MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A

Liu, Meiyue; Zhang, Yue; Zhang, Jie; Cai, Haifeng; Zhang, Chao; Zhao, Yupei; Niu, Yi; Wang, Huan; Wei, Xiaomei; Wang, Wei; Gao, Peng; Li, Hongmin; Zhang, Jinghua; Sun, Guogui

doi:10.1038/s41419-017-0218-x

Cited by 65 publications

(52 citation statements)

References 53 publications

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“…In addition, miR-1253 and RAB3D have been found to be participated in the development of human malignancies. 14,15 Thus, by investigating the effects of hsa_circ_0087862 on miR-1253/RAB3D axis, this paper further explored the potential molecular mechanisms of hsa_circ_0087862 affecting NSCLC development. As far as we know, this was the first time that hsa_circ_0087862 has been studied in detail in NSCLC.…”

Section: Introductionmentioning

confidence: 99%

<p>CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis</p>

Wan

Xiong

et al. 2020

OTT

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Purpose: Circular RNAs (circRNAs) have been found to regulate several human tumors. The present study was to explore the mechanism of hsa_circ_0087862 in regulating nonsmall cell lung cancer (NSCLC). Methods: Totally 102 NSCLC cases were enrolled. NCI-H1359 and A549 cells were transfected. Cells viability, apoptosis, migration and invasion were determined by CCK-8 assay, flow cytometry, scratch test and transwell experiment, respectively. Luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay were performed. Xenograft tumor experiments were performed using nude mice. hsa_circ_0087862, miR-1253 and RAB3D expression in tissues/cells were detected by qRT-PCR. RAB3D and Ki67 protein expressions in cells/tissues were researched by Western blot and immunohistochemistry. Apoptosis of xenograft tumor tissue cells was detected using Tunel assay. Results: hsa_circ_0087862 was significantly up-regulated in NSCLC patients, which was associated with poor prognosis (P < 0.05). hsa_circ_0087862 down-regulation prominently weakened NSCLC cells viability, migration, invasion and enhanced apoptosis (P < 0.01). hsa_circ_0087862 overexpression exhibited the opposite results in NSCLC cells. miR-1253 was sponged by hsa_circ_0087862. miR-1253 expression in NSCLC tissues was negatively correlated with hsa_circ_0087862 (P < 0.001). RAB3D expression in NSCLC was directly inhibited by miR-1253. miR-1253 down-regulation or RAB3D overexpression dramatically reversed NSCLC cells phenotype induced by hsa_circ_0087862 down-regulation. hsa_circ_0087862 down-regulation markedly inhibited tumor growth in vivo (P < 0.01). In xenograft tumor tissues, hsa_circ_0087862 down-regulation obviously decreased expression of RAB3D, Ki67 and increased apoptosis. Conclusion: hsa_circ_0087862 acted as an oncogene in NSCLC by targeting miR-1253/ RAB3D.

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Section: Introductionmentioning

confidence: 99%

<p>CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis</p>

Wan

Xiong

et al. 2020

OTT

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“…Subsequently, more and more studies which focusing on the miR-24-3p expression levels with cancer progression, metastasis and prognosis of patients were carried on [4][5][6][7][14][15][16]. Thus, the exact role of miR-24-3p on the clinical prognosis of patients in various human carcinomas still need to further investigate.…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNAs (miRNAs), a kind of endogenous non-coding RNAs of 18-22 nucleotides in length, negatively regulate target genes expression at posttranscriptional level [1][2][3]. As either oncogenes or anti-oncogenes, they are found to play vital roles in a wide range of fundamental biological processes, such as proliferation [4,5], differentiation [6,7], apoptosis [8,9], cell cycle [10][11][12][13], metastasis [14][15][16], stress response [17][18][19], metabolic [20][21][22] and etc. Owing to its detectability and stability in tissues, marrow or blood, a growing number of studies suggests that miRNAs can serve as promising biomarkers for the prognosis of carcinomas [23].…”

Section: Introductionmentioning

confidence: 99%

MiR-24-3p and various cancers: From a meta-analysis view

Wang

Chen

Zhang

et al. 2020

Preprint

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Background: A growing number of researches suggests that microRNAs (miRNAs) as oncogene or tumor suppressor genes play a fundamental role in various kinds of cancers. Among them, miR-24-3p, as a star molecule, is widely studied. However, the prognostic value of miR-24-3p is unclear and controversial. We conducted this meta-analysis to evaluate the prognostic value of miR-24-3p in a variety of cancers by integrated existing articles from four databasesMethods: PubMed, Embase, Web of Science, and Cochrane Library (last update in March 2020) were searched for approach literature. Hazard ratios (HRs) and odds ratios (ORs) were used to evaluate the association between miR-24-3p expression levels and prognostic value or clinicopathological characteristics, respectively.Results: A total of 15 studies from 14 literature were finally qualified and concluded in the present meta-analysis. A significantly worse overall survival was observed in higher expression of miR-24-3p cancer group for OS(Overall survival) of log rank tests and cox multivariate regression by fixed effects model. Also, we found a significant correlation between elevated miR-24-3p levels to RFS (Recurrence-free survival) and DFS(Disease free survival). In addition, the pooled odds ratios (ORs) showed that evaluated miR-24-3p was also associated with the larger tumor size (≥5cm) and advanced TNM stage (Ⅲ and Ⅳ).Conclusion: Built on the above findings, elevated expression levels of miR-24-3p may serve as a promising biomarker used to predict the worse prognosis of cancer patients.

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“…It was known the role of Wnt5a-S protein in the processes of tumor formation and growth. Liu et al positioned WNT5A as a target for miR-1253 [76]. In vitro and in vivo experiments showed that overexpression of miR-1253 significantly inhibited proliferation, migration, and invasion of NSCLC cells, while inhibition of miR-1253 had the opposite effect.…”

Section: Epigenetic Factors In the Development Of Cancermentioning

confidence: 99%

Radon Biomonitoring and microRNA in Lung Cancer

Bersimbaev

Pulliero

Bulgakova

et al. 2020

IJMS

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Radon is the number one cause of lung cancer in non-smokers. microRNA expression in human bronchial epithelium cells is altered by radon, with particular reference to upregulation of miR-16, miR-15, miR-23, miR-19, miR-125, and downregulation of let-7, miR-194, miR-373, miR-124, miR-146, miR-369, and miR-652. These alterations alter cell cycle, oxidative stress, inflammation, oncogene suppression, and malignant transformation. Also DNA methylation is altered as a consequence of miR-29 modification induced by radon. Indeed miR-29 targets DNA methyltransferases causing inhibition of CpG sites methylation. Massive microRNA dysregulation occurs in the lung due to radon expose and is functionally related with the resulting lung damage. However, in humans this massive lung microRNA alterations only barely reflect onto blood microRNAs. Indeed, blood miR-19 was not found altered in radon-exposed subjects. Thus, microRNAs are massively dysregulated in experimental models of radon lung carcinogenesis. In humans these events are initially adaptive being aimed at inhibiting neoplastic transformation. Only in case of long-term exposure to radon, microRNA alterations lead towards cancer development. Accordingly, it is difficult in human to establish a microRNA signature reflecting radon exposure. Additional studies are required to understand the role of microRNAs in pathogenesis of radon-induced lung cancer.

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MicroRNA-1253 suppresses cell proliferation and invasion of non-small-cell lung carcinoma by targeting WNT5A

Cited by 65 publications

References 53 publications

<p>CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis</p>

<p>CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis</p>

MiR-24-3p and various cancers: From a meta-analysis view

Radon Biomonitoring and microRNA in Lung Cancer

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