MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma

Zhao, Zhiguo; Gao, Dan; T, Ma; Zhang, Liping

doi:10.18632/aging.101791

Cited by 21 publications

(14 citation statements)

References 16 publications

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“…Recently, a number of miRNAs have been reported to be dysregulated in various cancer types, including HNSCC 12,13. For instance, miR-141 shows decreased expression in HNSCC tissues and exerts tumor-suppressive roles by inhibiting the expression of epidermal growth factor receptor (EGFR) 14. miR-200 facilitates the epithelial-mesenchymal transition (EMT) in HNSCC cells and promotes HNSCC progression 15.…”

Section: Introductionmentioning

confidence: 99%

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

Chen¹,

Zhang²,

Sun³

et al. 2019

OTT

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BackgroundHead and neck squamous cell carcinoma (HNSCC) is among the most common malignant tumors worldwide. This study, investigated the role of microRNA (miR)-762 in regulating HNSCC progression.Materials and methodsThe expression levels of miR-762 in HNSCC tissues were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Statistical analyses were performed to investigate the association of miR-762 with clinicopathological features in patients with HNSCC. Cell proliferation and migration were examined by cell counting (CCK-8) and IncuCyte assays. Target genes of miR-762 were screened using bioinformatics tools and microarrays, and confirmed using a luciferase activity reporter assay, qRT-PCR and Western blot analysis. Recuse experiments were performed to detect whether target genes mediated the effects of miR-762 on HNSCC cells. The in vivo effects of miR-762 were verified using tumor xenografts.ResultsHNSCC clinical specimens showed high expression levels of miR-762, which positively correlated with tumor-node-metastasis (TNM) stage and poor prognosis of HNSCC. miR-762 overexpression promoted the proliferation and migration of HNSCC cells in vitro. In addition, overexpression of miR-762 upregulated the expression of phosphorylated AKT (p-AKT) and mesenchymal markers (N-cadherin and vimentin), but suppressed epithelial marker (E-cadherin) expression. miR-762 also promoted HNSCC tumor growth in vivo. PH domain and leucine-rich repeat protein phosphatase 2 (PHLPP2) and Forkhead box O4 (FOXO4) were direct target genes of miR-762. HNSCC tissues had low expression levels of PHLPP2 and FOXO4, showing a negative correlation with miR-762 expression. Moreover, silencing of PHLPP2 and FOXO4 mimicked the tumor-promotive effects of miR-762 on HNSCC cells. Notably, overexpression of PHLPP2 and FOXO4 abolished the pro-tumoral function of miR-762 on cell proliferation and migration.ConclusionmiR-762 promotes HNSCC progression by targeting PHLPP2 and FOXO4. Therefore, miR-762 might be a potential diagnostic or therapeutic target for HNSCC.

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Section: Introductionmentioning

confidence: 99%

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

Chen¹,

Zhang²,

Sun³

et al. 2019

OTT

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“…Risk factors for OSCC include alcohol consumption, tobacco smoking and adverse irritation of the oral mucosa ( 2 , 3 ). Although great progress has been made in the treatment of OSCC, the 5-year survival rate has not improved much over the past two decades ( 4 , 5 ). Therefore, further understanding of the pathogenesis of OSCC may help to predict cancer progression and provide novel molecular targets for cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4

Zhang

Sun

2021

Int J Oncol

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MicroRNAs (miRNAs/miR) often contribute to the progression of oral squamous cell carcinoma (OSCC) via the regulation of mRNA. The present study aimed to investigate the role of miR-198 in OSCC pathogenesis and explore the underlying mechanism. Reverse transcription-quantitative (RT-q)PCR was performed to determine miR-198 expression in OSCC tissues and cell lines, and univariate and multivariate analyses were applied to evaluate the survival of patients with OSCC. The effects of miR-198 on OSCC cell lines were studied in vitro and in vivo. A set of epithelial-mesenchymal transition (EMT) markers were detected to determine whether miR-198 was involved in EMT. Lastly, using luciferase assays, a novel target of miR-198 was identified and the effect of the new target gene of miR-198 on cell proliferation and invasion was also studied. It was identified that miR-198 expression was decreased in OSCC tissues and cell lines, and low expression of miR-198 was associated with poor overall survival and disease-free survival. Overexpression of miR-198 appeared to significantly inhibit the proliferation, invasion and EMT of OSCC cells. Moreover, the luciferase assay results showed that miR-198 interacted with cyclin-dependent kinase 4 (CDK4) by directly targeting the miRNA-binding site in the CDK4 sequence, and RT-qPCR results showed that CDK4 expression was increased in OSCC tissues and cell lines. In addition, transfection of small interfering RNA against CDK4 in OSCC cells showed similar inhibitory effects on cell proliferation, invasion and EMT, whereas CDK4 overexpression in OSCC cells partially reversed the inhibitory effects of the miR-198 mimic. The present results indicated that miR-198 suppressed OSCC tumour growth and metastasis by directly targeting CDK4 expression. Thus, miR-198 may be a potential therapeutic target in the treatment of OSCC.

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“…MicroRNAs are a type of short (approximately 20~25 nucleotide), single-stranded non-coding RNAs, which are generally expressed in a diversity of tissues and cell types and mediate post-transcriptional gene silencing via binding to mRNA 3'UTR (35)(36)(37). Accumulating studies on the biological behaviors of miRNAs in the development, prognosis, proliferation, apoptosis, and differentiation have attracted the people's attention (38).…”

Section: Introductionmentioning

confidence: 99%

Melatonin Inhibits the Progression of Oral Squamous Cell Carcinoma via Inducing miR-25-5p Expression by Directly Targeting NEDD9

Wang¹,

Tao

Mao

et al. 2020

Front. Oncol.

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Melatonin exerts anti-cancer roles in various types of cancers. However, to the best of our knowledge, its role in oral squamous cell carcinoma (OSCC) is unknown. The present study aimed to investigate the role of melatonin and its underlying mechanism in OSCC. MTT, colony formation, wound healing, and transwell invasion assays proved that melatonin played anti-tumor effects in OSCC cells by inhibiting cell viability, proliferation, migration, and invasion in a concentration-dependent manner. The RT-qPCR analysis showed that miR-25-5p was significantly upregulated after melatonin treatment. Further, miR-25-5p might be involved in melatonin-induced inhibitory effects on the biological behavior of OSCC. The expression of miR-25-5p was decreased in tumor tissues and OSCC cells detected by RT-qPCR. MTT assay, colony formation assay, and TUNEL staining indicated miR-25-5p overexpression inhibited OSCC cell viability, proliferation, and induced OSCC cell apoptosis. Furthermore, wound healing, transwell invasion assay, and animal experiments suggested that miR-25-5p might exert suppressive effects on the migration, invasion, and tumor formation of OSCC cells, while miR-25-5p knockdown exhibited the opposite effects in OSCC cells. Bioinformatics analysis, western blot analysis, and luciferase reporter assay suggested that neural precursor cell expressed developmentally downregulated protein 9 (NEDD9) was proved to be a putative target for miR-25-5p. The role of NEDD9 in inhibiting OSCC cell proliferation, invasion, and migration was verified with NEDD9 siRNA transfection. Thus, melatonin exerted anti-proliferative, anti-invasive, and anti-migrative effects on OSCC via miR-25-5p/NEDD9 pathway. Melatonin could be applied as a potential novel drug on treating OSCC.

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MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma

Cited by 21 publications

References 16 publications

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

<p>miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4</p>

MicroRNA‑198 suppresses tumour growth and metastasis in oral squamous cell carcinoma by targeting CDK4

Melatonin Inhibits the Progression of Oral Squamous Cell Carcinoma via Inducing miR-25-5p Expression by Directly Targeting NEDD9

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