microRNA-1271-5p/TIAM1 suppresses the progression of ovarian cancer through inactivating Notch signaling pathway

Abstract

Objective Ovarian cancer (OC) has been regarded as the most malignant gynecological neoplasm and often confers grave outcomes owing to the frequent metastasis and high recurrence. A previous study has demonstrated that miR-1271-5p is implicated in OC progression, however, the possible mechanism of it remains unknown. The purpose of this investigation was to explore how miR-1271-5p regulates the progression of OC. Methods Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases were employed to analyze the differentially expressed miRNAs or genes as well as their corresponding prognostic values. miR-1271-5p expression in OC cells was examined by qRT-PCR. Cell counting kit 8 (CCK-8), colony formation, and transwell tests were conducted to evaluate the proliferation, migration and invasion potentials. Bioinformatics prediction and luciferase activity analysis were utilized to predict and verify the target gene of miR-1271-5p. Western blot assay was carried out to measure protein expression. Results miR-1271-5p was significantly decreased in OC and its down-regulation was associated with the grave outcome of OC patients. Upregulation of miR-1271-5p inhibited cell viability, but miR-1271-5p knockdown promoted the proliferation of OC cells. TIAM1 was a direct target gene of miR-1271-5p and expressed in OC tissues at higher level. High expression of TIAM1 induced the poorer prognosis of patients with OC. Further functional analyses showed that the suppressive role of miR-1271-5p on OC cell malignant behaviors was overturned by the upregulation of TIAM1. The protein levels of Cyclin D1, HES1, NOTCH and NUMB were remarkably changed due to the abnormal expression of miR-1271-5p and TIAM1. Conclusion To sum up, miR-1271-5p inhibits proliferation, invasion and migration of OC cells by directly repressing TIAM1 to inactivate the Notch signaling pathway, which provides an alternative therapeutic candidate for the advancement of OC treatment.

Identification of Novel Prognostic Signatures for Clear Cell Renal Cell Carcinoma Based on ceRNA Network Construction and Immune Infiltration Analysis

“…Previous studies have suggested that miR-1271-5p is closely associated with the progression of several malignancies. Han et al found that miR-1271-5p suppressed the progression of ovarian cancer through Notch signaling [ 29 ]. Furthermore, it was reported that MALAT1 sponges miR-1271-5p and facilitates multiple myeloma and ovarian cancer progression by regulating SOX13 and E2F5, respectively [ 30 , 31 ].…”

“…Moreover, MALAT1 has been reported to modify ccRCC progression via regulating miR-194-5p/ACVR2B signaling [28]. Previous studies have suggested that miR-1271-5p is closely associated with the progression of suppressed the progression of ovarian cancer through Notch signaling [29]. Furthermore, it was reported that MALAT1 sponges miR-1271-5p and facilitates multiple myeloma and ovarian cancer progression by regulating SOX13 and E2F5, respectively [30,31].…”

Overview of the signaling pathways involved in metastasis: An intriguing story-tale of the metastatic journey of ovarian cancer cells

“…miR-1271-5p knockdown promoted the proliferative ability of ovarian cancer cells. Levels of HES1 and NOTCH were noted to be reduced in miR-1271-5p-expressing ovarian cancer cells (46).…”

“…TIAM1 (T cell lymphoma invasion and metastasis-1) promoted NOTCH pathway and consequently enhanced invasion of ovarian cancer cells (46). miR-1271-5p acted as a tumor suppressor and targeted TIAM1.…”

The Targeting of Noncoding RNAs by Quercetin in Cancer Prevention and Therapy

“…miR-223 and miR-1275 promoted cancer occurrence and development by activating the Notch signaling pathway [ 64 , 65 ]. miR-1271-5p and miR-34 suppressed the proliferation, invasion, and migration of cancer cells by blocking the Notch signaling cascade [ 66 , 67 ]. miR-139-5p and miR-199b-5p could sensitize cancer cells to chemotherapeutic agents by reducing the activity of the Notch1 signaling [ 68 , 69 ].…”