Micropropagation of the pine hybrid Pinus brutia (Ten) × Pinus halepensis (Mill) by culturing fascicle shoots

Abstract

Summary — Fascicle shoots proved to be an ideal plant material for micropropagation of the pine hybrid P brutia (Ten) x P halepensis (Mill). This could be possibly attributed to their morphological and physiological state. Induced fascicle shoots of 4-yr-old seedlings were used as explants. Their induction was achieved by spraying once with the new herbicide Arsenal (1 000 mg I -1 ). First, explants were elongated in vitro on LP medium and then transferred to the multiplication stage. Multiplication was accomplished by decapitating, quick-dipping in 0.22 mM BA and inoculating the explants on BIMI medium. On the induced first-generation shoots the same procedure was applied, in order to obtain second-generation shoots and then these were proceeded to the rooting and acclimatization stages. In particular, when microcuttings were pretreated with 2.46 μM IBA + 2.7 μM NAA + 0.65% agar w/v + 1.5% sucrose w/v for 7 d and then transferred to greenhouse conditions, a good root system was developed within an 8-12-week period. A large variation in rootability was noted between clones. The above method may be proved to be efficient for clones that exhibit high rooting ability.

Micropropagation Via Axillary Bud Proliferation from Seedlings and Juvenile Shoots ofPinus patulaSchiedeetDeppe

“…The seedlings were and Tainter, 1991), juvenile/ fascicle shoots (e.g. watered twice daily and fertilised (N 21,2 %, P 7,1 %, Scaltsoyiannes et al, 1994) and mature shoots (e.g.…”

Adventitious rooting of conifers: influence of physical and chemical factors

“…The majority of studies relating to ARF applied light intensities that varied from 60 to 80 lmol m -2 s -1 (Gómez and Segura 1994;Scaltsoyiannes et al 1994;Nandwani et al 2001;Zhang et al 2006;Ishii et al 2007). The influence of light (quality) on ARF in shoots of other species is listed in Table 2.…”

“…Different types of light sources such as Growth-lux (GL) (Gómez and Segura 1994), high-pressure lamps (Scaltsoyiannes et al 1994) and their combinations such as fluorescent light supplemented with incandescent light (Flygh et al 1998) are also applied. Different light quality influenced the rooting frequencies according to the PPFD used.…”

Evaluation of Clonal Variability in Shoot Coppicing Ability and in vitro Responses of Dalbergia sissoo Roxb

“…Clones 12 and 40 exhibited higher rooting percentages as well as number. Role of Kalia et al·Silvae Genetica (2004) 53-5/6, 212-220 genotypic variations in rooting has been encountered in studies of different research groups (HORGAN and HOLLAND, 1989;BERGMANN and STOMP, 1994;SCALTSOYIANNES et al, 1994). They recorded variation in percentage of shoots rooted and number of roots per shoot.…”