MicroRNA-127 is a tumor suppressor in human esophageal squamous cell carcinoma through the regulation of oncogene FMNL3

Gao, Xu-Hui; Wang, Xuelian; Cai, Kaican; Wang, Wujun; Ju, Qun; Yang, Xiyao; Wang, Haofei; Wu, Hua

doi:10.1016/j.ejphar.2016.09.025

Cited by 25 publications

(20 citation statements)

References 21 publications

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“…FMNL3 is a newly discovered gene whose encoded product belongs to the DRFs family . Some studies showed that FMNL3 is highly expressed in a variety of malignant tumors and is associated with poor prognosis . Recent studies have also shown that members of the DRFs family induce pseudopodia by regulating the cytoskeletal network based on actin and tubulin, which promotes the migration and invasion of cancer cells .…”

Section: Discussionmentioning

confidence: 99%

“…Because FMNL3 overexpression is associated with poor prognosis of tongue cancer and it has been shown to promote the invasion and metastasis of cancer cells in a variety of malignancies, we further hypothesized that FMNL3 may influence tongue cancer metastasis. Metastasis is a complex process that requires tumor cells to transfer the cytoskeleton from the original tissue to a new focal point .…”

Section: Discussionmentioning

confidence: 99%

“…As one of the important members of the DRFs subgroup, there are also reports that FMNL3 participates in cell‐cell adhesion and promotes cell migration, promoting the invasion and metastasis of various malignant tumors . FMNL3 has recently been shown to be overexpressed in some malignancies, including colorectal cancer, nasopharyngeal carcinoma, and esophageal cancer . However, little research has been reported on the expression levels and effects of FMNL3 in TSCC.…”

Section: Introductionmentioning

confidence: 99%

“…11 FMNL3 has recently been shown to be overexpressed in some malignancies, including colorectal cancer, 12 nasopharyngeal carcinoma, 13 and esophageal cancer. 14 However, little research has been reported on the expression levels and effects of FMNL3 in TSCC.…”

Section: Introductionmentioning

confidence: 99%

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High expression of FMNL3 associates with cancer cell migration, invasion, and unfavorable prognosis in tongue squamous cell carcinoma

Liu

Chen

Yang

et al. 2019

J Oral Pathology Medicine

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Background The aim of this study was to ascertain the expression and role of FMNL3 in tongue squamous cell carcinoma (TSCC) tissues. Materials and Methods Immunohistochemistry, qRT‐PCR, and western blot were used to study the expression of FMNL3 in TSCC tissues and its adjacent normal tissues. The relationship between FMNL3 overexpression and various clinicopathological parameters and patients’ survival were further assessed. Next, we applied SCC25 and UM1 cell lines to study the effect of FMNL3 on tumor cell proliferation, migration, and invasion by silencing FMNL3. Results In this study, we demonstrated that FMNL3 expression was notably upregulated in TSCC tissues and associated with poor prognosis. The Cox regression survival analyses identified FMNL3 as an important independent predictor for patients’ overall survival. We found that FMNL3 silencing by siRNA inhibited SCC25 and UM1 cell migration an invasion. Conclusion FMNL3 overexpression is related to the metastasis of TSCC and poor prognosis. High expression of FMNL3 may become a new potential prognostic biomarker for patients with TSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High expression of FMNL3 associates with cancer cell migration, invasion, and unfavorable prognosis in tongue squamous cell carcinoma

Liu

Chen

Yang

et al. 2019

J Oral Pathology Medicine

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“…Currently, numerous studies have shown that miRNAs play an important role in the carcinogenesis, progression, and prognosis of ESCC. miR-127, miR-98, miR-145, and miR-153 were reported to be aberrantly downregulated, and miR-1288, miR-183, and miR-17-92 were upregulated in ESCC 11–17. Moreover, Meng et al showed that overexpression of miR-202 promoted proliferation and migration of human ESCC by inhibiting laminin alpha 1-mediated FAK-PI3K-Akt signaling 18.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-381 enhances radiosensitivity in esophageal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis protein

Zhou¹,

Cui²,

Yu³

et al. 2017

OTT

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BackgroundIncreasing evidence indicates that radioresistance remains a major problem in the treatment of patients with esophageal squamous cell carcinoma (ESCC). This study was designed to investigate the expression of microRNA-381 (miR-381) and its function in the radioresistance of ESCC.MethodsIn this study, miR-381 expression was first detected in ESCC cell lines and tissue samples by quantitative real-time polymerase chain reaction (qRT-PCR). Then, the effects of miR-381 expression on growth, apoptosis, and radiosensitivity of ESCC cells were analyzed by MTT, colony formation, and flow cytometry, respectively. Dual-luciferase reporter assays were performed to validate the regulation of a putative target of miR-381, in corroboration with qRT-PCR and Western blotting assays.ResultsESCC cell lines or tissues were found to express significantly lower miR-381 than normal esophageal epithelial cells or adjacent normal tissues, respectively. Ectopic expression of miR-381 in ESCC cell lines blocked proliferation, reduced colony formation, enhanced apoptosis, and increased radiosensitivity by enhancing irradiation-induced apoptosis. In addition, dual-luciferase reporter assays showed that miR-381 binds to the 3′-untranslated region of X-linked inhibitor of apoptosis protein (XIAP), suggesting that XIAP should be a direct target of miR-381. Re-expression of miR-381 suppressed XIAP protein expression in ESCC cells, and the effects of miR-381 upregulation on ESCC cells were found to be similar with silencing of XIAP. In addition, XIAP mRNA expression significantly increased in ESCC tissues and was inversely correlated with miR-381 expression.ConclusionThe results of this study suggest that miR-381/XIAP pathway contributed to the growth inhibition, increase in apoptosis, and enhancement of radiosensitivity in ESCC cells Therefore, miR-381 may be a potential therapeutic target in human ESCC.

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The mechanism study of miR‐125b in occurrence and progression of multiple myeloma

Gao

Xiao

et al. 2017

Cancer Medicine

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Although many efforts have contributed to improve our knowledge of molecular pathogenesis about multiple myeloma (MM), the role and significance of microRNAs and long noncoding RNAs in MM cells, along with the core mechanism remains virtually absent. The mRNA levels of miR‐125b and MALAT1 in MM cell lines were detected by qRT‐PCR. The influence of Lenti‐Sh‐miR‐125b on cell viability and the Notch‐1 pathway‐related proteins were assessed by MTT method and western blot, respectively. We also investigated the regulation effect between MALAT1 and Notch1 pathway. Moreover, the connection between Notch1 signaling and MM cell growth was discussed in‐depth. The reverse effect of pcDNA‐Notch1 on the cell viability and Notch‐1 pathway proteins induced by Si‐MALAT1 was also studied. Furthermore, miR‐125b overexpressing MM cell lines were injected subcutaneously into nude mice. MiR‐125b and MALAT1 were inversely expressed in MM cell lines. Lenti‐Sh‐miR‐125b inhibited the expression of MALAT1 and Notch‐1 protein. Binding sites were confirmed between miR‐125b and MALAT1, and silencing MALAT1 did not alter the expression of Notch‐1. The apoptosis rate was increased and the survival rate was decreased obviously in GSI XII (targeted cleavage of Notch‐1 receptor) group, along with the inhibited Notch1 and HES1 proteins. Moreover, the decreased cell viability and Notch‐1 pathway proteins induced by Si‐MALAT1 could be reversed by pcDNA‐Notch1. Lenti‐Sh‐miR‐125b promoted survival and decreased Notch1 and HES1 proteins levels, while this effect was reversed by si ‐MALAT1. MiR‐125b regulated MALAT1 expression via Notch1 signaling pathway to regulate cell growth, thus participating in the occurrence and progression of MM, which functioned as a therapeutic target for tracking MM.

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MicroRNA-127 is a tumor suppressor in human esophageal squamous cell carcinoma through the regulation of oncogene FMNL3

Cited by 25 publications

References 21 publications

High expression of FMNL3 associates with cancer cell migration, invasion, and unfavorable prognosis in tongue squamous cell carcinoma

High expression of FMNL3 associates with cancer cell migration, invasion, and unfavorable prognosis in tongue squamous cell carcinoma

MicroRNA-381 enhances radiosensitivity in esophageal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis protein

The mechanism study of miR‐125b in occurrence and progression of multiple myeloma

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