MicroRNA-194 promotes osteoblast differentiation via downregulating STAT1

Li, Jun; He, Xijing; Wei, Wenzhi; Zhou, Xiaobo

doi:10.1016/j.bbrc.2015.03.059

Cited by 63 publications

(39 citation statements)

References 44 publications

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“…miR‐194 has been reported to act a great role in numerous physiological and pathological pathways. For instance, overexpression of miR‐194 can decrease stellate cell activation (Venugopal et al, ), modulate hepatocytic differentiation of progenitor cells (Jung et al, ) and osteoblast differentiation (Li et al, ). In addition, miR‐194 is involved in Toll‐Like Receptor 4 Inflammatory Responses in THP‐1 Cells (Tian et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…A growing body of studies have identified that various miRNAs are involved in the ageing process and cellular senescence (Dimri et al, 2013). is one of the p53 responsive miRNAs (Pichiorri et al, 2010), which has been reported to exert an important role in the regulation of cellular differentiation (Jung et al, 2016a;Li et al, 2015), epithelial-mesenchymal transition (EMT) Li et al, 2014), and inflammatory responses (Tian et al, 2015). Moreover, miR-194 significantly suppresses the proliferation and invasion of diverse cancer cells, and thus functions as a typical tumor suppressor (Han et al, 2014;Zhu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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miR‐194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts

Zhang

Zhu

et al. 2017

Cell Biology International

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MicroRNA-194 (miR-194), a typical p53 responsive miRNA, serves as a tumor suppressor similar as p53, and has been demonstrated to play an anti-proliferation role in various human cancers. In spite of the pivotal role of p53 during aging process, the knowledge of miR-194's contribution to cellular senescence is limited. We herein sought to explore the role of miR-194 in the replicative senescence and stress-induced senescence of mouse embryonic fibroblasts. Our results unraveled that, compared to young cells, miR-194 is highly expressed in senescent cells, and extra expression of miR-194 significantly triggers the replicative senescence of MEFs and H O -induced senescence of NIH/3T3 cells, while inhibition of miR-194 exhibited the opposite effect. We further unveiled that DNMT3A was a direct and authentic target of miR-194, which has been reported to be closely associated with cellular senescence. Taken together, our data suggest that miR-194 may significantly promote the development of cellular senescence in mouse embryonic fibroblasts, which potentially occurs through inhibiting the DNMT3A expression.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

miR‐194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts

Zhang

Zhu

et al. 2017

Cell Biology International

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“…For instance, miR‐690 was found to regulate osteogenic differentiation of C2C12 cells through targeting nuclear factor‐kappaB p65 . Besides, miR‐144‐3p regulates C3H10T1/2 cells through modulating Smad4, miR‐194 modulates osteoblast differentiation by targeting signal transducer and activator of transcription 1‐mediated runt‐related gene 2 nuclear translocation, and miR‐214 has a crucial role in inhibiting bone formation by directly targeting activating transcription factor 4 . The Dkk‐1‐regulated Wnt/β‐catenin pathway is of great importance both in osteoblasts and periodontal tissues, and is reported to be regulated by miR‐9, miR‐29a, and miR‐335‐5p …”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐3064‐3p regulates the differentiation of cementoblasts through targeting DKK1

Wang

Liao

Sun

et al. 2018

J of Periodontal Research

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“…STAT1 inhibits osteoblast differentiation by blocking Runx2 nuclear translocation (18). Targeting the inhibition of STAT1 by various agents showed a promotive effect on osteoblast differentiation (43)(44)(45)(46). In this study, we demonstrated that inhibition of STAT1 by miR-10b suppression-induced Bcl6 promoted Runx2 nuclear translocation and osteoblast differentiation, indicating a potential strategy for the control of osteoblast differentiation by targeting STAT1.…”

Section: Discussionmentioning

confidence: 57%

Downregulation of miR-10b promotes osteoblast differentiation through targeting Bcl6

Yang

Wang

et al. 2017

International Journal of Molecular Medicine

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MicroRNAs (miRNAs or miRs) have been shown to play a critical role in osteoblast differentiation. miR-10b has been found to be downregulated during osteoblast differentiation; however, its precise effect on osteoblast differentiation remains unknown. In this study, we aimed to investigate the potential role of miR-10b and the potential underlying mechanism in regulating osteoblast differentiation. We found that miR-10b was downregulated during osteoblast differentiation. Overexpression of miR-10b inhibited osteoblast differentiation, whereas the suppression of miR-10b promoted osteoblast differentiation. Bioinformatics analysis and the dual-luciferase reporter assay demonstrated that miR-10b could target the 3'-untranslated regions of B cell lymphoma 6 (Bcl6) which is an important regulator of osteoblast differentiation. Real‑time quantitative polymerase chain reaction and western blot analysis showed that miR-10b directly regulated Bcl6 expression. Further experiments showed that the overexpression of miR-10b increased the expression of signal transducer and activator of transcription 1 (STAT1) and blocked Runt-related transcription factor 2 (Runx2) nuclear translocation, whereas miR-10b suppression showed an opposite effect. Moreover, the miR-10b suppression-induced effects were partially reversed by Bcl6 knockdown. Taken together, our study suggests that miR-10b contributes to osteoblast differentiation through targeting Bcl6, providing a novel insight into understanding the molecular mechanism underlying osteoblast differentiation and suggesting a potential target for inhibiting bone loss.

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MicroRNA-194 promotes osteoblast differentiation via downregulating STAT1

Cited by 63 publications

References 44 publications

miR‐194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts

miR‐194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts

MicroRNA‐3064‐3p regulates the differentiation of cementoblasts through targeting DKK1

Downregulation of miR-10b promotes osteoblast differentiation through targeting Bcl6

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