2020
DOI: 10.1080/21655979.2020.1729928
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MicroRNA-145 overexpression inhibits neuroblastoma tumorigenesis in vitro and in vivo

Abstract: Neuroblastoma (NB) is responsible for 15% of all childhood cancer deaths. Despite advances in treatment and disease management, the overall 5-year survival rates remain poor in high-risk disease (25-40%). It is well known that miR-145 functions as a tumor suppressor in several types of cancer. However, the impact of miR-145 on NB is still ambiguous. Our aim was to investigate the potential tumor suppressive role and mechanisms of miR-145 in high-risk neuroblastoma. Expression levels of miR-145 in tissues and c… Show more

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Cited by 29 publications
(23 citation statements)
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“…Acridine Orange assays show that high levels of miR-145 increase apoptosis of zebrafish embryonic cells, suggesting that NCCs may die before differentiation ( Figure 6 ). This finding agrees with data gathered in embryonic and cancer cultured cell lines, wherein miR-145 overexpression promotes apoptosis and reduces cell viability [ 16 , 34 , 69 , 77 , 78 ]. These observations as a whole could suggest that miR-145 participates in the control of cell death regulation in both normal and pathological conditions.…”
Section: Discussionsupporting
confidence: 92%
“…Acridine Orange assays show that high levels of miR-145 increase apoptosis of zebrafish embryonic cells, suggesting that NCCs may die before differentiation ( Figure 6 ). This finding agrees with data gathered in embryonic and cancer cultured cell lines, wherein miR-145 overexpression promotes apoptosis and reduces cell viability [ 16 , 34 , 69 , 77 , 78 ]. These observations as a whole could suggest that miR-145 participates in the control of cell death regulation in both normal and pathological conditions.…”
Section: Discussionsupporting
confidence: 92%
“…Different types of cancer have different miRNA expression profiles, which can distinguish adjacent normal tissues [19][20][21]. The discovery of miRNA not only provides new insights into the regulation mechanism of cell proliferation, differentiation, apoptosis but also promotes the development of diagnosis and treatment of diseases [22,23]. The biological characteristics of PC are closely related to the up-regulation and down-regulation of miRNA expression in PC [11,24].…”
Section: Discussionmentioning
confidence: 99%
“…Post-synthetic labeling of RNA is applied for preparing fluorescently modified RNAs to alleviate the synthetic bottleneck due to steric inhibition from bulky fluorophores to RNAs [6][7][8]. And coupling reaction of N-hydroxysuccinimide ester (NHS ester) with primary amino group is a useful strategy for post-synthetic modification of RNAs [5,9,10]. Natural RNAs lack reactive primary amino groups, and site-specific introduction of primary amino to RNAs can trigger the conjugation between NHS ester and the specific site [7].…”
Section: Introductionmentioning
confidence: 99%
“…The aminoally-modified rbA71 (aa-rbA71) was synthesized by incorporation of 5-aminoally-UTP into Site 22 of rbA71 (Site 22 is labeled in red in Figure 1(a)) to obtain aa-rbA71 via PLOR (Position-specific Labeling of RNA) (the detailed synthesis is listed in Material and Methods), which is a novel platform for sitespecific labeling of RNA [15,21]. aa-rbA71 reacted with NHS ester-linked Tide Fluor 3 (NHS-TF3) to generate fluorescent-labeled rbA71 following the reported condition [7,[10][11][12][13][14][15][16][17][22][23][24][25][26]. Various conditions for NHS coupling reaction were reported.…”
Section: Introductionmentioning
confidence: 99%
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