Micropropagation of the bromeliad Guzmania ‘Hilda’ via organogenesis and the effect of α-naphthaleneacetic acid on plantlet elongation

Huang, Ping-Lung; Liu, Zin-Huang; Chang, Min-Lang; Liao, Li‐Jen

doi:10.1016/j.scienta.2011.08.035

Cited by 7 publications

(7 citation statements)

References 29 publications

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“…Cam) Mez (Silveira et al 2009), Guzmania 'Hilda' (Huang et al 2011a), Dyckia distachya Hassler (Pompelli & Gerra 2005), Vriesea gigantea Gaudich. and Vriesea philippocoburgii Wawra (Droste et al 2005).…”

Section: Key-wordsmentioning

confidence: 99%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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The tissue culture can contribute to the propagation of several economic species, such as the bromeliads. This research aimed at evaluating the auxins type and concentration in the in vitro and ex vitro rhizogenesis of Neoregelia concentrica bromeliad. N. concentrica shoots were induced in a growth medium with 15.0 µM of 6-benzylaminopurine, for 80 days, followed by sub-cultivation in phytoregulator-free medium, for 45 days. In the in vitro rhizogenesis, the shoots grew in a medium supplemented with indole-3-butyric acid (IBA) or naphthalene-acetic acid (NAA), at the concentrations of 0.0 µM, 1.0 µM, 2.0 µM, 3.0 µM and 4.0 µM. In the ex vitro rhizogenesis, the bases of shoots were immersed, for 60 minutes, in IBA or NAA solutions, at the concentrations of 0.0 µM, 5.0 µM, 10.0 µM and 15.0 µM. After immersion, the shoots were planted in plastic trays with vermiculite. At the end of each rhizogenesis method, the phytotechnical parameters analysis was carried out. For the in vitro rhizogenesis, a higher number of roots were observed when the shoots were cultivated in concentrations higher than 1.0 µM of NAA, when compared to the IBA. However, the rooting rate differed only at 30 days after the in vitro growth, with a higher root induction in the shoots grown with NAA. At 60 days, the rooting rate was higher than 90% and statistically similar in all treatments. In the ex vitro rhizogenesis, a better formation of the rooting system was observed when 5.0 µM of IBA was applied, with higher rooting averages and number of roots.

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Section: Key-wordsmentioning

confidence: 99%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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“…One strategy for inducing NCs in bromeliads primarily uses leaf bases [ 3 6 7 ], although induction from nodal segments [ 4 ] and seeds has also been reported [ 12 ]. Moreover, similar structures were induced from floral segments [ 13 14 ]. Dal Vesco and Guerra [ 3 ] established an in vitro regeneration protocol based on the induction, multiplication and development of NCs of V. reitzii .…”

Section: Introductionmentioning

confidence: 99%

Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Corredor-Prado

Schmidt

Guerra

et al. 2015

J Microsc Ultrastruct

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Micropropagation via induction, multiplication and development of nodular cultures (NCs) is an efficient regeneration system for Bromeliaceae, a family of endangered monocot plants with ornamental value. Therefore, the present work aimed to induce NCs from seeds and leaf explants of Vriesea in order to characterize the morphological and histochemical aspects of induction and formation of these cultures. Seeds of Vriesea friburgensis var. paludosa were sterilized and inoculated into liquid culture media supplemented with different concentrations and combinations of growth regulators. Leaf explants of Vriesea reitzii were inoculated into medium supplemented with 4 μM α-naphthalene acetic acid (NAA) and 2 μM 6-benzylaminopurine (BAP). The addition of NAA (4 μM) in the culture medium used for seeds led to an induction rate of 72% in NCs. First, the embryo began to germinate, and afterwards, nodular structures started to form. While NCs formed from seeds is associated with root and shoot meristems, the formation of NCs from leaf explants involves the intercalary meristem. Meristematic cells generate an appropriate response in the induction medium, producing NCs by the proliferation of small cells with meristematic characteristics and large vacuolated cells. These results provide a better understanding of morphogenetic responses in bromeliads and, hence, the opportunity to develop optimized micropropagation protocols.Abbreviations: BAP, 6-benzylaminopurine; 2-iP, N6 (2-isopentyl) adenine; CBB, Coomassie Brilliant Blue; CLSM, confocal laser scanning microscopy; MSB, MS basal medium; NAA, α-Naphthalene acetic acid; NCs, nodular cultures; PAS, Periodic Acid-Schiff; SEM, scanning electron microscopy; TDZ, N-phenyl-N’-1,2,3-thidiazol-5-ylurea; TB-O, Toluidine Blue O; TEM, Transmission electron microscopy.

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“…For electron microscopy scanning, complete nodules were randomly selected and soaked in liquid nitrogen, the specimens were then coated with Epon812 and thin sections were cut with a cold knife on a microtome, and the nodules were viewed and photographed using a (Hitachi TM-1000, Japan) scanning electron microscope at 15 kV accelerating voltages (Huang et al 2011).…”

Section: Root Nodule Ultra-structures By Scanning Electron Microscopymentioning

confidence: 99%

Study of phenanthrene utilizing bacterial consortia associated with cowpea (Vigna unguiculata) root nodules

Sun

Crowley

Wei

2015

World J Microbiol Biotechnol

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Many legumes have been selected as model plants to degrade organic contaminants with their special associated rhizosphere microbes in soil. However, the function of root nodules during microbe-assisted phytoremediation is not clear. A pot study was conducted to examine phenanthrene (PHE) utilizing bacteria associated with root nodules and the effects of cowpea root nodules on phytoremediation in two different types of soils (freshly contaminated soil and aged contaminated soil). Cowpea nodules in freshly-contaminated soil showed less damage in comparison to the aged-contaminated soil, both morphologically and ultra-structurally by scanning electron microscopy. The study of polycyclic aromatic hydrocarbon (PAH) attenuation conducted by high performance liquid chromatography revealed that more PAH was eliminated from liquid culture around nodulated roots than nodule-free roots. PAH sublimation and denaturation gradient gel electrophoresis were applied to analyze the capability and diversity of PAH degrading bacteria from the following four parts of rhizo-microzone: bulk soil, root surface, nodule surface and nodule inside. The results indicated that the surface and inside of cowpea root nodules were colonized with bacterial consortia that utilized PHE. Our results demonstrated that root nodules not only fixed nitrogen, but also enriched PAH-utilizing microorganisms both inside and outside of the nodules. Legume nodules may have biotechnological values for PAH degradation.

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Micropropagation of the bromeliad Guzmania ‘Hilda’ via organogenesis and the effect of α-naphthaleneacetic acid on plantlet elongation

Cited by 7 publications

References 29 publications

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Study of phenanthrene utilizing bacterial consortia associated with cowpea (Vigna unguiculata) root nodules

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