Micropropagation of Zeyheria montana Mart. (Bignoniaceae), an endangered endemic medicinal species from the Brazilian cerrado biome

Micropropagation of Incarvillea delavayi Bureau et Franchet (Bignoniaceae)

“…Among the species of the Bignoniaceae family, NAA had similar significant effect on rooting in Tecomella undulate (Kumari and Singh, 2012), and in combination with indole-3-acetic acid (IAA) in Oroxylum indicum (Dalal and Rai, 2004). In the latter study, indole-3butyric acid (IBA), which is commonly used for rooting in trees from the Bignoniaceae family (Gonzalez-Rodriguez et al, 2010;Shukla et al, 2009;Cardoso and da Silva, 2013) Mean values in a column, followed by different letters, were significantly different according to the Tukey's HSD test (P < 0.05)…”

“…In the family Bignoniaceae, similar concentrations of BAP were successfully used for axillary bud proliferation and shoot multiplication of medicinally and economically important timber tree Tecomella undulata (Chhajer and Kalia, 2016). On the contrary, in Zeyheria montana from the same botanical family, very low concentration, i.e., 0.1 mg l -1 BAP applied together with 0.5 mg l -1 gibberellic acid (GA 3 ) proved to be optimal for production of new shoots from nodal segments (Cardoso and da Silva, 2013). Likewise, in medicinal tree species Stereospermum personatum, 0.1 mg l -1 BAP produced the maximum of shoots from explant (Shukla et al, 2009).…”

Qualitative and Quantitative Evaluation Protocol of Baccharis Seed Germination

“…The partial germination percentage and the final germination percentage were calculated (G = G1/N1 + G2/N2 + ... + Gn/Nn, where Gn = number of seeds with root protrusion; Nn = number of days of sowing) (Cardoso et al, 2013;Maguire, 1962;Rossi et al, 2018), considering the beginning of germination the emergence of a radicle. Regression curves were performed with the germination indexes, and the data were grouped at 7-day intervals, so the first partial germination was calculated at 7 days (N1 = 7 d), the second at 14 days (N2 = 14 d), the third at 21 days (N3 = 21 d), the fourth at 28 days (N4 = 28 d), and the fifth at 35 days (N5 = 35 d).…”

“…Germination Velocity (VE) was determined by the Equation 2 (Asgarpour et al, 2015;Bai & Romot, 1995;Bazin et al, 2011;Cardoso et al, 2013;James, 1962;Ranal & Santana, 2006;Souza et al, 2018):…”

“…The mean germination time is a periodicity, defined as the multiplicative inverse of the mean germination time, (Bai & Romot, 1995;Bazin et al, 2011;Cardoso et al, 2013;Melo et al, 2018;Ranal et al, 2006), determined by Equation 4:…”