MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R

Abstract

Background/Aims: MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression by repressing translation or cleaving RNA transcripts in a sequence-specific manner. Downregulated microRNAs and their roles in cancer development have attracted much attention. A growing body of evidence showed that microRNA-133a (miR-133a) has inhibitory effects on cell proliferation, migration, invasion, and metastasis of osteosarcoma. Methods: MiR-133a expression in human osteosarcoma cell lines and human normal osteoblastic cell line hFOB was investigated by real-time PCR (RT-PCR). The role of miR-133a in human osteosarcoma growth and invasion was assessed in cell lines in vitro and in vivo. Then, luciferase reporter assay validated IGF-1R as a downstream and functional target of miR-133a, and functional studies revealed that the anti-tumor effect of miR-133a was probably due to targeting and repressing of IGF-1R expression. Results: MiR-133a was lower expressed in human osteosarcoma cell lines than human normal osteoblastic cell line hFOB and its effect on inhibiting proliferation, invasion and metastasis is mediated by its direct interaction with the IGF-1R. Furthermore, the tumour-suppressive function of miR-133a probably contributed to inhibiting the activation AKT and ERK signaling pathway. Conclusion: MiR-133a suppresses osteosarcoma progression and metastasis by targeting IGF-1R in human osteosarcoma cells, providing a novel candidate prognostic factor and a potential anti-metastasis therapeutic target in osteosarcoma.

MicroRNA‑495 suppresses cell proliferation and invasion of hepatocellular carcinoma by directly targeting insulin‑like growth factor receptor‑1

“…IGF1R likely performs its functions partly by participating in the regulation of AKT and ERK signalling pathways (26)(27)(28). To determine whether miR-495 is involved in the regulation of AKT and ERK pathways in HCC, we detected the expression levels of AKT, p-AKT, ERK and p-ERK in Hep3B cells transfected with miR-495 mimics or miR-NC.…”

Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression

“…For example, Bi et al (17) suggested that miR-100 serves as a cancer inhibitor in OS development via suppressing OS growth by regulating FGFR3 expression. MicroRNA-133a can prevent the proliferation and invasion of osteosarcoma cells by targeting IGF-1R (18). MiR-542-5p has been found to serve as an oncogene in OS though promoting OS proliferation by targeting HUWE1 (19).…”

Andrographolide Induces Autophagic Cell Death and Inhibits Invasion and Metastasis of Human Osteosarcoma Cells in An Autophagy-Dependent Manner

“…U-2OS and SaOS-2 cells were cultured in McCoy’s 5A (Sigma, M4892) with 10% fetal bovine serum (CLARK, FB25015), MG-63 cells were cultured in MEM (Gibco 41500-034) with 10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 U/mL) in a humidified incubator (Thermo Fisher, Runcorn, Cheshire, UK) with 5% CO 2 at 37°C [22, 23]. …”

“…At 24 hours after transfection, cells were treated with or without AG for additional 24 hours and collected for western blot. Transfected cells were also used for MTT, Immunocytochemistry, Live/dead assay, Wound healing assay, Migration and invasion assays [23]. …”