MicroRNA-153 Inhibits Osteosarcoma Cells Proliferation and Invasion by Targeting TGF-β2

Abstract

Increasing evidence indicates that microRNAs (miRNAs), a class of small noncoding RNAs, participate in almost every step of cellular processes. MiRNAs are aberrantly expressed in human cancers and contribute to cancer development and progression. Study of miRNAs may provide a new clue for understanding the mechanism of carcinogenesis and a new tool for cancer treatment. In the present study, miR-153 was downregulated in human osteosarcoma tissues and cell lines. Introduction of miR-153 mimics into the MG-63 cells inhibited cell proliferation and invasion. Our results further revealed that transforming growth factor beta 2 (TGF-β2) was negatively regulated by miR-153. Furthermore, overexpression of miR-153 decreased p-SMAD2, p-SMAD3, epidermal growth factor receptor (EGFR) and insulin-like growth factor binding protein-3 (IGFBP-3) expressions, which were the downstream signaling molecules of TGF-β. Furthermore, miRNA-153 suppressed TGF-β-mediated MG-63 proliferation and migration. Therefore, our results suggest that miR-153 may act as a tumor suppressor in osteosarcoma through targeting TGF-β2.

MicroRNA‐153‐3p regulates cell proliferation and cisplatin resistance via Nrf‐2 in esophageal squamous cell carcinoma

“…2 Some studies have found that miR-153-3p can inhibit the proliferation and invasive growth of breast cancer and osteosarcoma cells. 9,10 These findings indicate that miR-153-3p can act as a tumor suppressor and may serve as a potential target for the treatment of malignant tumors. However, whether miR-153-3p regulates the proliferation of ESCC cells and confers sensitivity to cisplatin chemotherapy remains unclear.…”

“…Upregulation of miR‐153 has been shown to inhibit the migration and invasion of ESCC cells, both in vitro and in vivo . Some studies have found that miR‐153‐3p can inhibit the proliferation and invasive growth of breast cancer and osteosarcoma cells . These findings indicate that miR‐153‐3p can act as a tumor suppressor and may serve as a potential target for the treatment of malignant tumors.…”

The anti-fibrotic effects of microRNA-153 by targeting TGFBR-2 in pulmonary fibrosis

“…It has been shown to participate in the pathogenesis of neurodegenerative diseases, such as Parkinson's and Alzheimer's disease (Liang et al, 2012). Moreover, it was reported to inhibit the proliferation and invasion of many types of malignant cells, for example osteosarcoma and lung cancer cells (Niu et al, 2015). Thus, it is viewed as a tumor suppressor.…”

MicroRNA‑153 affects nasopharyngeal cancer cell viability by targeting TGF‑β2

“…In the present study, it was identified that miR-153 upregulation significantly suppressed TGF-β 2 and Smad2 protein expression of 13-9B cells. Niu et al (23) suggested that miR-153 inhibits osteosarcoma cell viability and invasion through targeting TGF-β 2 . Liang et al (24) also identified that miR-153 disturbs TGF-β 1 /p-SMAD2/3 signal transduction, acting as an anti-fibrotic element in the development of pulmonary fibrosis.…”