MicroRNA 125 represses nonsense-mediated mRNA decay by regulating SMG1 expression

Wang, Gang; Jiang, Bowen; Jia, Chen-Liang; Chai, Baofeng; Liang, Aihua

doi:10.1016/j.bbrc.2013.03.129

Cited by 21 publications

(17 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). One of them, dre-miR 125a-2, belongs to the miR 125 family, which is highly conserved in eukaryotes39 and is involved in many different cellular processes, including cell proliferation4041, differentiation and apoptosis, through the targeting of different transcription factors40, matrix-metalloproteases4243, growth factors44 and nonsense-mediated mRNA decay pathway45. In addition, miR-125 controls the differentiation of immune cells, thereby affecting responses to bacterial and viral infection3946474849.…”

Section: Resultsmentioning

confidence: 99%

Extracellular vesicle-mediated export of fungal RNA

Silva

Puccia

Rodrigues

et al. 2015

Sci Rep

185

213

View full text Add to dashboard Cite

Extracellular vesicles (EVs) play an important role in the biology of various organisms, including fungi, in which they are required for the trafficking of molecules across the cell wall. Fungal EVs contain a complex combination of macromolecules, including proteins, lipids and glycans. In this work, we aimed to describe and characterize RNA in EV preparations from the human pathogens Cryptococcus neoformans, Paracoccidiodes brasiliensis and Candida albicans, and from the model yeast Saccharomyces cerevisiae. The EV RNA content consisted mostly of molecules less than 250 nt long and the reads obtained aligned with intergenic and intronic regions or specific positions within the mRNA. We identified 114 ncRNAs, among them, six small nucleolar (snoRNA), two small nuclear (snRNA), two ribosomal (rRNA) and one transfer (tRNA) common to all the species considered, together with 20 sequences with features consistent with miRNAs. We also observed some copurified mRNAs, as suggested by reads covering entire transcripts, including those involved in vesicle-mediated transport and metabolic pathways. We characterized for the first time RNA molecules present in EVs produced by fungi. Our results suggest that RNA-containing vesicles may be determinant for various biological processes, including cell communication and pathogenesis.

show abstract

Section: Resultsmentioning

confidence: 99%

Extracellular vesicle-mediated export of fungal RNA

Silva

Puccia

Rodrigues

et al. 2015

Sci Rep

185

213

View full text Add to dashboard Cite

show abstract

“…In addition, both these miRNAs are primarily expressed in the nervous system and there is evidence that both regulate several events in neurons (Sun et al, 2013; Krichevsky et al, 2006). Another miRNA that may contribute to this regulation is miR-125, a neurally expressed miRNA that was recently shown to repress the expression of the NMD factor SMG1 (Wang et al, 2013) and promote the early neural specification of human embryonic stem cells (Boissart et al, 2012). Thus, there is growing constellation of miRNAs that are candidates to collaborate with miR-128 to repress NMD in neural precursor cells and thereby drive their differentiation.…”

Section: Discussionmentioning

confidence: 99%

Posttranscriptional Control of the Stem Cell and Neurogenic Programs by the Nonsense-Mediated RNA Decay Pathway

et al. 2014

View full text Add to dashboard Cite

SUMMARY The mechanisms dictating whether a cell proliferates or differentiates have undergone intense scrutiny but remain poorly understood. Here, we report that a central component in the nonsense-mediated RNA decay (NMD) pathway—UPF1—plays a key role in this decision by promoting the proliferative, undifferentiated cell state. UPF1 acts, in part, by destabilizing the NMD substrate encoding the TGFβ inhibitor, SMAD7, and stimulating TGFβ signaling. UPF1 also promotes the decay of mRNAs encoding many other proteins that oppose the proliferative, undifferentiated cell state. Neural differentiation is triggered when NMD is downregulated by neurally expressed microRNAs (miRNAs). This UPF1-miRNA circuitry is highly conserved and harbors negative feedback loops that act as a molecular switch. Our results suggest that the NMD RNA decay pathway collaborates with the TGF-β signaling pathway to lock-in the stem-like state, a cellular state that is stably reversed when neural differentiation signals that induce NMD-repressive miRNAs are received.

show abstract

“…In summary, NMD activity is modulated in a number of different ways to affect specific physiological or pathological outcomes (Figure ). Some of the mechanisms that lead to NMD inhibition include downregulation of NMD factors by microRNAs (Jin, Zhang, Ma, & Ren, ; Karam & Wilkinson, ; Wang, Jiang, Jia, Chai, & Liang, ) or proteasomal degradation (Feng et al, ; Jagannathan et al, ), cleavage of specific NMD factors to produce dominant negative products (Popp & Maquat, ), competition between alternative decay pathways (Gong et al, ), translation inhibition (Li et al, ), and activation of signaling pathways such as p38 MAPK (Nickless et al, ). In physiological scenarios, NMD inhibition leads to the increased abundance of one or more physiological NMD targets that confer a benefit to the cell.…”

Section: Context‐specific Modifiers Of Nmd Efficiencymentioning

confidence: 99%

How to get away with nonsense: Mechanisms and consequences of escape from nonsense‐mediated RNA decay

et al. 2019

View full text Add to dashboard Cite

Nonsense-mediated RNA decay (NMD) is an evolutionarily conserved RNA quality control process that serves both as a mechanism to eliminate aberrant transcripts carrying premature stop codons, and to regulate expression of some normal transcripts. For a quality control process, NMD exhibits surprising variability in its efficiency across transcripts, cells, tissues, and individuals in both physiological and pathological contexts. Whether an aberrant RNA is spared or degraded, and by what mechanism, could determine the phenotypic outcome of a disease-causing mutation. Hence, understanding the variability in NMD is not only important for clinical interpretation of genetic variants but also may provide clues to identify novel therapeutic approaches to counter genetic disorders caused by nonsense mutations. Here, we discuss the current knowledge of NMD variability and the mechanisms that allow certain transcripts to escape NMD despite the presence of NMD-inducing features.This article is categorized under:RNA Turnover and Surveillance > Turnover/Surveillance

show abstract

MicroRNA 125 represses nonsense-mediated mRNA decay by regulating SMG1 expression

Cited by 21 publications

References 16 publications

Extracellular vesicle-mediated export of fungal RNA

Extracellular vesicle-mediated export of fungal RNA

Posttranscriptional Control of the Stem Cell and Neurogenic Programs by the Nonsense-Mediated RNA Decay Pathway

How to get away with nonsense: Mechanisms and consequences of escape from nonsense‐mediated RNA decay

Contact Info

Product

Resources

About