Micropropagation Studies in Papaya (Carica papaya L.) cv. ‘Surya’

Ryavalad, Shivayogi; Malabasari, T. A.; Shantappa, T; Uppar, D.S.; Biradar, B. D.; Mantur, S. M.

doi:10.20546/ijcmas.2019.804.275

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…It is clear that the proliferation medium (PPRM) with a molar ratio (MR) of BA/NAA: 4.13 promoted a good proliferation rate, significantly higher (24 shoots/explant) than the other media assayed. This is consistent with the findings from other research: Reuveni et al [38] working with MS 0.5 mg L −1 BA + 1 mg L −1 NAA + 160 mg L −1 ADE (MR:12); Rajeevan and Pandey [39] with MS + 2 µM BA (MR: 15.8); McCubbin and van Staden [40] (2003) using DS salts and vitamins + 1 mg L −1 NAA + 1 mg L −1 BA + 3 g L −1 activated charcoal (MR: 3.5); and Shivayogi et al [41] using MS + 2.0 mg L −1 BA + 1.0 mg L −1 NAA (MR: 19.9). The average results for PPRM of >24 shoots/explant were quite close to the 22 shoots/explant obtained by Anandan et al [42] and the 25 shoots/explant obtained by Mumo et al [43], using MS supplemented with 1 mg L −1 BA plus 0.01 mg L −1 NAA (MR: 82.6) or MS supplemented with 0.5 mg L −1 BA plus 0.1 mg L −1 NAA (MR: 4.13).…”

Section: Culture Initiation and In Vitro Behavior During The First 2 ...mentioning

confidence: 99%

“…Caple and Cheah [49], working with MS + 1 mg L −1 IBA + 4% sucrose, obtained a high rate of multiplication (65-95%). Shivayogi et al [41] used MS + 3 mg L −1 NAA and achieved 80% rooting. However, even in the PPRM medium supplemented exclusively with NAA, we obtained an average rooting of 63.1% with peaks as high as 84%.…”

Section: Rootingmentioning

confidence: 99%

“…McCubbin and van Staden [40] and Shivayogi et al [41] indicated success with acclimatization but without data regarding the percentage.…”

Section: Plantlet Acclimatizationmentioning

confidence: 99%

“…The good quality of the roots obtained by Perez et al [48] produced a high survival rate (96.5%). McCubbin and van Staden [40] and Shivayogi et al [41] indicated success with acclimatization but without data regarding the percentage.…”

Section: Plantlet Acclimatizationmentioning

confidence: 99%

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In Vitro Long-Term Cultures of Papaya (Carica papaya L. cv. Solo)

2023

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In this article, we present proliferation data from 10 years of the continuous in vitro incubation of cv. Solo papaya shoots and propose a reliable method for the long-term micropropagation of papaya, using microshoots developed from the axillary buds of papaya shoots as primary explants. Three different media were assayed. The proliferation medium (PPRM) allowed us to maintain papaya shoots under continuous proliferation for 20 years, maintaining consistent behavior. Most of the shoots developed in the PPRM rooted during the incubation and then acclimated easily, maintaining the ploidy and morphological characteristics of the parental plants, and flowering and setting fruits normally. The PPRM medium consisted of MS medium supplemented with naphthalene acetic acid (NAA) (0.1 mg L−1), benzyladenine (BA) (0.5 mg L−1), gibberellic acid (GA3) (0.5 mg L−1), and adenine hemisulphate (40 mg L−1). The average multiplication rate was higher than 20 shoots per explant during the long-term assay. The elongation medium (PELM) was designed to recover shoots with poor growth and allowed the development of high-quality shoots ready for rooting. It consisted of an MS basal medium supplemented with NAA (0.1 mg L−1), kinetin (KIN) (0.5 mg L−1), and GA3 (1 mg L−1). The rooting medium (PROM) was designed to induce high-quality roots from nonrooted shoots and consisted of a half-strength MS medium and indole-3-butiryc acid (IBA) (1 mg L−1). On PROM, agar can be exchanged for expanded vermiculite. Acclimation took place inside an acclimatization tunnel under progressive hydric stress. After 4 weeks, the plant recovery rate was 90% for plants maintained under continuous proliferation for ten years. The main objective of this work was to provide a micropropagation method which would maintain healthy elite genotypes of papaya for long periods of time and produce a high number of good quality plants.

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