MicroRNA-1 Targets Ribosomal Protein Genes to Regulate the Growth, Development,&amp;nbsp;And Reproduction of Schistosoma Japonicum

Sun, Chengsong; Luo, Fang; You, Yanmin; Gu, Meijia; Yang, Wu; Yi, Cun; Zhang, Wei; Feng, Zheng; Wang, Jipeng; Wei, Hu

doi:10.2139/ssrn.4282744

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The qPCR and in situ hybridization experiments in Schistosoma japonicum demonstrated that ribosomal protein genes were enriched in the sexual organs of adult females and could affect growth, reproduction, and oviposition. 37 Moreover, ribosomal protein L11 is involved in egg formation in Phytoseiulus persimilis, 38 while ribosomal protein 27 silencing in tick eggs led to abnormalities in shape and hatching. 39 These data suggest that ribosomal proteins are important molecules that play multiple roles in the reproduction of insects.…”

Section: Discussionmentioning

confidence: 99%

Iron regulatory protein from the hard tick Haemaphysalis longicornis: characterization, function and assessment as a protective antigen

Wang,

Zhang,

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDTicks are blood‐feeding ectoparasites with different host specificities and are capable of pathogen transmission. Iron regulatory proteins (IRPs) play crucial roles in iron homeostasis in vertebrates. However, their functions in ticks remain poorly understood. The aim of the present study was to investigate the characteristics, functions, molecular mechanisms, and the vaccine efficacy of IRP in the hard tick H. longicornis.RESULTSThe full‐length cDNA of IRP from H. longicornis (HlIRP) was 2973 bp, including a 2772 bp open reading frame. It is expressed throughout three developmental stages (larvae, nymphs, and adult females) and in various tissues (salivary glands, ovaries, midgut, and Malpighian tubules). Recombinant H. longicornis IRP (rHlIRP) was obtained via a prokaryotic expression system and exhibited aconitase, iron chelation, radical‐scavenging, and hemolytic activities in vitro. RNAi‐mediated IRP knockdown reduced tick engorgement weight, ovary weight, egg mass weight, egg hatching rate, and ovary vitellin content, as well as prolonging the egg incubation period. Proteomics revealed that IRP may affect tick reproduction and development through proteasome pathway‐associated, ribosomal, reproduction‐related, and iron metabolism‐related proteins. A trial on rabbits against adult H. longicornis infestation demonstrated that rHlIRP protein vaccine could significantly decrease engorged weight (by 10%), egg mass weight (by 16%) and eggs hatching rate (by 22%) of ticks. The overall immunization efficacy using rHlIRP protein against adult females was 41%.CONCLUSIONIRP could limit reproduction and development in H. longicornis, and HlIRP was confirmed as a candidate vaccine antigen to impair tick iron metabolism and protect the host against tick infestation.

show abstract

Section: Discussionmentioning

confidence: 99%