MicroRNA-129-5p inhibits C2C12 myogenesis and represses slow fiber gene expression in vitro

Peng, Ying; Xu, Meixue; Dou, Mingle; Shi, Xin’e; Yang, Gongshe; Li, Xiao

doi:10.1152/ajpcell.00578.2020

Cited by 10 publications

(8 citation statements)

References 43 publications

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“…Mechanistically, Gm16062 sponges miR-129-2-3p, thereby liberating the inhibitory effect of miR-129-2-3p on Mef2a to up-regulate the expression of Myod1 and Myog. It is noteworthy that miR-129-5p, in the same family as miR-129-2-3p, inhibits C2C12 myogenesis by targeting Mef2a [44]. Bioinformatics analysis revealed that Mef2a is also the target gene of miR-129-2-3p.…”

Section: Discussionmentioning

confidence: 97%

Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice

Yang,

Zhang,

Liu

et al. 2024

IJMS

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The normal growth and development of skeletal muscle is essential for the health of the body. The regulation of skeletal muscle by intestinal microorganisms and their metabolites has been continuously demonstrated. Acetate is the predominant short-chain fatty acids synthesized by gut microbiota through the fermentation of dietary fiber; however, the underlying molecular mechanisms governing the interaction between acetate and skeletal muscle during the rapid growth stage remains to be further elucidated. Herein, specific pathogen-free (SPF) mice, germ-free (GF) mice, and germ-free mice supplemented with sodium acetate (GS) were used to evaluate the effects of acetate on the skeletal muscle growth and development of young mice with gut microbiota deficiency. We found that the concentration of serum acetate, body mass gain, succinate dehydrogenase activity, and expression of the myogenesis maker gene of skeletal muscle in the GS group were higher than those in the GF group, following sodium acetate supplementation. Furthermore, the transcriptome analysis revealed that acetate activated the biological processes that regulate skeletal muscle growth and development in the GF group, which are otherwise inhibited due to a gut microbiota deficiency. The in vitro experiment showed that acetate up-regulated Gm16062 to promote skeletal muscle cell differentiation. Overall, our findings proved that acetate promotes skeletal muscle growth and development in young mice via increasing Gm16062 expression.

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Section: Discussionmentioning

confidence: 97%

Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice

Yang,

Zhang,

Liu

et al. 2024

IJMS

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“…In all three time periods, MyOG, and MyHC. MiR-129-5p targets the 3 'UTR region of MEF2A and is repressed in C2C12 cells (45). Mir-129-5p mainly enriches in the cytoskeleton, hydrolase activity, protein binding, and tetrapeptide duplication in GO.…”

Section: Discussionmentioning

confidence: 98%

RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats

Zhao

Xue

et al. 2022

Front. Vet. Sci.

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Muscle differentiation is an essential link in animal growth and development, and microRNA and mRNA are indispensable in skeletal muscle differentiation. To improve the meat quality and production of the Leizhou goat, it is vital to understand the molecular mechanism by which its skeletal muscle differentiates. By RNA sequencing (RNA-SEQ), we established miRNA-mRNA profiles of Leizhou goats at three stages: fetal day 70, 90, and 120. There were 991 differently expressed mRNAs and 39 differentially expressed miRNAs found, with the differentially expressed mRNAs mainly enriched in calcium ion binding, ECM-receptor interaction, and Focal adhesion. CKM and MYH3, two muscle differentiation markers, were significantly differentially expressed during this period. In addition, we found that chi-miR-129-5p, chi-miR-433, and chi-miR-24-3p co-regulate muscle differentiation with their target genes. Finally, we can confirm that muscle differentiation occurred in Leizhou goat between 90 and 120 days of the fetus. This study is helpful to better explore the molecular mechanism of goat muscle differentiation.

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“…Recently, we showed that the expression of miRNAs involved in osteogenic differentiation such as miR-21-5p, miR129-5p and miR-378-5p was increased following a half-marathon. In addition to miR-21-5p, miR129-5p and miR-378-5p are also involved in myogenesis [ 49 , 87 , 88 ].…”

Section: Interconnected Bone and Muscle Molecular Pathwaysmentioning

confidence: 99%

Crosstalk between Bone and Muscles during Physical Activity

Dalle Carbonare,

Minoia,

Zouari

et al. 2023

Cells

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Bone–muscle crosstalk is enabled thanks to the integration of different molecular signals, and it is essential for maintaining the homeostasis of skeletal and muscle tissue. Both the skeletal system and the muscular system perform endocrine activity by producing osteokines and myokines, respectively. These cytokines play a pivotal role in facilitating bone–muscle crosstalk. Moreover, recent studies have highlighted the role of non-coding RNAs in promoting crosstalk between bone and muscle in physiological or pathological conditions. Therefore, positive stimuli or pathologies that target one of the two systems can affect the other system as well, emphasizing the reciprocal influence of bone and muscle. Lifestyle and in particular physical activity influence both the bone and the muscular apparatus by acting on the single system but also by enhancing its crosstalk. Several studies have in fact demonstrated the modulation of circulating molecular factors during physical activity. These molecules are often produced by bone or muscle and are capable of activating signaling pathways involved in bone–muscle crosstalk but also of modulating the response of other cell types. Therefore, in this review we will discuss the effects of physical activity on bone and muscle cells, with particular reference to the biomolecular mechanisms that regulate their cellular interactions.

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MicroRNA-129-5p inhibits C2C12 myogenesis and represses slow fiber gene expression in vitro

Cited by 10 publications

References 43 publications

Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice

Acetate Alleviates Gut Microbiota Depletion-Induced Retardation of Skeletal Muscle Growth and Development in Young Mice

RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats

Crosstalk between Bone and Muscles during Physical Activity

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