MicroRNA-191 targets <italic>N</italic>-deacetylase/<italic>N</italic>-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803

Abstract

As a family of post-transcriptional regulator of gene expression, the microRNAs (miRNAs) control a wide array of biological processes including cell differentiation, proliferation and apoptosis, and the dysregulation of miRNAs is a hallmark of cancer. Here, we found that the was at a high-expression level in human gastric adenocarcinoma cell line MGC803 and human gastric cancer tissues. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays showed that miR-191 could promote cell growth and suppress apoptosis of MGC803 cells. The N-deacetylase/N-sulfotransferase 1 (NDST1) was confirmed to be a direct target gene of miR-191 by enhanced green fluorescent protein reporter experiment. The mRNA and protein levels of NDST1 were inversely correlated with miR-191 in MGC803 cells, suggesting the negative regulation of NDST1 by miR-191. Furthermore, NDST1 played an inhibitory role and could suppress MGC803 cell proliferation. Our findings suggested that miR-191 could act as an oncogene in MGC803 cells, and the cellular function was partially due to its negative regulation of NDST1.

MicroRNA-191 correlates with poor prognosis of colorectal carcinoma and plays multiple roles by targeting tissue inhibitor of metalloprotease 3

“…Overexpression of miR-191 has been observed in gastric carcinoma, hepatocellular carcinoma and colorectal carcinoma, implying its important role in tumorigenesis [12][13][14]. In this study, we found that high expression of miR-191 correlated with TNM stage as well as lymph node and liver metastasis, strongly suggesting that miR-191 might be involved in progression and metastasis of colorectal cancer.…”

“…Nine candidate target genes of miR-191 have been identified in hepatocellular carcinoma [16], and N-deacetylase/N-sulfotransferase 1 (NDST1) was confirmed to be a direct target gene of miR-191 in gastric cancer [14]. Here, we demonstrated that a target of miR-191 in CRC was TIMP3, which was also a candidate target gene of miR-191 in hepatocellular carcinoma [16].…”

“…In CRC, several miRNAs have been found to correlate with prognosis of patients [8][9][10]. Previous studies reported that miR-191 was upregulated in CRC [11,12] and it could enhance cell proliferation, decreases apoptosis in hepatocellular carcinoma and in gastric carcinoma [13,14]. However, the role of miR-191 in CRC progress and the relationship between the expression of miR-191 and prognosis in patients remained unclear.…”

MicroRNA‑191‑5p exerts a tumor suppressive role in renal cell carcinoma

“…Recently, miR-191-5p has been revealed to promote pancreatic cancer progression by targeting ubiquitin specific peptidase 10 (33). Shi et al (34) reported that miR-191-5p exhibits a promotive effect by targeting N-deacetylase and N-sulfotransferase 1 in MGC803 human gastric cancer cells. In a lung cancer research, Xu et al (35) demonstrated that miR-191-5p positively modulated the epithelial-mesenchymal transition and cancer stem cell-like properties and functioned as an onco-miR in transfected cells.…”

Loss of SNAIL Regulated miR-128-2 on Chromosome 3p22.3 Targets Multiple Stem Cell Factors to Promote Transformation of Mammary Epithelial Cells

“…1A) were amplified in breast cancer compared with normal breast tissues. miR-191 has been validated to be amplified and is viewed as a candidate oncogenic miRNA in liver (38), stomach (39), and pancreas (40). LOH comprises not only monosomy, caused by loss of 1 allele, but also homozygous disomy and homozygous polysomies, caused by the concurrent loss of 1 allele and endomultiplication of the other allele (41).…”