporting

confidence: 54%

Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis

Gonçalves¹,

Martins²,

Romano³

2009

Biologia plant.

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“…Shoot proliferation decreased significantly under higher concentrations of BA, or when no IAA was present. Higher concentrations of BA (whether applied alone, or in combination with auxin) are known to decrease the number of new microshoots per explant in other for 12 months Apiaceae such as Thapsia garganica (Makunga et al 2003), E. foetidum (Gayatri et al 2006), Crithmum maritimum (Grigoriadou and Maloupa 2008), Arracacia xanthorrhiza (Sliva et al 2010), and Anethum graveolens (Jana and Shekhawat 2012). In contrast, the efficiency of shoot proliferation in Centella asiatica, and Vanasushava pedata was seen to increase in concentrations of up to 5.0 mg L -1 BA (Tiwari et al 2000;Karuppusamy et al 2006).…”

Section: Shoot Induction and Proliferationmentioning

confidence: 99%

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Thiem

Kikowska

Krawczyk³

et al. 2013

Plant Cell Tiss Organ Cult

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A protocol for in vitro production of genetically uniform populations of the medicinal plant Eryngium planum, rich in selected phenolic acids, has been established. Shoot-tips were collected from axenic seedlings and grown on a Murashige and Skoog basal medium supplemented with 6-Benzyladenine (BA) and Indole-3-acetic acid (IAA). The highest shoot proliferation efficiency (17 shoots per explant) was obtained when 1.0 mg L -1 BA and 0.1 mg L -1 were added. Proliferating shoots were rooted and transferred to soil (89 % frequency of survival). Flow cytometric analysis of intact (field-grown) and microrpropagated plants revealed that all plants were uniform in genome size and had similar DNA contents. Thin-layer chromatography (TLC) analysis indicated that multiple shoots and roots from in vitro-derived plants produced high amounts of phenolic acids, primarily of rosmarinic acid (RA). Levels of phenolic acids in in vitroderived plants were similar to those of intact plants. Furthermore, high-performance liquid chromatography revealed that root cultures in liquid medium accumulated substantial levels of RA. Thus, rapid establishment of in vitro-grown organ cultures of E. planum can also serve as reliable sources for bioactive compounds.

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“…Root development with an intermediary callus indicated that at that roots and shoots were not well connected with vascular tissue and that plants are insuffi ciently supplied with nutrients (George 1993). Successful rooting in other members of the Apiaceae family was also achieved with 1-5 μM IBA (Tavares et al 2010a, Das et al 2008, Jana and Shekhawat 2011, Coste et al 2012, Jaheduzzaman et al 2012, and with higher concentrations of IBA (Hossain et al 2000, Sharma and Wakhlu 2001, Banerjee et al 1999, Tavares et al 2009-2010, with NAA or IAA (Karuppusamy et al 2007, Jaheduzzaman et al 2012) and only rarely without growth regulators (Makunga et al 2003).…”

Section: Root Initiationmentioning

confidence: 99%

“…The seeds in our experimental system germinated only after cold treatment and did not germinate at all in growth chamber conditions. The reason for this late and low germination could be dormancy, which is not unusual for seeds of the Apiaceae family (Hendrawati et al 2012, Baskin andBaskin 2014), or possible loss of viability (Makunga et al 2003, Mondoni et al 2011. Sterilization only slowed the rate of early germination and slightly infl uences the fi nal germination of cold treated seeds.…”

Section: In Vitro Seed Germination and Initiation Of The Culturementioning

confidence: 99%

Micropropagation of the Narrow Endemic Hladnikia pastinacifolia (Apiaceae)

Ambrožič-Dolinšek¹,

Ciringer²,

Kaligarič³

2016

Acta Botanica Croatica

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-The monotypic Hladnikia pastinacifolia Rchb. is a narrow endemic species, with an extremely small distribution area in Slovenia, prone to any kind of threat that could lead to species extinction. Tissue culture techniques are proposed as a conservation measure for rapid propagation and ex-situ conservation. Tissue culture was initiated from seeds and juvenile plants obtained from natural sites on a solid Murashige and Skoog (MS) medium, with and without growth regulators. We tested various combinations and concentrations of growth regulators, and the best proliferation of axillary shoots, on average 14, was obtained on MS medium with 5 μM BAP and 3 μM IBA and 3% sucrose. Rooting was achieved after transferral of the shoots to an MS medium with 2 μM IBA and 3% sucrose. The rooted plants were acclimatized on a mixture of limestone sand, potting soil and vermiculite in a ratio of 10:2:2, with pH in the range of 7.5-8.0. In vitro propagation methods provide an important opportunity for the propagation and preservation of H. pastinacifolia by rapidly increasing the number of plants, without disturbing the wild population.

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Micropropagation of Thapsia garganica—a medicinal plant

Cited by 50 publications

References 13 publications

Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis

Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Micropropagation of the Narrow Endemic Hladnikia pastinacifolia (Apiaceae)

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