Microribonucleic Acid-15a-5p Alters Adriamycin Resistance in Breast Cancer Cells by Targeting Cell Division Cycle-Associated Protein 4

Zhang, Jiangtao; Chen, Jun; Ruan, Hui-Chao; Li, Feng-Xi; Pang, Sen; Xu, Yuju; Huang, Daolai; Wu, Xianghua

doi:10.2147/cmar.s333830

Cited by 5 publications

(7 citation statements)

References 34 publications

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“…cDCA4 has the potential to be both diagnostic and prognostic. [19][20][21] CDCA4, also known as SEI-3/HEPP. SEI-1, SEI-2, and CDCA4 have structural and functional similarities.…”

Section: Discussionmentioning

confidence: 99%

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CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathway

et al. 2023

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Background Lung adenocarcinomas (LUAD) remain the leading cause of death in many countries. In this study, we investigated the role of division cycle‐associated 4 (CDCA4) in the carcinogenesis of LUADs. Methods Real‐time fluorescent quantitative polymerase chain reaction and western blot were performed to detect the messenger RNA and protein levels of CDCA4 in cells. Cell counting kit 8, real‐time cell analysis, clone formation, EdU assays, and cell‐cycle assays were used to preliminarily investigate the proliferation and cell‐cycle–related functions of CDCA4 in lung adenocarcinoma. Immunoprecipitation assays were used to identify possible targets of CDCA4. A xenograft model was used to examine how CDCA4 knockdown affects LUAD cells growth in vivo. Results We found that the expression of CDCA4 was upregulated in LUAD cell lines. When CDCA4 was knocked out, the ability of LUAD cells to proliferate was dramatically reduced, and the cell cycle was stalled in the S phase. Meanwhile, boosting the CDCA4 expression had the opposite effect. The critical protein levels of phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling pathway were subsequently examined. The findings demonstrated that elevated CDCA4 lowered the phosphate and tensin homolog expression and increased the p‐PI3K and p‐AKT levels. Moreover, we demonstrated that CDCA4 favorably regulated IGF2BP1, a downstream target. The downregulation of the IGF2BP1 expression could reverse the proliferation promotion effect induced by the CDCA4 overexpression. Conclusions CDCA4 can operate as an oncogenic factor to control the growth of lung adenocarcinoma via the PI3K/AKT pathway.

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“…cDCA4 has the potential to be both diagnostic and prognostic. [19][20][21] CDCA4, also known as SEI-3/HEPP. SEI-1, SEI-2, and CDCA4 have structural and functional similarities.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, CDCA4 can function as a target gene in the control of the cell cycle and tumor cell resistance, whereas the CDCA4 expression is directly correlated to the level of immune infiltration. cDCA4 has the potential to be both diagnostic and prognostic 19–21 …”

Section: Discussionmentioning

confidence: 99%

CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathway

et al. 2023

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“…Kang et al observed downregulation in gastric carcinoma, 22 Yun et al in pancreatic ductal adenocarcinoma, 23 and Zhang et al in breast cancer cells. 24 A meta-analysis conducted on miRNA-15a-5p concluded that it is downregulated in most carcinomas due to the deletion of 13q14 and is associated with overall survival rates. However, another set of results showed that upregulated expression of miRNA-15a-5p also contributes to tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

“…The literature search supports similar findings in various other carcinomas. Kang et al observed downregulation in gastric carcinoma, 22 Yun et al in pancreatic ductal adenocarcinoma, 23 and Zhang et al in breast cancer cells 24 . A meta‐analysis conducted on miRNA‐15a‐5p concluded that it is downregulated in most carcinomas due to the deletion of 13q14 and is associated with overall survival rates.…”

Section: Discussionmentioning

confidence: 99%

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Evaluating the expression of microRNA‐15a‐5p and YAP1 gene in oral squamous cell carcinoma in comparison with normal tissue: A cross‐sectional study

Saravana Murthy,

Kannan,

Ganesan

et al. 2023

J Oral Pathology Medicine

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BackgroundMicroRNAs (miRNAs) play crucial regulatory roles in cancer progression, including oral cancer (OC). However, the biological mechanisms of miRNA‐15a‐5p in OC remain unclear. This study aimed to evaluate the expression of miRNA‐15a‐5p and the YAP1 gene in OC.MethodsA total of 22 clinically and histologically confirmed oral squamous cell carcinoma (OSCC) patients were recruited, and their tissues were stored in a stabilizing solution. Later, RT‐PCR was performed to evaluate miRNA‐15a‐5p and the targeting gene YAP1. The results of OSCC samples were compared with unpaired normal tissues.ResultsThe normality tests, Kolmogorov–Smirnov and Shapiro–Wilk, revealed a normal distribution. Inferential statistics were performed using an independent sample t‐test/unpaired t‐test among the study intervals to compare the expression of miR‐15a and YAP1. SPSS (IBM SPSS Statistics for Windows, Version 26.0, Armonk, NY: IBM Corp. Released 2019) was used to analyse the data. The significance level was set at 5% (α = 0.05), and a p‐value <0.05 was considered statistically significant. The expression of miRNA‐15a‐5p was lower in OSCC than in normal tissue, whereas the opposite was observed for YAP1 levels.ConclusionIn conclusion, this study demonstrated that miRNA‐15a‐5p was downregulated and YAP1 was overexpressed, which had a statistically significant difference between the normal and OSCC groups. Therefore, miRNA‐15a‐5p may serve as a novel biomarker to better understand the pathology and as a potential target in OSCC therapy.

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Role of cell division cycle-associated proteins in regulating cell cycle and promoting tumor progression

Wang,

Ren,

Liu

et al. 2024

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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Microribonucleic Acid-15a-5p Alters Adriamycin Resistance in Breast Cancer Cells by Targeting Cell Division Cycle-Associated Protein 4

Cited by 5 publications

References 34 publications

CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathway

CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathway

Evaluating the expression of microRNA‐15a‐5p and YAP1 gene in oral squamous cell carcinoma in comparison with normal tissue: A cross‐sectional study

Role of cell division cycle-associated proteins in regulating cell cycle and promoting tumor progression

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