Micropropagation of Vitis vinifera L. cv. ‘Malagouzia’ and ‘Xinomavro’

Abstract

Abstract:The effects of six basal media on in vitro shoot proliferation of the greek grapevines Vitis vinifera L. cv. 'Malagouzia' and 'Xinomavro' were investigated. Galzy and Zlenco proved to be the most effective for 'Malagouzia' and 'Xinomavro', respectively. If only BA was present in the medium, shoot development was poor and the plantlets were chlorotic. When the medium was supplemented with BA and NAA, growth was enhanced. The best ratio (in μM) of growth regulators was 0.5/0.3 for 'Malagouzia', and 0.1/0.03 for 'Xinomavro', which resulted in the highest number of microshoots per explant and greatest proliferation rate. The development of 'Malagouzia' and 'Xinomavro' explants at 21±2 and 26±2°C was also investigated, revealing the higher temperature to be more effective. Regarding rooting, 0.5 μM IBA improved root formation at 26°C for 'Malagouzia' and 0.5 μM IBA at 21°C for 'Xinomavro'. Moreover, 0.5 μM IBA resulted in a higher rooting percentage (>95%) and proved to be more beneficial for the overall morphological appearance of the plantlets of 'Malagouzia'. After acclimatization, survival of microshoots cultivated in media with IBA was higher than those in NAA.

In vitro plantlet production of the endangered Pinguicula vulgaris

“…The author used leaf explants as starting material and found that the highest plantlet number and growth rate were obtained using 1 / 5 Linsmaier-Skoog medium supplemented with a combination of BA (0.02 mg/L) and 2-naphthaleneacetic acid (0.01-0.10 mg/L). Although several authors have reported a synergistic effect between cytokinins and auxins during shoot proliferation [15,16], in this work culture media combining BA or Zea (0.1 mg/L) with IBA (0.01 mg/L) were not effective in enhancing proliferation (P≥0.05) (Figure 2), which is in good agreement with the results obtained for P. lusitanica [10].…”

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

“…Hence, developing an efficient in vitro technique for the propagation of pomegranate is of great importance. During the past decade, tissue culture techniques have been widely used for the propagation of some important tropical and subtropical fruit trees (Agarwal et al 2004;Tsukamoto et al 2007;PerezTornero et al 2010;Skiada et al 2010).…”

Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

“…It is well known that shoot proliferation and rooting efficiency in Vitis spp. are affected by the type and concentration of cytokinin and auxin (Gray and Benton, 1991;Skiada et al, 2010). The efficiency of in vitro propagation can differ based on the genetic characteristics of the species, making it important to test their effects and to select the optimal PGR conditions.…”