MicroRNA-124 inhibits TNF-α- and IL-6-induced osteoclastogenesis

Ohnuma, Kenichiro; Kasagi, Shimpei; Uto, Kenichi; Noguchi, Yoriko; Nakamachi, Yuji; Saegusa, Jun

doi:10.1007/s00296-018-4218-7

Cited by 26 publications

(21 citation statements)

References 25 publications

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“…TNF-α and IL-6, increased during inflammatory responses, are reported to induce bone marrow macrophages to differentiate into osteoclast-like cells with a bone-resorptive activity in a RANKL (Receptor activator for nuclear factor κB ligand)-independent manner [41,42]. Compared to the placebo group, ELF-PEMF treatment did not affect TNF-α or IL-6 serum levels in our study cohort, suggesting that no inflammatory response occurred that could negatively affect bone quality.…”

Section: Discussionmentioning

confidence: 99%

Pulsed Electromagnetic Field Therapy Improves Osseous Consolidation after High Tibial Osteotomy in Elderly Patients—A Randomized, Placebo-Controlled, Double-Blind Trial

Ziegler

Nüssler

Wilbrand

et al. 2019

JCM

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Extremely low-frequency pulsed electromagnetic field (ELF-PEMF) therapy is proposed to support bone healing after injuries and surgical procedures, being of special interest for elderly patients. This study aimed at investigating the effect of a specific ELF-PEMF, recently identified to support osteoblast function in vitro, on bone healing after high tibial osteotomy (HTO). Patients who underwent HTO were randomized to ELF-PEMF or placebo treatment, both applied by optically identical external devices 7 min per day for 30 days following surgery. Osseous consolidation was evaluated by post-surgical X-rays (7 and 14 weeks). Serum markers were quantified by ELISA. Data were compared by a two-sided t-test (α = 0.05). Device readouts showed excellent therapy compliance. Baseline parameters, including age, sex, body mass index, wedge height and blood cell count, were comparable between both groups. X-rays revealed faster osseous consolidation for ELF-PEMF compared to placebo treatment, which was significant in patients ≥50 years (∆mean = 0.68%/week; p = 0.003). Findings are supported by post-surgically increased bone-specific alkaline phosphatase serum levels following ELF-PEMF, compared to placebo (∆mean = 2.2 µg/L; p = 0.029) treatment. Adverse device effects were not reported. ELF-PEMF treatment showed a tendency to accelerate osseous consolidation after HTO. This effect was stronger and more significant for patients ≥50 years. This ELF-PEMF treatment might represent a promising adjunct to conventional therapy supporting osseous consolidation in elderly patients. Level of Evidence: I.

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Section: Discussionmentioning

confidence: 99%

Pulsed Electromagnetic Field Therapy Improves Osseous Consolidation after High Tibial Osteotomy in Elderly Patients—A Randomized, Placebo-Controlled, Double-Blind Trial

Ziegler

Nüssler

Wilbrand

et al. 2019

JCM

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“…In addition, as a key cytokine in the pathophysiology of RA, TNF can stimulate osteoclast differentiation (Noack and Miossec, 2017). Anti-TNF drugs may improve the condition of patients with RA (Bek et al, 2017).…”

Section: Mirnas Regulate Other Factors In Osteoclastsmentioning

confidence: 99%

Roles of MicroRNAs in Bone Destruction of Rheumatoid Arthritis

Zhao

2020

Front. Cell Dev. Biol.

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As an important pathological result of rheumatoid arthritis (RA), bone destruction will lead to joint injury and dysfunction. The imbalance of bone metabolism caused by increased osteoclast activities and decreased osteoblast activities is the main cause of bone destruction in RA. MicroRNAs (MiRNAs) play an important role in regulating bone metabolic network. Recent studies have shown that miRNAs play indispensable roles in the occurrence and development of bone-related diseases including RA. In this paper, the role of miRNAs in regulating bone destruction of RA in recent years, especially the differentiation and activities of osteoclast and osteoblast, is reviewed. Our results will not only help provide ideas for further studies on miRNAs' roles in regulating bone destruction, but give candidate targets for miRNAs-based drugs research in bone destruction therapy of RA as well.

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“…Further function gain-and-loss experiments indicated that the increase in cell viability and the release of IL-6, IL-1β and TNF-α induced by 100 ng/mL LPS were significantly reversed by the miR-124. As reported before, miR-124 inhibits the proliferation of macrophages (Zhai et al 2020), and inhibits the expression of TNF-α, IL-6 and IL-1β (He et al 2019;Ohnuma et al 2019). In addition, miR-124 is essential for inducing and maintaining the M2 phenotype of macrophages (Veremeyko et al 2013).…”

Section: Discussionmentioning

confidence: 67%

Paeoniflorin reduces the inflammatory response of THP-1 cells by up‐regulating microRNA-124

Li¹,

Chen

Fan³

et al. 2021

Genes Genom

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Background The activation of macrophages and the release of inflammatory cytokines are the main reasons for the progress of systemic lupus erythematosus (SLE). MicroRNA (miRNA)-124 is involved in the regulation of macrophages and is a key regulator of inflammation and immunity. Objective To explore whether paeoniflorin (PF) regulates the biological functions of macrophages depends on miR-124. Methods RT-PCR, WB, ELISA, CCK-8 and flow cytometry were used to evaluate that PF regulated the biological functions of THP-1 cells through miR-124. Results PF significantly inhibited the proliferation while promotes the apoptosis of THP-1 cells, and inhibited the release of IL-6, TNF-α and IL-1βin THP-1 cells. RT-PCR results shown that PF up-regulated the expression of miR-124 in THP-1 cells. Functional recovery experiments showed that compared with the LPS + mimic-NC group, LPS + miR-124 mimic significantly inhibited the proliferation and the release of IL-6, TNF-α and IL-1β, but promoted the apoptosis of THP-1 cells. In addition, compared with the LPS + PF + inhibitor-NC group, LPS + PF + miR-124 inhibitor significantly promoted the proliferation and the release of IL-6, TNF-α and IL-1β, but inhibited the apoptosis of THP-1 cells. Conclusions By down-regulating miR-124, PF inhibits the proliferation and inflammation of THP-1 cells, and promotes the apoptosis of THP-1 cells.

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MicroRNA-124 inhibits TNF-α- and IL-6-induced osteoclastogenesis

Cited by 26 publications

References 25 publications

Pulsed Electromagnetic Field Therapy Improves Osseous Consolidation after High Tibial Osteotomy in Elderly Patients—A Randomized, Placebo-Controlled, Double-Blind Trial

Pulsed Electromagnetic Field Therapy Improves Osseous Consolidation after High Tibial Osteotomy in Elderly Patients—A Randomized, Placebo-Controlled, Double-Blind Trial

Roles of MicroRNAs in Bone Destruction of Rheumatoid Arthritis

Paeoniflorin reduces the inflammatory response of THP-1 cells by up‐regulating microRNA-124

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