MicroRNA-187 regulates gastric cancer progression by targeting the tumor suppressor CRMP1

Lian, Ren; Li, Fang; Di, Maojun; Fu, Yingfeng; Hui, Yuanjian; Xiao, Gaochun; Sun, Qiang; Liu, Yanwei; Ren, Dan; Du, Xianjin

doi:10.1016/j.bbrc.2016.11.079

Cited by 16 publications

(8 citation statements)

References 26 publications

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“…Overexpression of miR-187 was previously found to promote the proliferation, migration, and invasion of gastric cancer cells by targeting tumor suppressor CRMP1 (38). Thus, telmisartan may exert its antitumor effects by inhibiting the expression of miRNAs.…”

Section: Discussionmentioning

confidence: 97%

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Antihypertensive drug telmisartan suppresses the proliferation of gastric cancer cells in vitro and in vivo

Fujita

Iwama

et al. 2020

Oncol Rep

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Gastric cancer is one of the most common malignancies diagnosed worldwide. Telmisartan, an angiotensin receptor blocker (ARB), suppresses the proliferation of cancer cells and the growth of tumors through an unknown mechanism. To identify the mechanism, the present study was designed to evaluate the effects of telmisartan on gastric cancer cell lines and tumors in vitro and in vivo and the associated signaling molecules were identified. It was shown here that telmisartan suppressed the proliferation of the cultured human gastric cancer cell lines MKN74, MKN1 and MKN45 as detected in the CCK-8 assay. In a mouse xenograft model of gastric cancer, telmisartan suppressed tumor growth by arresting the cell cycle at the G0/G1 phase through inhibition of the expression of cyclin D1, the catalytic subunit of cyclin dependent kinase 4 (CDK4), as well as the phosphorylation of the tumor suppressor retinoblastoma (pRb) protein as detected by western blotting. Notably, telmisartan did not induce apoptosis, as indicated by consistent levels of caspase-cleaved keratin 18 in MKN74 cells. Furthermore, telmisartan inhibited the phosphorylation of epidermal growth factor receptor (EGFR) and increased the levels of the angiogenesis-related protein tissue inhibitor of metalloproteinase-1 (TIMP-1). Analyses of microarrays revealed that telmisartan altered the expression of miRNAs in MKN74 cells. In conclusion, telmisartan suppressed the proliferation of human gastric cancer cells by inducing cell cycle arrest.

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Section: Discussionmentioning

confidence: 97%

“…miR-185-5p, which was found to be expressed at lower levels, is a reliable diagnostic biomarker of gastric cancer (37). In contrast, miR-187, which was expressed at lower levels in MKN74 cells and is overexpressed in gastric cancer tissues, is associated with factors that influence the malignant phenotype (38).…”

Section: Discussionmentioning

confidence: 99%

Antihypertensive drug telmisartan suppresses the proliferation of gastric cancer cells in vitro and in vivo

Fujita

Iwama

et al. 2020

Oncol Rep

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“…An increasing number of evidence shows that CRMP-1 could inhibit cancer cells growth and metastasis. 3,24,25 Previously, we demonstrated by RT-qPCR, WB, and immunohistochemistry (IHC) that CRMP-1 expression markedly increased in early-onset PE compared to normal pregnancy, which suggested that CRMP-1 may be involved in pathogenesis of earlyonset PE. 7 In clinical, PE can be divided into 2 types, early onset and late onset, by 34 weeks of gestation.…”

Section: Discussionmentioning

confidence: 99%

“…The MTT assay revealed that CRMP-1 overexpression could decrease the proliferative capacity of these 2 cell lines compared to that of controls (1.31 2A). Also, according to the colony formation assay, cells transfected with pFL-CRMP-1 had significantly reduced cell clones formation compared to that of control (92 [3] vs 124 [4]; 100 [2] vs 174 [5]; P < .05; Figure 2B and C).…”

Section: Collapsin Response Mediator Protein 1 Overexpression Inhibited Proliferation Migration and Invasion Of Trophoblast Cellsmentioning

confidence: 92%

“…2 Moreover, clinical studies have reported aberrant CRMP-1 expression correlates with the tumor cell proliferation, invasion, metastasis, and the occurrence and prognosis of many kinds of tumors, such as neuroblastoma, lung cancer, and esophageal cancer. [3][4][5][6] Although it has been shown that CRMP-1 is expressed in trophoblast during gestation, especially increasing expression in the early-onset preeclamptic placenta, 7 its role in normal placental development and in the pathogenesis of the preeclampsia (PE) remains elusive.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion

Huang

Wang

et al. 2019

Reprod. Sci.

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Collapsin response mediator protein 1 (CRMP-1) is widely expressed in the nervous system and has tumor suppressive effects. Our previous studies have demonstrated that CRMP-1 was expressed in the trophoblasts of the whole stage of pregnancy with significantly increasing expression in the placenta of early-onset preeclampsia. Preeclampsia, especially early onset, is strongly associated with the dysfunction of trophoblast including proliferation, apoptosis, migration, and invasion. In this study, we found an inhibitory effect of CRMP-1 on proliferation, migration, invasion, and an enhanced effect on apoptosis in human trophoblast cell lines HTR-8/SVneo and JEG-3 by MTT assay, colony formation assay, cell viability assay, caspase 3/7 activity assay, scratch wound assay, and Matrigel Transwell assay. Overexpression of CRMP-1 in trophoblast cells led to downregulate expression of matrix metalloproteinase 2 and 9. The expression of CRMP-1 was detected by real-time quantitative polymerase chain reaction and Western blot analysis. Thus, we suggested that CRMP-1 might have implications for the pathogenesis of preeclampsia by regulating the biological behavior of trophoblast cells.

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Integrated Analysis of Serum and Tissue microRNA Transcriptome for Biomarker Discovery in Gastric Cancer

Wang,

Li,

Zhang

2024

Environmental Toxicology

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Gastric cancer (GC) poses a significant global health challenge, demanding a detailed exploration of its molecular landscape. Studies suggest that exposure to environmental pollutants can lead to changes in microRNA (miRNA) expression patterns, which may contribute to the development and progression of GC. MiRNAs have emerged as crucial regulators implicated in GC pathogenesis. The largest GC serum miRNA dataset to date, comprising 1417 non‐cancer controls and 1417 GC samples was used. We conducted a comprehensive analysis of miRNA expression profiles. Differential expression analysis, co‐expression network construction, and machine learning models were employed to identify key serum miRNAs and their association with clinical parameters. Weighted Gene Co‐expression Network Analysis (WGCNA) and immune infiltration analysis were used to validate the importance of the key miRNA. A total of 1766 differentially expressed miRNAs were identified, with miR‐1290, miR‐1246, and miR‐451a among the top up‐regulated, and miR‐6875‐5p, miR‐6784‐5p, miR‐1228‐5p, and miR‐6765‐5p among the top down‐regulated. WGCNA revealed that modules M1 and M5 were significantly associated with GC subtypes and disease status. MiRNA‐target gene network analysis identified prognostically significant genes TP53, EMCN, CBX8, and ALDH1A3. Machine learning models LASSO, SVM, randomforest, and XGBOOST demonstrated the diagnostic potential of miRNA profiles. Tissue and serum miR‐187 emerged as an independent prognostic factor, influencing patient survival across clinical parameters. Gene expression and immune cell infiltration were different in tissues stratified by miR‐187 expression. In summary, the integration of differential gene expression, co‐expression analysis, and immune cell profiling provided insights into the molecular intricacies of GC progression.

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MicroRNA-187 regulates gastric cancer progression by targeting the tumor suppressor CRMP1

Cited by 16 publications

References 26 publications

Antihypertensive drug telmisartan suppresses the proliferation of gastric cancer cells in vitro and in vivo

Antihypertensive drug telmisartan suppresses the proliferation of gastric cancer cells in vitro and in vivo

Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion

Integrated Analysis of Serum and Tissue microRNA Transcriptome for Biomarker Discovery in Gastric Cancer

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