microRNA‐146a downregulates IL‐17 and IL‐35 and inhibits proliferation of human periodontal ligament stem cells

Zhao, Sen; Cheng, Yawei; Kim, Jong Ghee

doi:10.1002/jcb.28659

Cited by 16 publications

(10 citation statements)

References 16 publications

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“…Among all 43 included articles, a total of 563 miRNAs were identified reporting fold changes in miRNA expression between health and periodontal/peri-implant disease (Tables S9 and S10). Due to extensiveness and limitations in the reported data, only 16 miRNAs were selected to perform a meta-analysis and were chosen if fold changes values of the same miRNA were reported in both animal and human studies or individually, in more than two and four articles from animal and human studies, respectively [13,15,17,18,[20][21][22][23]26,28,29,[31][32][33]35,37,38,41,42,[44][45][46][47][48][49][50][51].…”

Section: Meta-analysis For Mirna Expressionmentioning

confidence: 99%

Expression of MicroRNAs in Periodontal and Peri-Implant Diseases: A Systematic Review and Meta-Analysis

Asa’ad

Garaicoa-Pazmiño

Dahlin

et al. 2020

IJMS

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Aim: The purpose of this review was to evaluate the expression patterns of miRNAs in periodontal and peri-implant diseases, while identifying potential miRNAs with the greatest diagnostic ability as an oral fluid biomarker. Materials and methods: Human and animal studies were included when evaluating expression of miRNAs between health and different forms/stages of diseases, in which microarray and/or real-time polymerase chain reaction (RT-PCR) was carried out to detect fold changes in gene expression. After full-text analysis, 43 articles were considered for a qualitative assessment, and 16 miRNAs were selected to perform meta-analysis. Results: Based on human studies, results showed an overall upregulation of most of the evaluated miRNAs in periodontitis, with miRNA-142-3p and miRNA-146a being the most conclusive on both microarray and RT-PCR values and potentially serving as diagnostic biomarkers for disease activity. Conversely, miR-155 was the only miRNA revealing a statistically significant difference (SSD) (p < 0.05*) in experimental periodontitis models from RT-PCR values. Scarce scientific evidence is available from peri-implant diseases, however, most explored miRNAs in peri-implantitis were downregulated except for miR-145. Conclusions: Although our results revealed that a distinct differential expression of specific miRNAs can be noted between the state of health and disease, future research remains necessary to explore the functional role of specific miRNAs and their potential as therapeutic targets in periodontal and peri-implant diseases. MeSH Terms: periodontitis, peri-implantitis, epigenomics, microarray analysis, real-time polymerase chain reaction, microRNAs. Clinical relevance: Scientific background: Although most research identified different expression levels of miRNAs in periodontal and peri-implant diseases compared to their counterparts, their actual role in the pathogenesis of these conditions remains unclear. Therefore, we aimed to present a systematic review and meta-analysis on the expression patterns of miRNAs in periodontitis and peri-implantitis, while identifying potential miRNAs with the greatest diagnostic ability as an oral fluid biomarker. Principal findings: In periodontitis-related studies, miRNA-142-3p and miRNA-146a were the most conclusive on both microarray and RT-PCR values. Scarce scientific evidence is available from peri-implant diseases. Practical implications: Both miRNA-142-3p and miRNA-146a might serve as future diagnostic biomarkers for disease activity in periodontitis. Yet, future research remains necessary to explore the functional role of specific miRNAs and their potential as therapeutic targets in periodontal and peri-implant diseases.

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Section: Meta-analysis For Mirna Expressionmentioning

confidence: 99%

Expression of MicroRNAs in Periodontal and Peri-Implant Diseases: A Systematic Review and Meta-Analysis

Asa’ad

Garaicoa-Pazmiño

Dahlin

et al. 2020

IJMS

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“…Periodontitis is a ubiquitous periodontal tissue immune inflammatory disease caused by bacteria (24). The regenerative ability of hPDLSCs is readily affected by the presence of inflammatory mediators such as TNF-α, IL-6, IL-11 and IL-17 in the periodontal environment (25,26). Among these, TNF-α is an important member of the TNF family, showing its effect primarily through the NF-κB or MAPK signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Effect of TNF‑α on the proliferation and osteogenesis of human periodontal mesenchymal stem cells

Wang¹,

Li²,

Jiang³

et al. 2021

Exp Ther Med

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The aim of the present study was to investigate the effect of tumor necrosis factor-α (TNF-α) on the proliferation and osteogenesis of human periodontal mesenchymal stem cells (hPDLSCs). Antigen expression in hPDLSCs was detected by flow cytometry. hPDLSCs were divided into four groups: A control group with no TNF-α treatment, and three experimental groups treated with 0.1, 1 and 10 ng/ml TNF-α, respectively. The effect of TNF-α on proliferation of hPDLSCs in vitro was detected using a Cell Counting Kit-8 assay. Differentiation into an osteogenic lineage was detected by alkaline phosphatase sand alizarin red staining, and the mRNA and protein expression levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and type I collagen (Col-I) were detected using reverse transcription-quantitative PCR and western blot respectively. Following treatment with 10 ng/ml TNF-α, proliferation was significantly increased compared with an untreated control group (P<0.01). Additionally, there was a significant inhibition of alkaline phosphatase enzyme activity, alizarin red mineralization node size, and in the gene and protein expression levels of osteogenic differentiation markers, including Runx2, OCN and COL-I (all, P<0.05). Taken together, the results indicated that treatment with 10 ng/ml TNF-α promoted the proliferation of hPDLSCs in vitro and inhibited osteogenic differentiation of hPDLSCs, providing an experimental basis for regulation of hPDLSC-mediated periodontal tissue regeneration.

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“…Thus, through causing the imbalance between Th17 cells and Tregs, resident PDLSCs in the periodontitis niche lead to increased release of proinflammatory factors and indirectly exacerbated immune disorders 26,41 . Interestingly, decreased expression of miR‐146a in P‐PDLSCs promotes their proliferation with elevated expression of IL‐35, an anti‐inflammatory cytokine. 83 IL‐35 has been reported to effectively alleviate periodontal bone loss in periodontitis models by regulating the Th17/Tregs balance in local periodontal tissues.…”

Section: Impaired Pdlscs Facilitate the Periodontitis Nichementioning

confidence: 99%

Periodontal ligament stem cells in the periodontitis niche: inseparable interactions and mechanisms

Zhang

Deng

Tang

2021

Journal of Leukocyte Biology

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Periodontitis is characterized by the periodontium's pathologic destruction due to the host's overwhelmed inflammation to the dental plaque. The bacterial infections and subsequent host immune responses have shaped a distinct microenvironment, which generally affects resident periodontal ligament stem cells (PDLSCs). Interestingly, recent studies have revealed that impaired PDLSCs may also contribute to the disturbance of periodontal homeostasis. The putative vicious circle underlying the interesting “positive feedback” of PDLSCs in the periodontitis niche remains a hot research topic, whereas the inseparable interactions between resident PDLSCs and the periodontitis niche are still not fully understood. This review provides a microscopic view on the periodontitis progression, especially the quick but delicate immune responses to oral dysbacterial infections. We also summarize the interesting crosstalk of the resident PDLSCs with their surrounding periodontitis niche and potential mechanisms. Particularly, the microenvironment reduces the osteogenic properties of resident PDLSCs, which are closely related to their reparative activity. Reciprocally, these impaired PDLSCs may disrupt the microenvironment by aggravating the host immune responses, promoting aberrant angiogenesis, and facilitating the osteoclastic activity. We further recommend that more in‐depth studies are required to elucidate the interactions of PDLSCs with the periodontal microenvironment and provide novel interventions for periodontitis.

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microRNA‐146a downregulates IL‐17 and IL‐35 and inhibits proliferation of human periodontal ligament stem cells

Cited by 16 publications

References 16 publications

Expression of MicroRNAs in Periodontal and Peri-Implant Diseases: A Systematic Review and Meta-Analysis

Expression of MicroRNAs in Periodontal and Peri-Implant Diseases: A Systematic Review and Meta-Analysis

Effect of TNF‑α on the proliferation and osteogenesis of human periodontal mesenchymal stem cells

Periodontal ligament stem cells in the periodontitis niche: inseparable interactions and mechanisms

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