MicroRNA‑153 suppresses human laryngeal squamous cell carcinoma migration and invasion by targeting the SNAI1 gene

Abstract

Human laryngeal squamous cell carcinoma (LSCC) is a malignant cancer type. Epithelial-mesenchymal transition marker Snail family transcriptional repressor 1 (SNAI1) is associated with the occurrence, development, invasion and metastasis of numerous tumor types, such as lung, liver and ovarian cancer. Previous studies have indicated that microRNA-153 (miR-153) may serve as a novel tumor suppressor, which is involved in tumor metastasis; however, the role and clinical significance of miR-153 in LSCC are not fully understood. The aim of the present study was to determine the role of miR-153 in the growth and aggressiveness of LSCC cells. Bioinformatics prediction method, western blot analysis, Matrigel invasion assay and immunofluorescence were used to analyze whether SNAI1 can be regulated and controlled by miR-153 in LSCC cells. An inverse association between miR-153 and SNAI1 was observed in LSCC tissues. It was demonstrated that SNAI1 is a direct target of miR-153 in LSCC. In addition, the results indicated that miR-153 knockdown inhibited PCI-13 cell migration and invasion by targeting SNAI1, which may be a potential marker that can reflect the degree of malignancy in patients with LSCC. Furthermore, miR-153 knockdown decreased Twist family BHLH transcription factor 1 and metastasis-associated 1 family member 3 expression in LSCC cells. In conclusion, these data indicated that miR-153 regulates LSCC migration via the targeting of SNAI1 gene, which may be a potential predictor for patients with LSCC.

MicroRNA-625 inhibits cell invasion and epithelial–mesenchymal transition by targeting SOX4 in laryngeal squamous cell carcinoma

“…From The Cancer Genome Atlas (TCGA) database, a number of differentially expressed miRNAs in LSCC have been identified [11]. Several miRNAs, including miR-26a and miR-153, emerged as powerful regulators in LSCC, as indicated in recent studies [12,13]. One major finding of our study is that miR-625 was markedly decreased in LSCC tissue specimens and cell lines.…”

Increased expression of miR-153 predicts poor prognosis for patients with prostate cancer

“…Furthermore, miR-153 inhibited migration, invasion and epithelial-mesenchymal transition of breast cancer by regulating transforming growth factor beta (TGF-β) signaling pathway. Zhang et al [15] found that miR-153 suppressed human laryngeal squamous cell carcinoma migration and invasion by targeting the Snail family transcriptional repressor 1 (SNAI1) gene.…”

Interplay among SNAIL Transcription Factor, MicroRNAs, Long Non-Coding RNAs, and Circular RNAs in the Regulation of Tumor Growth and Metastasis

“…Another example of miRNA that is described as a direct regulator of SNAIL expression in plenty tumor types is miR-153. The downregulation of SNAIL by miR-153 suppresses human laryngeal squamous cell carcinoma migration and invasion [75], melanoma cells proliferation and invasion [76], esophageal squamous cell carcinoma progression [77], and gastric cancer metastasis [78]; regulates EMT in hepatocellular carcinoma [79]; and diminishes pancreatic ductal adenocarcinoma migration and invasion with miR-153 serving as a prognostic marker [80].…”

“…laryngeal squamous cell carcinoma [75] melanoma [76] esophageal squamous cell carcinoma [77] gastric cancer [78] hepatocellular carcinoma [79] pancreatic ductal adenocarcinoma [80] miR-182 breast cancer [88] miR-199a lung cancer [91] papillary thyroid carcinoma [93] miR-203 breast cancer [86] miR-204 gastric cancer [90] miR-211-5p renal cancer [98] miR-363 ovarian cancer [94] miR-410-3p breast cancer [87] miR-486-5p prostate cancer [97] miR-491-5p gastric cancer [89] miR-502-5p hepatocellular carcinoma [96] miR-940 lung cancer [92]…”