MicroRNA-125a-5p partly regulates the inflammatory response, lipid uptake, and ORP9 expression in oxLDL-stimulated monocyte/macrophages

Chen, Ting; Huang, Zhouqing; Wang, Liansheng; Wang, Yue; Wu, Feizhen; Shao, Meng; Wang, Changqian

doi:10.1093/cvr/cvp121

Cited by 280 publications

(234 citation statements)

References 43 publications

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“…The present study also found that miR-146b-5p may be induced by oxLDL stimulation in a time-and dose-dependent manner, which was consistent with the results of a previous study (13).…”

Section: Discussionsupporting

confidence: 93%

“…A previous study demonstrated upregulation of miR-146b-5p in oxidized low-density lipoprotein (oxLDL)-stimulated monocytes (13); however, to the best of our knowledge, no further study on the role of miR-146b-5p in AS has been performed. Thus, the present study investigated the potential role of miR-146b-5p in AS and explored the underlying molecular mechanisms.…”

Section: Introductionmentioning

confidence: 86%

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Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6

Lin

2017

Exp Ther Med

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Abstract. Atherosclerosis (AS) is a chronic inflammation in response to lipid accumulation. Increasing evidence has demonstrated that numerous microRNAs (miRs) have critical roles in inflammatory responses. A previous study suggested that miR-146b-5p is possibly associated with AS; however, its exact role has remained largely elusive. The present study aimed to investigate the potential role of miR-146b-5p in AS and to explore the underlying mechanism. Fist, the levels of miR-146b-5p were determined in foam cells and clinical specimens from patients with AS by reverse-transcription quantitative PCR. The role of miR-146b-5p in AS was then investigated by using miR-146b-5p inhibitor. The results demonstrated that the expression levels of miR-146b-5p were elevated in the lesions of patients with AS. In addition, the levels of miR-146b-5p in THP-1 cells stimulated with phorbol 12-myristate 13-acetate (100 nM) to induce their differentiation into macrophages were dose-and time-dependently elevated by oxidized low-density lipoprotein treatment applied for inducing foam cell formation. miR-146b-5p was also revealed to directly target tumor necrosis factor receptor-associated factor 6 (TRAF6), which functions as a signal transducer in the nuclear factor-κB (NF-κB) pathway. Furthermore, the present study reported for the first time that miR-146b-5p inhibition promotes the inflammatory response and enhances lipid uptake during foam cell formation. In conclusion, miR-146b-5p inhibition promoted chronic inflammation and had a detrimental role during AS-associated foam cell formation by targeting TRAF6.

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“…The present study also found that miR-146b-5p may be induced by oxLDL stimulation in a time-and dose-dependent manner, which was consistent with the results of a previous study (13).…”

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 86%

Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6

Lin

2017

Exp Ther Med

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“…With the exception of miR-193b, the amplitude of change in the abundance of individual guide strands was less than 2-fold (14). In contrast to the small changes in miRNA expression described in our previous study, several studies have described robust induction of a subset of miRNAs in human monocytes responding to LPS and other inflammatory treatments (15)(16)(17)(18)(19). These observations led us to the important discovery that all activation-associated miRNAs tested had higher basal expression levels in MDMs than in monocytes (14).…”

mentioning

confidence: 62%

Regulation of Activation-associated MicroRNA Accumulation Rates during Monocyte-to-macrophage Differentiation

Eigsti

Sudan

Wilson

et al. 2014

Journal of Biological Chemistry

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Background: A set of miRNAs accumulates in polarized macrophages. Results: The same miRNAs accrued during macrophage differentiation with varied rates depending on exogenous conditions and were regulated at a preprocessing step. Conclusion: Key monocytic cell miRNAs accumulated in response to activation and differentiation signals. Significance: Accumulation of key miRNAs was tailored to diverse stimuli leading to customized miRNA expression.

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“…Our previous studies [24] have shown that some miRNAs are upregulated in oxLDL-stimulated monocytes/macrophages. In this study, the expression of miR-29a is notably upregulated in Table 1 Comparison of inflammatory biomarkers secreted by transfecting the miR-29a inhibitor and mimics and siLPL.…”

Section: Discussionmentioning

confidence: 95%

MicroRNA-29a regulates pro-inflammatory cytokine secretion and scavenger receptor expression by targeting LPL in oxLDL-stimulated dendritic cells

Chen

et al. 2011

FEBS Letters

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Edited by Tamas Dalmay Keywords:MicroRNA-29a Oxidized low density lipoprotein Dendritic cell Lipoprotein lipase Immuno-inflammation a b s t r a c t There is increasing evidence that microRNAs (miRNAs) play important roles in cell proliferation, apoptosis and differentiation that accompany inflammatory responses. However, whether microRNAs are associated with DC immuno-inflammatory responses with oxidized low density lipoprotein (oxLDL) stimulation is not yet known. Our study aims to explore the link of miRNAs with lipidoverload and immuno-inflammatory mechanism for atherosclerosis. In DCs transfected with microRNA-29a mimics or inhibitors, we showed that microRNA-29a plays an important role in proinflammatory cytokine secretion and scavenger receptor expression upon oxLDL-treatment. Furthermore, we suggest an additional explanation for the mechanism of microRNA-29a regulation of its functional target, lipoprotein lipase. We conclude that microRNA-29a could regulate proinflammatory cytokine secretion and scavenger receptor expression by targeting lipoprotein lipase in oxLDL-stimulated dendritic cells.

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MicroRNA-125a-5p partly regulates the inflammatory response, lipid uptake, and ORP9 expression in oxLDL-stimulated monocyte/macrophages

Abstract: MicroRNA-125a-5p may partly provide post-transcriptional regulation of the proinflammatory response, lipid uptake, and expression of ORP9 in oxLDL-stimulated monocyte/macrophages.

Cited by 280 publications

References 43 publications

Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6

Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6

Regulation of Activation-associated MicroRNA Accumulation Rates during Monocyte-to-macrophage Differentiation

MicroRNA-29a regulates pro-inflammatory cytokine secretion and scavenger receptor expression by targeting LPL in oxLDL-stimulated dendritic cells

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