microRNA-122 stimulates translation of hepatitis C virus RNA

Henke, Jura Inga; Goergen, Dagmar; Zheng, Jiangjun; Song, Yinglin; Schüttler, Christian G.; Fehr, Carmen; Jünemann, Christiane; Niepmann, Michael

doi:10.1038/emboj.2008.244

Cited by 598 publications

(553 citation statements)

References 42 publications

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“…Interestingly, expression of miR-122 has been shown to endow the ability of supporting efficient HCV RNA replication and infectious virion production in HepG2 cells (Narbus et al, 2011). Henke et al (2008) further uncovered a unique interaction of miR-122 with two binding sites in the 5′-UTR of the viral genome, which stimulates HCV translation by enhancing the association of ribosomes with the viral RNA. Structure analysis demonstrated that miR-122 triggers an open conformation of the HCV internal ribosome entry site (IRES), which may facilitate HCV translation (Díaz-Toledano et al, 2009).…”

Section: Mir-122 In Hcv Infectionmentioning

confidence: 99%

MiR-122 in hepatic function and liver diseases

et al. 2012

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Section: Mir-122 In Hcv Infectionmentioning

confidence: 99%

MiR-122 in hepatic function and liver diseases

et al. 2012

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“…Although miRNAs are generally known to down-regulate gene expression by inhibiting translation or inducing target mRNA degradation, a growing number of studies have demonstrated that miRNAs can also upregulate the expression of their targets under certain circumstances. 56,57 For example, miR-346 activates the activity of receptor-interacting protein 14 by increasing its protein expression 57 and miR-373 has been reported to target the promoter sequences of E-cadherin and cold-shock domaincontaining protein C2 to induce their expression. 58 Therefore, further experiments are required to determine whether any of these LNA-21-downregulated proteins are direct targets of miR-21.…”

Section: Silencing Of Pias3 Reverses the Effect Of Mir-21 Inhibition mentioning

confidence: 99%

Identification of Novel miR-21 Target Proteins in Multiple Myeloma Cells by Quantitative Proteomics

et al. 2012

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Substantial evidence indicates that microRNA-21 (miR-21) is a key oncomiR in carcinogenesis and is significantly elevated in multiple myeloma (MM). In this study, we explored the role of miR-21 in human MM cells and searched for miR-21 targets. By knocking down the expression of endogenous miR-21 in U266 myeloma cells, we observed reduced growth, an arrested cell cycle, and increased apoptosis. To further understand its molecular mechanism in the pathogenesis of MM, we employed a SILAC (stable isotope labeling by amino acids in cell culture)-based quantitative proteomic strategy to systematically identify potential targets of miR-21. In total, we found that the expression of 178 proteins was up-regulated significantly by miR-21 inhibition, implying that they could be potential targets of miR-21. Among these, the protein inhibitor of activated STAT3 (PIAS3) was confirmed as a direct miR-21 target by Western blotting and reporter gene assays. We further demonstrated that miR-21 enhances the STAT3-dependent signal pathway by inhibiting the function of PIAS3 and that down-regulation of PIAS3 contributes to the oncogenic function of miR-21. This elucidation of the role of PIAS3 in the miR-21-STAT3 positive regulatory loop not only may shed light on the molecular basis of the biological effects of miR-21 observed in MM cells but also has direct implications for the development of novel anti-MM therapeutic strategies.

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“…Some years ago, the central role of miRNAs in susceptibility to viral infection was described: miRNA-122 expression and hepatitis C virus infection (4), and miRNA-125b and HPV infection in cervical cancer (5).…”

Section: Introductionmentioning

confidence: 99%

microRNA Portraits in Human Vulvar Carcinoma

Maia

Lavorato-Rocha

Rodrigues

et al. 2013

Cancer Prevention Research

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Unregulated expression of microRNAs is well known and has already been demonstrated in many tumor types. However, in vulvar carcinoma this field has been unknown territory. Our study characterizes microRNA in vulvar tumors through an expression profile of 754 miRNAs, relating this with clinical and anatomopathologic data, and presence of HPV infection. Twenty HPV-negative and 20 HPV-positive samples, genotyped for high-risk HPVs (HPV16, 18, 31, 33) and a pool of seven normal vulvar skin samples were used for the identification of differentially expressed miRNAs by TLDA Quantitative Real Time PCR (qRT-PCR). Twenty-five differentially expressed microRNAs between HPV-positive and HPV-negative groups and 79 differentially expressed on the tumor compared with normal samples were obtained. A network between microRNA expression profiles and putative target mRNAs predicted by target prediction algorithms and previously demonstrated as relevant in vulvar carcinomas, such as TP53, RB, PTEN, and EGFR was constructed. Downregulation of both miR-223-5p and miR-19-b1-5p were correlated with the presence of lymph node metastasis; downregulation of miR-100-3p and miR-19-b1-5p were correlated with presence of vascular invasion; overexpression of miR-519b and miR-133a were associated with advanced FIGO staging. In conclusion, our study demonstrates that microRNAs may be clinically important in vulvar carcinomas and our findings may help for further studies on functional implications of miRNA deregulation in this type of cancer. Cancer Prev Res; 6(11); 1231-41. Ó2013 AACR.

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microRNA-122 stimulates translation of hepatitis C virus RNA

Cited by 598 publications

References 42 publications

MiR-122 in hepatic function and liver diseases

MiR-122 in hepatic function and liver diseases

Identification of Novel miR-21 Target Proteins in Multiple Myeloma Cells by Quantitative Proteomics

microRNA Portraits in Human Vulvar Carcinoma

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