Micropropagation of Weigela florida ‘Tango’ through In Vitro Shoot Culture

Wang, Xiaoming; Li, Yongxin; Zeng, Huijie; Neng, Cai; Qiao, Zhongquan; Wang, Xiangying; Chen, Jianjun

doi:10.21273/hortsci11413-16

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…kamtschatica. In a protocol of other micropropagated shrubs from Caprifoliaceae, namely Kolkwitzia and Weigela, the employment of BAP with a low concentration of auxin was preferred for the stimulation of the development of new buds [31,32].…”

Section: Discussionmentioning

confidence: 99%

Linnaea borealis L. var. borealis—In Vitro Cultures and Phytochemical Screening as a Dual Strategy for Its Ex Situ Conservation and a Source of Bioactive Compounds of the Rare Species

et al. 2021

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Linnaea borealis L. (Twinflower)—a dwarf shrub in the Linnaeeae tribe of Caprifoliaceae family—is distributed across the Northern Hemisphere. By means of this study, a reliable protocol for efficient micropropagation of uniform L. borealis L. var. borealis plantlets has been provided for the first time; callus culture was also established. Different initial explants, types of cultures, media systems, and plant growth regulators in Murashige and Skoog (MS) media were tested. Agitated shoot cultures in the liquid media turned out to be the best system for the production of sustainable plant biomass. After stabilization of the callus lines, the highest growth index (c.a. 526%) was gained for callus maintained on MS enriched with picloram. TLC and UHPLC-HESI-HRMS analysis confirmed the presence of phenolic acids and flavonoids, and for the first time, the presence of iridoids and triterpenoid saponins in this species. Multiplication of L. borealis shoot culture provides renewable raw material, allowing for the assessment of the phytochemical profile, and, in the future, for the quantitative analyses and the studies of the biological activity of extracts, fractions, or isolated compounds. This is the first report on in vitro cultures of traditionally used L. borealis rare taxon and its biosynthetic potential.

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Section: Discussionmentioning

confidence: 99%

Linnaea borealis L. var. borealis—In Vitro Cultures and Phytochemical Screening as a Dual Strategy for Its Ex Situ Conservation and a Source of Bioactive Compounds of the Rare Species

et al. 2021

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Adaptability and prospects for the use of introduced representatives of the genus Weigela in different climatic conditions

Hovakimyan,

Muradyan,

Gatrchyan

et al. 2024

Regul. Mech. Biosyst.

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The use of highly decorative and beautifully flowering shrubs in urban green spaces, especially in sharply continental climatic conditions, is extremely important. In this regard, representatives of the genus Weigela are highly valued in the in different climatic conditions. The main indicators of eco-physiological adaptability of plants, such as intensity of transpiration and photosynthetic productivity, water deficiency and the total water content, quantity of the plastids, have been studied for the representatives of highly ornamental shrubs of the genus Weigela Thumb. The object of the research was the following representatives of the genus Weigela: W. praecox, W. coraeensis, W. floribunda, W. florida and W. florida ʺVariegataʺ, which were introduced into different climatic conditions in order to climate train and replenish the dendro-collections. Physiological research was conducted, during the vigorous vegetative period (May–June months). All the measurements were carried out within the 11:00–13:00 time period, in 9 repetitions (7–10 shoots were chosen for each sample). The nature of ecological adaptability of the studied species, along with the perspective of their application in the landscaping practices in different climatic zones was revealed. At the same time it was revealed that the studied species can be used not only as solitary plants (especially on the background of green lawns) but also in groups in long blooming gardens (with flowers with different colours and blooming phases) and in decorating rock gardens.

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