MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway

Abstract

In the present study, the expression of microRNA (miR)‑132 and the mechanism by which it modifies angiogenesis in patients with ischemic cerebrovascular disease (ICD) was investigated. RNA isolation and reverse transcription‑quantitative polymerase chain reaction were used to measure miR‑132 expression in patients with ICD. Inflammatory factors were measured using ELISA kits and western blotting measured B‑cell lymphoma‑2 (Bcl‑2)‑associated X/Bcl‑2 ratio (Bax/Bcl‑2 ratio), nuclear factor (NF)‑κB p65, matrix metalloproteinase‑9 (MMP‑9), vascular cell adhesion molecule‑1 (VCAM‑1) and protein expression of inducible nitric oxide synthase (iNOS), and vascular endothelial growth factor (VEGF) protein expression. miR‑132 expression in patients with ICD was lower compared with healthy volunteers. PC12 cells were used to create an oxygen glucose deprivation (OGD) model. miR‑132 overexpression in an in vitro model was able to reduce tumor necrosis factor‑a, interleukin (IL)‑1β, IL‑6, IL‑8, cyclooxygenase‑2, caspase‑3 and caspase‑9 levels, suppress Bax/Bcl‑2 ratio, NF‑κB p65, MMP‑9, VCAM‑1, iNOS, VEGF protein expression. The results suggested that miR‑132 may modify angiogenesis in patients with ICD by suppressing the NF‑κB pathway and promoting the VEGF pathway, and may develop into a therapy for ICD in future research.

miR‑132 improves the cognitive function of rats with Alzheimer's disease by inhibiting the MAPK1 signal pathway

“…Overall, these results suggested that oxidative stress was decreased and that the antioxidant defense systems were stimulated. Che et al (34) reported that miR-132 overexpression in vitro inhibited iNOS, which is consistent with the present results. Subsequently, inhibition of p38 signaling pathway may improve brain damage, decrease oxidative stress and improve cognitive dysfunction.…”

“…Furthermore, a number of specific miRNAs are dysregulated in patients with AD, including the miRNAs of key genes of AD, such as amyloid precursor protein or beta-secretase 1, or of neuronal functions, such as glutamate receptors (33). Che et al (34) demonstrated that miR-132 can regulate the angiogenesis of patients with cerebral ischemia through NF-κB and vascular endothelial growth factor pathways, and reported that overexpression of miR-132 in vitro could decrease iNOS expression. In the present study, specific binding sites of miR-132 and MAPK1 were found through bioinformatics analysis, and dual-luciferase reporter assay confirmed that miR-132 can negatively target MAPK1 gene.…”

MicroRNA in Acupuncture Studies: Does Small RNA Shed Light on the Biological Mechanism of Acupuncture?

“…Mediating role of miR-9 was suggested as expression level of miR-9 was decreased in middle cerebral artery occlusion rat model; yet, neurological functions and behavioral abnormalities were restored with miR-9 level restoration [51]. Also, increased level of miR-132 influenced on adjusting angiogenesis by suppressing NF- κ B and vascular endothelial growth factor in patients with ischemic cerebrovascular disease [52]. These changes and distinct roles of each miRNAs may possibly provide an intriguing connection between the effect of acupuncture on stroke and these miRNAs.…”

The Progress in Diagnosis and Treatment of Exosomes and MicroRNAs on Epileptic Comorbidity Depression

“…Moreover, exosomes has been considered to be a vector that promotes inter-cell communication and regulates the cell function by delivering proteins, RNA, and other molecular components, with its nature for biocompatibility, stability in the circulation, biological barrier permeability, low immunogenicity, and low toxicity (7,84). And in vitro model experiments have shown that overexpression of miR-132 can reduce the expression of IL-Ib (39,85). In addition, it was reported that IL-1b could induce disruption of the blood-brain barrier (86)(87)(88).…”