Micropropagation of Two Selected Male Kiwifruit and Analysis of Genetic Variation with AFLP Markers

Prado, María Jesús; Herrera, M.T.; Vázquez, Ricardo; Romo, Silvia; González, María Victoria

doi:10.21273/hortsci.40.3.740

Cited by 20 publications

(9 citation statements)

References 22 publications

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“…6), taking into account that the initial genetic variability was narrow since a single plant of each genotype was used. This genetic variation was higher than that reported by Palombi and Damiano (2002) using micropropagated kiwifruit plants, or than that observed by our own group in micropropagated plants of the same genotypes used in the present study (Prado et al 2005). This may reflect the higher genetic variation expected when indirect organogenesis is achieved (Bouman and De Klerk 1997;Yamagishi et al 1996) perhaps due to a higher rate of cell proliferation, implying more cell divisions, as well as longer culture periods.…”

Section: Somaclonal Variation Analysiscontrasting

confidence: 66%

Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation

Prado

González²,

Romo³

et al. 2006

Plant Cell Tiss Organ Cult

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The present work reports on a study of plant regeneration carried out with callus from the leaf blades and petioles of field-grown male adult kiwifruit plants (Actinidia deliciosa (Chev.) Liang and Ferguson). The cultivars used were 'Tomuri' and clone A, a selected male plant grown in north western Spain. The best shoot induction conditions were obtained in 'Tomuri' leaf blades cultured in K(h) medium in the presence of 23 lM Zeatin and 0.1 lM NAA. Under these conditions, more than 80% of organogenic callus induction was observed, with an average of 14 new shoots in the second subculture. The initial length of the shoots affected shoot elongation, which was accomplished by culturing isolated shoots in K(h) medium with half-strength salts, supplemented with 0.4 lM Zeatin and 0.1 lM NAA. A possible detrimental long-term effect of cytokinins on shoot elongation can account for the results, since elongation was not observed until 1 month of culture in elongation medium.For rooting, shoots (1 cm in length) were basally immersed in a 5 mM IBA solution for 15 s, and transferred to half-strength K(h) basal medium. Regenerated plants were acclimated in a sterile peat:perlite substrate for 10 days, and then transferred to soil. AFLP analysis was accomplished with 15 primer combinations from which 13 showed reproducible and well-resolved bands, producing a total of 1321 fragments from which 1281 were polymorphic (97%). A dendrogram was constructed using both monomorphic and polymorphic bands, showing genetic variation among field-grown plants and tissue culture-derived regenerants.

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Section: Somaclonal Variation Analysiscontrasting

confidence: 66%

Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation

Prado

González²,

Romo³

et al. 2006

Plant Cell Tiss Organ Cult

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“…The AFLP technique has been widely used to identify cultivars and germplasms in many fruit crops such as apple (Kenis and Keulemans, 2005), citrus (Pang et al, 2007), and kiwifruit (Prado et al, 2005). AFLP markers have some advantages over other molecular markers such as a high level of polymorphism, high reproducibility, a wide distribution of markers in the genome, and a lack of prerequisite sequences (Mueller and Wolfenbarger, 1999).…”

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confidence: 99%

Amplified Fragment Length Polymorphism Analysis of Genetic Diversity and Relationships of Wild and Cultivated Peach (Prunus persica L.)

Meng

Peng

Guan

2015

horts

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To date, a narrow genetic base is a serious obstacle in peach (Prunus persica L.) production. Wild peach resources are useful germplasms for breeding new cultivars. In this study, amplified fragment length polymorphisms (AFLPs) were used to analyze the genetic diversity and relationships of wild and cultivated peach germplasms. These results showed that AFLP is an efficient technique for identifying the genetic relationships of wild and cultivated peach. Thirteen AFLP primer combinations generated a total of 377 scorable and clear fragments, all of which (100%) were polymorphic. Moreover, the polymorphism information content (PIC) values ranged from 0.91 to 0.96 with a mean of 0.95. The results of the principal component analysis (PCoA) largely corresponded to those obtained using cluster analysis. The three principal axes accounted for 2.6%, 5.79%, and 25.26% of the total variation, respectively. In conclusion, wild peach germplasms should receive special attention to ensure their conservation.

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“…Our results show that P. maculatum is prone to variability caused by conditions of in vitro cultivation, even if the callus stage is omitted. Somaclonal variants were already reported in shoot cultures of different species [ 49 , 50 , 51 ]. This lack of genetic stability can be recognized as disadvantageous for conservation purposes.…”

Section: Discussionmentioning

confidence: 99%

Sourcing and Propagation of Pontechium maculatum for Horticulture and Species Restoration

Nowak

Sitek

Augustynowicz

2020

Biology

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Pontechium maculatum, a species of ornamental, apicultural, health and medicinal value, is threatened in some Central European countries including Poland. Its propagation using seeds or in vitro techniques is needed for multiple applications including conservation. Generative propagation efficacy of P. maculatum plants representing different genetic resources (received from botanical gardens in Germany and in Poland) propagated from seeds or in tissue culture was assessed. Moreover, an efficient technique of propagation of P. maculatum using in vitro shoot culture from seedlings was elaborated for the first time. The highest propagation efficacy was noted for German plants of seed origin. The ability of seeds to germinate was similar for all plants; however, seeds were in a state of dormancy, which was broken by GA3. After two years of storage, the seeds still retained the ability to germinate though seeds from propagated in vitro plants germinated more poorly than those from seed-originated plants. The ploidy assessment showed that some in vitro-origin plants had altered DNA content. The results indicate that efficacy of generative propagation of P. maculatum is resource dependent. Furthermore, results suggest that cultivation in vitro influenced some generative features of examined species, which makes this way of P. maculatum propagation a valuable source of genetic variation and a potential breeding tool.

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Micropropagation of Two Selected Male Kiwifruit and Analysis of Genetic Variation with AFLP Markers

Cited by 20 publications

References 22 publications

Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation

Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation

Amplified Fragment Length Polymorphism Analysis of Genetic Diversity and Relationships of Wild and Cultivated Peach (Prunus persica L.)

Sourcing and Propagation of Pontechium maculatum for Horticulture and Species Restoration

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