MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer

Liu, Qin; Wang, Wei; Yang, Xiongfa; Zhao, Dong-Xiao; Li, Fangqiong; Wang, Hai

doi:10.3892/or.2016.4788

Cited by 35 publications

(24 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Accumulating evidence suggested that miR-146a acted as a tumor suppressor or an oncogene in multiple cancers. For example, miR-146a had a low expression in HCC tissues ( 24 ), breast cancer ( 25 ). On the contrary, miR-146a was found to be elevated in colorectal cancer ( 26 ), oral carcinoma ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4

Shang

Shao

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

Previous studies have explored the functions of microRNA (miR)-146a in different types of cancer through mediating different targets. However, the roles of miR-146a in malignant melanoma (MM) cell migration and invasion remain largely elusive. In the present study, the potential molecular function of miR-146a in MM was investigated. Reverse transcription-quantitative polymerase chain reaction was utilized to detect miR-146a expression in MM tissues and cell lines. A Transwell assay was performed to confirm the ability of migration and invasion. A luciferase assay and biological analysis were used to predict and determine the targets of miR-146a. The expression of miR-146a was upregulated in melanoma tissues and cell lines. Clinicopathological analysis indicated that the miR-146a level was negatively correlated with the progression of melanoma. Abnormal expression of miR-146a promoted cell migration and invasion in MM cells. Additionally, it was also observed that Mothers against decapentaplegic homolog 4 (SMAD4) was a novel target of miR-146a in MM. SMAD4 was negatively associated with miR-146a in MM and abnormal expression of SMAD4 attenuated miR-146a-mediated promotion of cell migration and invasion. In conclusion, miR-146a functioned as an oncogene by directly targeting SMAD4 and it may be a novel diagnostic and therapeutic marker of MM.

show abstract

Section: Discussionmentioning

confidence: 99%

miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4

Shang

Shao

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…In addition, miR‐146a suppressed breast cancer metastasis to the lung in a mouse model (Hurst et al, ). Another group also found miR‐146a to act as a tumor suppressor by validating its targeting of RhoA in MDA‐MB‐231 cells, inhibiting migration (Liu, Wang, et al, ). IRAK1 and TRAF6 were further validated as targets, and miR‐146a was also implicated in enhancing apoptosis (Liu, Liu, et al, ).…”

Section: Role Of Mir‐146a In Specific Cancersmentioning

confidence: 97%

miR‐146a‐5p: Expression, regulation, and functions in cancer

2019

View full text Add to dashboard Cite

Cancer as we know it is actually an umbrella term for over 100 very unique malignancies in various tissues throughout the human body. Each type, and even subtype of cancer, has different genetic, epigenetic, and other cellular events responsible for malignant development and metastasis. Recent work has indicated that microRNAs (miRNAs) play a major role in these processes, sometimes by promoting cancer growth and other times by suppressing tumorigenesis. miRNAs are small, noncoding RNAs that negatively regulate expression of specific target genes. This review goes into an in‐depth look at the most recent finding regarding the significance of one particular miRNA, miR‐146a‐5p, and its involvement in cancer. Target gene validation and pathway analysis have provided mechanistic insight into this miRNA's purpose in assorted tissues. Additionally, this review outlines novel findings that suggest miR‐146a‐5p may be useful as a noninvasive biomarker and as a targeted therapeutic in several cancers. This article is categorized under: RNA in Disease and Development > RNA in Disease Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs

show abstract

“…Alternatively, a miRNA binding of a target transcript can result in translational up-regulation [ 34 ] within their mRNA resulting in inhibition of translation or mRNA degradation [ 35 ]. Overall, miRNAs are recognized as potent regulators of eukaryotic gene expression at the post-transcriptional level [ 36 , 37 ] that regulate cell differentiation [ 38 ], migration [ 39 ], and neuronal development [ 40 ]. Approximately 256 D .…”

Section: Introductionmentioning

confidence: 99%

Differentially expressed microRNAs associated with changes of transcript levels in detoxification pathways and DDT-resistance in the Drosophila melanogaster strain 91-R

Seong¹,

Coates²,

Kim³

et al. 2018

PLoS ONE

View full text Add to dashboard Cite

Dichloro-diphenyl-trichloroethane (DDT) resistance among arthropod species is a model for understanding the molecular adaptations in response to insecticide exposures. Previous studies reported that DDT resistance may involve one or multiple detoxification genes, such as cytochrome P450 monooxygenases (P450s), glutathione S-transferases (GSTs), esterases, and ATP binding cassette (ABC) transporters, or changes in the voltage-sensitive sodium channel. However, the possible involvement of microRNAs (miRNAs) in the post-transcriptional regulation of genes associated with DDT resistance in the Drosophila melanogaster strain 91-R remains poorly understood. In this study, the majority of the resulting miRNAs discovered in small RNA libraries from 91-R and the susceptible control strain, 91-C, ranged from 16–25 nt, and contained 163 precursors and 256 mature forms of previously-known miRNAs along with 17 putative novel miRNAs. Quantitative analyses predicted the differential expression of ten miRNAs between 91-R and 91-C, and, based on Gene Ontology and pathway analysis, these ten miRNAs putatively target transcripts encoding proteins involved in detoxification mechanisms. RT-qPCR validated an inverse correlation between levels of differentially-expressed miRNAs and their putatively targeted transcripts, which implies a role of these miRNAs in the differential regulation of detoxification pathways in 91-R compared to 91-C. This study provides evidence associating the differential expression of miRNAs in response to multigenerational DDT selection in Drosophila melanogaster and provides important clues for understanding the possible roles of miRNAs in mediating insecticide resistance traits.

show abstract

MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer

Cited by 35 publications

References 36 publications

miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4

miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4

miR‐146a‐5p: Expression, regulation, and functions in cancer

Differentially expressed microRNAs associated with changes of transcript levels in detoxification pathways and DDT-resistance in the Drosophila melanogaster strain 91-R

Contact Info

Product

Resources

About