MicroRNA‐145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB

Yan, Guijun; Zhang, Lianxiao; Fang, Ting; Zhang, Qun; Wu, Shaogen; Jiang, Yue; Sun, Haixiang; Hu, Yali

doi:10.1016/j.febslet.2012.06.048

Cited by 79 publications

(61 citation statements)

References 43 publications

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“…), and miR-99a (EDF1, IGFBP2, etc.) were predicted in this study, and these have already been validated and reported as ovarian function-related genes (Hossain et al, 2009;Hossain et al, 2012b;Yan et al, 2012). One previous study has demonstrated that miR-125b, miR-145, and miR-99a were expressed at significantly higher levels in the ovaries of pregnant goats compared to non-pregnant goats (Zhang et al, 2013).…”

Section: Discussionsupporting

confidence: 75%

“…Similarly, another study reported that treatment of cultured bovine granulosa cells with forskolin (an adenylate cyclase agonist) to promote luteinisation was followed by a decrease in the levels of miR-125b and miR-145 (McBride et al, 2012). Additionally, Yan et al (2012) reported attenuation of activin induced proliferation of mouse granulosa cells by miR-145 targeting ActRIB. Therefore, miR-125b, miR-145, and miR-99a may play a specific role in the regulation of ovarian function and mammalian reproduction.…”

Section: Discussionmentioning

confidence: 89%

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Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Shen¹,

Chen²,

Jia³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The role of microRNAs (miRNAs) in the regulation of mammalian reproduction has been demonstrated previously. However, only a few studies have assessed the role of miRNAs in the reproduction processes of sheep. The elucidation of miRNA expression profiles in the ovaries of different sheep breeds representing fecundity extremes will be useful in understanding the roles of miRNAs in sheep reproduction. In this study, two small RNA libraries were constructed from ovary tissue taken from Qira black sheep and Hetian sheep during the estrous period and then sequenced using the Solexa sequencing method. We obtained 9,565,212 and 9,563,426 high-quality reads from Qira black sheep and Hetian sheep, respectively. In total, 531 miRNAs, including 98 putative miRNAs, were identified. Among the conserved miRNAs, 125 known miRNAs were significantly differentially expressed in the Qira black sheep and Hetian sheep libraries, with 24 upregulated and 101 downregulated in the Hetian sheep compared to the Qira black sheep. Four differentially expressed miRNAs were analyzed using real-time 7357 miRNA expression analysis in sheep ovaries ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7356-7367 (2015) quantitative PCR to validate the reliability of the Solexa sequencing results. These results provide a foundation for future research on the regulation of miRNAs in sheep fertility and enrich the sheep miRNA databases.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 89%

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Shen¹,

Chen²,

Jia³

et al. 2015

Genet. Mol. Res.

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“…The expression of the second activin type II receptor, ActRIIB, is controlled by five miRNAs in the context of normal kidney homeostasis, whereas kidney neoplasia in young children is characterized by down-regulation of miR-141, miR-192, miR-194, miR-200c, and miR-215, and consequent up-regulation of ActRIIB in the developing nephroblastomas (Senanayake et al 2012). The expression of activin/nodal type I receptors ActRIB/ALK-4 and ActRIC/ALK-7 is also negatively regulated by miRNAs; in normal ovarian granulosa cells, ALK-4 mRNA is targeted by miR-145, which controls cell proliferation (Yan et al 2012). During erythropoiesis, the ALK-4 mRNA is targeted by miR-24; ALK-4 down-regulation is required so that early stages of erythroid cell differentiation can progress .…”

Section: Control Of Tgf-b Receptor Expression By Micrornasmentioning

confidence: 99%

Signaling Receptors for TGF-β Family Members

Heldin

Moustakas

2016

Cold Spring Harb Perspect Biol

575

498

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Transforming growth factor b (TGF-b) family members signal via heterotetrameric complexes of type I and type II dual specificity kinase receptors. The activation and stability of the receptors are controlled by posttranslational modifications, such as phosphorylation, ubiquitylation, sumoylation, and neddylation, as well as by interaction with other proteins at the cell surface and in the cytoplasm. Activation of TGF-b receptors induces signaling via formation of Smad complexes that are translocated to the nucleus where they act as transcription factors, as well as via non-Smad pathways, including the Erk1/2, JNK and p38 MAP kinase pathways, and the Src tyrosine kinase, phosphatidylinositol 3 0 -kinase, and Rho GTPases.

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“…Several studies have already reported effects of specific miRNAs on different aspects of granulosa cell function , including steroidogenesis (Yao et al 2010, Xu et al 2011, Yin et al 2012, Dai et al 2013, proliferation (Yao et al 2010, Yan et al 2012, Dai et al 2013, survival (Carletti et al 2010, Yang et al 2012, terminal differentiation (Kitahara et al 2013), and cumulus expansion (Yao et al 2014). With a few exceptions (Carletti et al 2010, Kitahara et al 2013, most evidence on follicular roles of miRNAs has been obtained using cultured cells, particularly rodent cells, and in some cases actual changes in the expression of these miRNAs during follicle development have not been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Characterization of microRNAs differentially expressed during bovine follicle development

Sontakke¹,

Mohammed²,

McNeilly³

et al. 2014

Reproduction

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Several different miRNAs have been proposed to regulate ovarian follicle function; however, very limited information exists on the spatiotemporal patterns of miRNA expression during follicle development. The objective of this study was to identify, using microarray, miRNA profiles associated with growth and regression of dominant-size follicles in the bovine monovular ovary and to characterize their spatiotemporal distribution during development. The follicles were collected from abattoir ovaries and classified as small (4-8 mm) or large (12-17 mm); the latter were further classified as healthy or atretic based on estradiol and CYP19A1 levels. Six pools of small follicles and individual large healthy (nZ6) and large atretic (nZ5) follicles were analyzed using Exiqon's miRCURY LNA microRNA Array 6th gen, followed by qPCR validation. A total of 17 and 57 sequences were differentially expressed (greater than or equal to twofold; P!0.05) between large healthy and each of small and large atretic follicles respectively. Bovine miRNAs confirmed to be upregulated in large healthy follicles relative to small follicles (bta-miR-144, bta-miR-202, bta-miR-451, bta-miR-652, and bta-miR-873) were further characterized. Three of these miRNAs (bta-miR-144, bta-miR-202, and bta-miR-873) were also downregulated in large atretic follicles relative to large healthy follicles. Within the follicle, these miRNAs were predominantly expressed in mural granulosa cells. Further, body-wide screening revealed that bta-miR-202, but not other miRNAs, was expressed exclusively in the gonads. Finally, a total of 1359 predicted targets of the five miRNAs enriched in large healthy follicles were identified, which mapped to signaling pathways involved in follicular cell proliferation, steroidogenesis, prevention of premature luteinization, and oocyte maturation.

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MicroRNA‐145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB

Cited by 79 publications

References 43 publications

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Signaling Receptors for TGF-β Family Members

Characterization of microRNAs differentially expressed during bovine follicle development

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