MicroRNA‑1301‑3p promotes the progression of non‑small cell lung cancer by targeting Thy‑1 and predicts poor prognosis of patients

Abstract

The role of microRNA (miR)-1301-3p has been investigated in breast cancer and colorectal cancer. Dysregulation of miR-1301-3p expression in non-small cell lung cancer (NSCLC) is speculated to be associated with tumor progression, which was systemically investigated in the present study. Reverse transcription-quantitative PCR analysis was performed to detect miR-1301-3p expression in 124 paired tissue samples and cultured cell lines. The results demonstrated that miR-1301-3p expression was regulated by transfection with miR-1301-3p mimic or inhibitor, and the proliferation, migration and invasion of the transfected cells were assessed via the Cell Counting Kit-8 and Transwell assays. In addition, miR-1301-3p expression was significantly upregulated in NSCLC tissues and cells compared with normal tissues and normal cells, respectively. Notably, upregulated miR-1301-3p expression in NSCLC tissues was significantly associated with the TNM stage, lymph node metastasis and poor prognosis of patients with NSCLC. Furthermore, upregulated miR-1301-3p expression in NSCLC cells promoted cell proliferation, migration and invasion, the effects of which were reversed following miR-1301-3p knockdown. Thy-1 was identified as a direct target of miR-1301-3p, which serves as a tumor promoter in the progression of NSCLC. Taken together, the results of the present study suggest that upregulated miR-1301-3p expression in NSCLC acts as an independent prognostic factor and a tumor promoter by targeting thy-1, thus provides a potential therapeutic target for NSCLC.

Aberrant Expression of miR-1301 in Human Cancer

“…In four lung cancer cell lines, the expression of miR-1301 was higher than that of human normal lung epithelial cells (BEAS-2B) (5,21). Thy-1 is a cell surface glycoprotein, which is closely related to idiopathic pulmonary fibrosis (22) and lung cancer (5). Polymerase I and transcript release factor (PTRF) is also known as cavin-1, and its down-regulation has been shown to promote the progression of prostate cancer, breast cancer, and glioblastoma (30).…”

“…In four lung cancer cell lines, the expression of miR-1301 was higher than that of human normal lung epithelial cells (BEAS-2B) (5,21). Thy-1 is a cell surface glycoprotein, which is closely related to idiopathic pulmonary fibrosis (22) and lung cancer (5).…”

“…miRNA can target different downstream genes, and play an anti-cancer effect or carcinogenic effect in different tumors. Although miR-1301 is down-regulated in 9 tumors, miR-1301 is up-regulated in lung cancer ( 5 , 21 ) and breast cancer ( 3 , 20 ).…”

“…Polymerase I and transcript release factor (PTRF) is also known as cavin-1, and its down-regulation has been shown to promote the progression of prostate cancer, breast cancer, and glioblastoma ( 30 ). In lung cancer, miR-1301 acts as an oncogene by inhibiting the Thy-1 and PTRF genes ( 5 , 21 ).…”

“…miR-1301-3p (miR-1301) is abnormally expressed in 16 cancers. Abnormal expression of miR-1301 is significantly related to the clinicopathological characteristics and prognosis of 4 cancers (4)(5)(6)(7)(8). miR-1301 is related to the anti-tumor effect of epirubicin (EPI) on osteosarcoma and imatinib (IM) on chronic myeloid leukemia (CML).…”