MicroRNA-140 represses glioma growth and metastasis by directly targeting ADAM9

Liu, Xiaogang; Shan-jun, Wang; Yuan, Aiqin; Yuan, Xunhui; Liu, Bing

doi:10.3892/or.2016.5007

Cited by 28 publications

(20 citation statements)

References 45 publications

(52 reference statements)

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“…Previous studies have demonstrated that miRNAs negatively regulate target gene expression by binding to the 3'UTR of target genes (26)(27)(28). In the present study, MTDH was identified as a novel target gene of miR-154 in GC.…”

Section: Discussionsupporting

confidence: 51%

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

Qiao¹,

Cao²,

Yang³

2017

Oncology Letters

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Abstract. MicroRNAs (miRNAs) are a group of non-protein-coding, highly conserved single-stranded RNA molecules. The abnormal expression of miRNAs has been demonstrated to have an important function in the carcinogenesis and progression of gastric cancer. microRNA-154 (miR-154) has been reported to be downregulated in non-small cell lung, colorectal and prostate cancer. However, the expression and roles of miR-154 in gastric cancer remain to be established. The present study measured the expression levels of miR-154 in gastric cancer tissues and cell lines. miR-154 was found to be significantly downregulated in gastric cancer tissues and cell lines. In addition, functional studies indicated that the overexpression of miR-154 inhibited the proliferation, migration and invasion of gastric cancer cells. Using TargetScan, a dual luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction and western blot analysis, metadherin (MTDH) was revealed as a novel miR-154 target. In addition, knocking down MTDH lead to a similar effect as overexpressing-154 in gastric cells. The present findings indicate that miR-154 was downregulated in gastric cancer, and inhibited tumor behaviors of gastric cancer cells partially through the downregulation of MTDH. Therefore, the miR-154/MTDH axis may be a novel therapeutic to treat patients with gastric cancer.

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Section: Discussionsupporting

confidence: 51%

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

Qiao¹,

Cao²,

Yang³

2017

Oncology Letters

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“…Furthermore, multivariate analysis indicated ADAM9 expression as an independent marker of poor survival (52). Functional assays revealed that ADAM9 plays important roles in glioma growth and metastasis (19,53). These studies all indicated that ADAM9 may be a novel and promising target for therapeutic intervention in patients with glioma.…”

Section: Discussionmentioning

confidence: 90%

“…miRNAs play important roles in diverse biological processes, such as cell proliferation, cycle, death, differentiation, metastasis and drug resistance (16)(17)(18). The deregulation of miRNAs has been detected in a wide variety of human cancers, such as glioma (19), prostate cancer (20), gastric cancer (21), breast cancer (22) and lung cancer (23). Increasing evidence suggested that the abnormal expression of some specific miRNAs are closely related to tumorigenesis and tumor development, and some miRNAs also act as tumor suppressors or oncogenes depending on the characteristic of their target genes (24-26).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-543 inhibits proliferation, invasion and induces apoptosis of glioblastoma cells by directly targeting ADAM9

Zhang

2017

Molecular Medicine Reports

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Glioma is the most common type of malignant brain tumor in humans and accounts for 81% of all malignant brain tumor cases in adults. The abnormal expression of microRNAs (miRs) has been reported to be important in the formation and progression of various types of human cancer, including glioblastoma (GBM). Therefore, studies into the expression, and roles of microRNAs as diagnostic and prognostic markers, as well as their therapeutic value for patients with GBM are warranted. The expression and roles of miR‑543 have been reported in several types of human cancer. However, the role of miR‑543 in GBM remains unclear. In the current study, the expression pattern of miR‑543 in GBM, the effects of miR‑543 on GBM cells and the underlying molecular mechanism was determined. The results of the present study demonstrated that miR‑543 was significantly downregulated in GBM tissue samples and cell lines. Furthermore, the upregulation of miR‑543 inhibited GBM cell proliferation and invasion, as well as promoted cell apoptosis. In addition, a disintegrin and metalloproteinase 9 (ADAM9) was identified to be a direct target gene of miR‑543. Furthermore, ADAM9 was significantly upregulated in GBM tissue samples and its expression was inversely correlated with miR‑543 expression in GBM tissue, suggesting that miR‑543 downregulation may contribute to ADAM9 upregulation in GBM. Finally, the results of the rescue experiment indicated that ADAM9 overexpression significantly reversed the effects of miR‑543 on the proliferation, invasion and apoptosis of GBM cells, suggesting that miR‑543 serves as a tumor suppressor in GBM through ADAM9 regulation. Overall, these findings indicate that the miR‑543/ADAM9 signaling pathway may provide as a potential therapeutic strategy for GBM.

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“…To date, the molecular mechanisms underlying the occurrence and development of gliomas remain unclear. Gliomas can be associated with several risk factors, including tumour origin, genetic factors, biochemical environment, ionizing radiation, nitroso compounds, air pollution, unhealthy lifestyle habits and infection (3,4). At present, surgery combined with radiotherapy, chemotherapy and targeted therapy is the standard treatment for patients with glioma (5).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-103 suppresses glioma cell proliferation and invasion by targeting the brain-derived neurotrophic factor

Liu

Song

2017

Mol Med Report

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Glioma is the most common and aggressive of malignant brain tumours. MicroRNAs (miRNAs/miRs) are involved in tumour development of various human cancers, including glioma. Therefore, miRNAs may have potential tumour diagnostic, prognostic and therapeutic values in human glioma. miR‑103 is abnormally expressed in various human cancer types. However, the detailed expression pattern, biological functions and underlying molecular mechanism of miR‑103 in glioma remain unclear. Therefore, the present study aimed to investigate the expression, biological roles and underlying mechanisms of miR‑103 in glioma. Results of the present study demonstrated that miR‑103 was significantly down‑regulated in glioma tissues and cell lines. Functional experiments demonstrated that miR‑103 overexpression inhibited the proliferation and invasion of glioma cells in vitro. Additionally, brain‑derived neurotrophic factor (BDNF) was identified as a direct functional target of miR‑103 in glioma. Furthermore, mRNA and protein expression levels of BDNF were highly upregulated in glioma tissues compared with normal brain tissues. Spearman's correlation analysis indicated a negative association between miR‑103 and BDNF mRNA expression levels in glioma tissues. Furthermore, rescue experiments demonstrated that BDNF up‑regulation reversed the suppressive effects of miR‑103 on glioma cell proliferation and invasion. Therefore, the authors of the present study hypothesized that the interaction between miR‑103 and BDNF serves a role in glioma progression and, in the future, may serve as a therapeutic target for glioma treatment.

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MicroRNA-140 represses glioma growth and metastasis by directly targeting ADAM9

Cited by 28 publications

References 45 publications

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

MicroRNA-543 inhibits proliferation, invasion and induces apoptosis of glioblastoma cells by directly targeting ADAM9

MicroRNA-103 suppresses glioma cell proliferation and invasion by targeting the brain-derived neurotrophic factor

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