MicroRNA‐127 Inhibits the Progression of Melanoma by Downregulating Delta‐Like Homologue 1

Abstract

Objective. Melanoma is the most common form of skin cancer with low survival rate and poor prognosis. MicroRNAs (miRNAs) have been reported to play essential roles in progression of melanoma. However, the role and mechanism of miR-127 in the process of melanoma remain poorly understood. Methods. The expressions of miR-127 and delta-like homologue 1 (DLK1) were measured in melanoma tissues and cells by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Cell proliferation and apoptosis were measured by MTT assay, flow cytometry, and Western blot. The interaction between miR-127 and DLK1 was investigated by bioinformatics analysis, luciferase activity assay, and RNA immunoprecipitation (RIP). Murine xenograft model was conducted to investigate the effect of miR-127 on tumor growth in vivo. Results. miR-127 was inhibited and DLK1 mRNA was enhanced in melanoma tissues and cells. Low abundance of miR-127 in melanoma tissues predicted a poor prognosis and was associated with the malignant clinicopathological features. Overexpression of miR-127 inhibited cell proliferation and induced apoptosis in melanoma cells. Moreover, DLK1 was targeted by miR-127 and its restoration reversed the regulatory effect of miR-127 on the process of melanoma. Besides, the addition of miR-127 suppressed xenograft tumor growth via suppressing DLK1 protein level in nude mice. Conclusion. miR-127 blocked the development of melanoma by targeting DLK1, providing a novel biomarker for the treatment of melanoma.

MicroRNA Profile for Diagnostic and Prognostic Biomarkers in Thyroid Cancer

“…In contrast, miR-127 has also been reported to act as a tumor suppressor in various tumor types. The target genes downregulated by miR-127 have been shown as follows: B-cell lymphoma 6 (BCL6) in breast cancer [41], replication initiator 1 (REPIN1) in glioma [42], formin-like 3 (FMNL3) in esophageal cancer [43], cytochrome C oxidase assembly factor 1 homologue (COA1) in giant cell tumor of bone [44], and delta like non-canonical notch ligand 1 (DLK1) in melanoma [45].…”

Circulating miRNAs Expression in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

“…IL-10, an important anti-inflammatory cytokine that suppresses Th 1 -related responses, has been consistently observed to be significantly reduced in the cerebrospinal fluid of ME/CFS patients in comparison to the controls [ 44 ]. In addition, miR-127 upregulation has been reported to inhibit cell proliferation and induce apoptosis [ 31 , 45 ].…”

Role of MicroRNAs and Long Non-Coding RNAs in Sarcopenia

“…The enforced expression of miR-127 in a mouse model of muscular dystrophy ameliorated muscle disease [ 111 ]. In addition, miR-127 not only inhibits myoblast proliferation, but also functions as a tumor suppressor in several types of cancer [ 112 , 113 , 114 , 115 ]. This implies that miR-127 functions as an activator of cell differentiation and a suppressor of cell proliferation.…”