MicroRNA-101-2-5p targets the <i>ApoB</i> gene in the liver of chicken (<i>Gallus Gallus</i>)

Ma, Zheng; Li, Hong; Zheng, Hua; Jiang, Keren; Jia, Lijuan; Yan, Fengbin; Tian, Yadong; Kang, Xin; Wang, Yanbin; Liu, Xiaojun

doi:10.1139/gen-2017-0020

Cited by 17 publications

(14 citation statements)

References 36 publications

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“…miR-33 can regulate the biological metabolism process of the liver by affecting cholesterol efflux and high-density lipoprotein metabolism [12]. Gga-miR-22-3p and gga-miR-101-2-5p are involved in the hepatic lipid metabolism by targeting the key factors Elovl6 and Apolipoprotein B (ApoB) of the lipid metabolism pathway [13,14]. The differential expression of miRNAs has been reported in the liver tissues of pre-laying and peak-laying hens [15], and obese and non-obese human individuals [16,17].…”

Section: Introductionmentioning

confidence: 99%

Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver

Zhang

Wang

et al. 2020

IJMS

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Few studies have been conducted regarding the biological function and regulation role of gga-miR-221-5p in the liver. We compared the conservation of miR-221-5p among species and investigated the expression pattern of gga-miR-221-5p, validating the direct target genes of gga-miR-221-5p by dual luciferase reporter assay, the biological function of gga-miR-221-5p in the liver was studied by gga-miR-221-5p overexpression and inhibition. Furthermore, we explored the regulation of gga-miR-221-5p and its target genes by treatment with estrogen and estrogen antagonists in vivo and in vitro. The results showed that miR-221-5p was highly conserved among species, expressed in all tested tissues and significantly downregulated in peak-laying hen liver compared to pre-laying hen liver. Gga-miR-221-5p could directly target the expression of elongase of very long chain fatty acids 6 (ELOVL6) and squalene epoxidase (SQLE) genes to affect triglyceride and total cholesterol content in the liver. 17β-estradiol could significantly inhibit the expression of gga-miR-221-5p but promote the expression of ELOVL6 and SQLE genes. In conclusion, the highly conservative gga-miR-221-5p could directly target ELOVL6 and SQLE mRNAs to affect the level of intracellular triglyceride and total cholesterol. Meanwhile, 17β-estradiol could repress the expression of gga-miR-221-5p but increase the expression of ELOVL6 and SQLE, therefore promoting the synthesis of intracellular triglyceride and cholesterol levels in the liver of egg-laying chicken.

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Section: Introductionmentioning

confidence: 99%

Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver

Zhang

Wang

et al. 2020

IJMS

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“…We identified miR-22-3p as the most abundant SDE miRNA in chicken liver, playing a key role in the development of fatty liver in mice by steering the target genes and significantly increasing lipid accumulation in human hepatoma (HepG2) cells [61]. Our previous works have shown that miR-22-3p and miR-101-2-5p are involved in chicken hepatic lipid metabolic processes through targeting related genes [62,63]. As the potential target of miR-22-3p, lncRNA ENSGALT00000080371 might also be related to chicken hepatic lipid metabolism.…”

Section: Discussionmentioning

confidence: 97%

Transcriptome Profile Analysis Reveals an Estrogen Induced LncRNA Associated with Lipid Metabolism and Carcass Traits in Chickens (Gallus Gallus)

Yang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Accumulating evidences have demonstrated that long noncoding RNAs (lncRNA) play important roles in hepatic lipid metabolism in mammals. However, no systematic screening of the potential lncRNAs in the livers of laying hens has been performed, and few studies have been reported concerning the effects of the lncRNAs on lipid metabolism in the livers of chickens during egg-laying period. The purpose of this study was to compare the difference in lncRNA expression in the livers of pre-laying and peak-laying hens at the age of 20 and 30 weeks old by transcriptome sequencing and to investigate the interaction networks among lncRNAs, mRNAs and miRNAs. Moreover, the regulatory mechanism and biological function of lncLTR, a significantly differentially expressed lncRNA in the liver between pre- and peak-laying hens, was explored in vitro and in vivo. Methods: Bioinformatics analyses were conducted to identify the differentially expressed (DE) lncRNAs between the two groups of hens. The target genes of the DE lncRNA were predicated for further functional enrichment. An integrated analysis was performed among the DE lncRNA datasets, DE mRNAs and DE miRNA datasets obtained from the same samples to predict the interaction relationship. In addition, in vivo and in vitro trials were carried out to determine the expression regulation of lncLTR, and polymorphism association analysis was conducted to detect the biological role of ncLTR. Results: A total of 124 DE lncRNAs with a P-value ≤ 0.05 were identified. Among them, 44 lncRNAs including 30 known and 14 novel lncRNAs were significant differentially expressed (SDE) with FDR ≤ 0.05. Thirty-two lncRNAs were upregulated and 12 were downregulated in peak-laying group compared with pre-laying group. The functional enrichment results revealed that target genes of some lncRNAs are involved in the lipid metabolism process. Integrated analysis suggested that some of the genes involved in lipid metabolism might be regulated by both the lncRNA and the miRNA. In addition, an upregulated lncRNA, designated lncLTR, was demonstrated to be induced by estrogen via ERβ signaling. The c242. G>A SNP in lncLTR was significantly associated with chicken carcass weight, evisceration weight, semi-evisceration weight, head weight, double-wing weight, claw weight traits, and blood biochemical index, especially for the blood triglyceride content. Conclusion: A series of lncRNAs associated with lipid metabolism in the livers of chickens were identified by transcriptome sequencing and functional analysis, providing a valuable data resource for further studies on chicken hepatic metabolism activities. LncLTR was regulated by estrogen via ERβ signaling and associated with chicken carcass trait and blood triglyceride content.

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“…Hsa-mir-101-2 is encoded by a gene located in 9p21; studies by Ma et al reveal that miR-101-2-5p was involved in lipid metabolism through binding with the 3′-untranslated region of the ApoB gene in the liver of egg-laying chickens. 67 Furthermore, work by Bae et al suggests that genetic variations of miR-101-2 had a significant association with the clearance of HBV infection. 68 In cancer research, genetic variations of miR-101-2 were associated with significantly increased risk of genetic susceptibility in breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Identification of potential prognostic microRNA biomarkers for predicting survival in patients with hepatocellular carcinoma

Liao¹,

Zhu²,

Huang³

et al. 2018

CMAR

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BackgroundThe aim of the present study was to identify potential prognostic microRNA (miRNA) biomarkers for hepatocellular carcinoma (HCC) prognosis prediction based on a dataset from The Cancer Genome Atlas (TCGA).Materials and methodsA miRNA sequencing dataset and corresponding clinical parameters of HCC were obtained from TCGA. Genome-wide univariate Cox regression analysis was used to screen prognostic differentially expressed miRNAs (DEMs), and multivariable Cox regression analysis was used for prognostic signature construction. Comprehensive survival analysis was performed to evaluate the prognostic value of the prognostic signature.ResultsFive miRNAs were regarded as prognostic DEMs and used for prognostic signature construction. The five-DEM prognostic signature performed well in prognosis prediction (adjusted P < 0.0001, adjusted hazard ratio = 2.249, 95% confidence interval =1.491–3.394), and time-dependent receiver–operating characteristic (ROC) analysis showed an area under the curve (AUC) of 0.765, 0.745, 0.725, and 0.687 for 1-, 2-, 3-, and 5-year HCC overall survival (OS) prediction, respectively. Comprehensive survival analysis of the prognostic signature suggests that the risk score model could serve as an independent factor of HCC and perform better in prognosis prediction than other traditional clinical indicators. Functional assessment of the target genes of hsa-mir-139 and hsa-mir-5003 indicates that they were significantly enriched in multiple biological processes and pathways, including cell proliferation and cell migration regulation, pathways in cancer, and the cyclic adenosine monophosphate (cAMP) signaling pathway.ConclusionOur study indicates that the novel miRNA expression signature may be a potential prognostic biomarker for HCC patients.

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MicroRNA-101-2-5p targets the ApoB gene in the liver of chicken (Gallus Gallus)

Cited by 17 publications

References 36 publications

Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver

Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver

Transcriptome Profile Analysis Reveals an Estrogen Induced LncRNA Associated with Lipid Metabolism and Carcass Traits in Chickens (Gallus Gallus)

Identification of potential prognostic microRNA biomarkers for predicting survival in patients with hepatocellular carcinoma

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