MicroRNA-143 as a potential tumor suppressor in cancer: An insight into molecular targets and signaling pathways

Asghariazar, Vahid; Kadkhodayi, Mahtab; Sarailoo, Mehdi; Jolfayi, Amir Ghaffari; Baradaran, Behzad

doi:10.1016/j.prp.2023.154792

Cited by 7 publications

(2 citation statements)

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“…MicroRNAs’ expression profiles seem to differ depending on the tissue studied and the cancer differentiation state, making the classification of each biomolecule difficult. MicroRNA-143 has been reported to be up- or downregulated in the tumor microenvironment of different tissues [ 47 ]. However, when it comes to cervical cancer, many studies agree on miR-143’s down-regulation throughout all the stages of cancer progression when compared to healthy tissue [ 23 , 24 , 27 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNA-143 has been reported to be up- or downregulated in the tumor microenvironment of different tissues [ 47 ]. However, when it comes to cervical cancer, many studies agree on miR-143’s down-regulation throughout all the stages of cancer progression when compared to healthy tissue [ 23 , 24 , 27 , 47 ]. Our study’s findings are consistent with this data, given that miR-143 levels are decreased in SiHa and CaSki cell lines in comparison with the HCK1T normal cervical keratinocytes.…”

Section: Discussionmentioning

confidence: 99%

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HPV16 E6 Oncogene Contributes to Cancer Immune Evasion by Regulating PD-L1 Expression through a miR-143/HIF-1a Pathway

Konstantopoulos,

Leventakou,

Saltiel

et al. 2024

Viruses

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Human Papillomaviruses have been associated with the occurrence of cervical cancer, the fourth most common cancer that affects women globally, while 70% of cases are caused by infection with the high-risk types HPV16 and HPV18. The integration of these viruses’ oncogenes E6 and E7 into the host’s genome affects a multitude of cellular functions and alters the expression of molecules. The aim of this study was to investigate how these oncogenes contribute to the expression of immune system control molecules, using cell lines with integrated HPV16 genome, before and after knocking out E6 viral gene using the CRISPR/Cas9 system, delivered with a lentiviral vector. The molecules studied are the T-cell inactivating protein PD-L1, its transcription factor HIF-1a and the latter’s negative regulator, miR-143. According to our results, in the E6 knock out (E6KO) cell lines an increased expression of miR-143 was recorded, while a decrease in the expression of HIF-1a and PD-L1 was exhibited. These findings indicate that E6 protein probably plays a significant role in enabling cervical cancer cells to evade the immune system, while we propose a molecular pathway in cervical cancer, where PD-L1′s expression is regulated by E6 protein through a miR-143/HIF-1a axis.

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