MicroRNA-194 Regulates Lipopolysaccharide-Induced Cell Viability by Inactivation of Nuclear Factor-κ B Pathway

Xie, Fei; Yang, Lei; Han, Lili; Yue, Bin

doi:10.1159/000484453

Cited by 11 publications

(9 citation statements)

References 33 publications

(32 reference statements)

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“…Prior findings have indicated that miR-194-5p regulates apoptosis and genes targeted by miR-194-5p are enriched in apoptotic and inflammatory cell pathways, such as MAPK (Wang et al, 2019). The overexpression of miR-194 has been shown to alleviate cellular apoptosis in LPS-induced ALI (Xie et al, 2017). Forkhead box P2 (FOXP2), a transcription factor, is involved in chromatin remodeling, which regulates the transcription of downstream genes (Bruce and Margolis, 2002).…”

Section: Introductionmentioning

confidence: 99%

Knockdown of lncRNA MALAT1 Alleviates LPS-Induced Acute Lung Injury via Inhibiting Apoptosis Through the miR-194-5p/FOXP2 Axis

Nan

Zhang

Cheung

et al. 2020

Front. Cell Dev. Biol.

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Purpose: We aimed to identify and verify the key genes and lncRNAs associated with acute lung injury (ALI) and explore the pathogenesis of ALI. Research showed that lower expression of the lncRNA metastasis-associated lung carcinoma transcript 1 (MALAT1) alleviates lung injury induced by lipopolysaccharide (LPS). Nevertheless, the mechanisms of MALAT1 on cellular apoptosis remain unclear in LPS-stimulated ALI. We investigated the mechanism of MALAT1 in modulating the apoptosis of LPS-induced human pulmonary alveolar epithelial cells (HPAEpiC). Methods: Differentially expressed lncRNAs between the ALI samples and normal controls were identified using gene expression profiles. ALI-related genes were determined by the overlap of differentially expressed genes (DEGs), genes correlated with lung, genes correlated with key lncRNAs, and genes sharing significantly high proportions of microRNA targets with MALAT1. Quantitative real-time PCR (qPCR) was applied to detect the expression of MALAT1, microRNA (miR)-194-5p, and forkhead box P2 (FOXP2) mRNA in 1 µg/ml LPS-treated HPAEpiC. MALAT1 knockdown vectors, miR-194-5p inhibitors, and ov-FOXP2 were constructed and used to transfect HPAEpiC. The influence of MALAT1 knockdown on LPS-induced HPAEpiC proliferation and apoptosis via the miR-194-5p/FOXP2 axis was determined using Cell counting kit-8 (CCK-8) assay, flow cytometry, and Western blotting analysis, respectively. The interactions between MALAT1, miR-194-5p, and FOXP2 were verified using dualluciferase reporter gene assay. Results: We identified a key lncRNA (MALAT1) and three key genes (EYA1, WNT5A, and FOXP2) that are closely correlated with the pathogenesis of ALI. LPS stimulation promoted MALAT1 expression and apoptosis and also inhibited HPAEpiC viability. MALAT1 knockdown significantly improved viability and suppressed the apoptosis of LPS-stimulated HPAEpiC. Moreover, MALAT1 directly targeted miR-194-5p, a downregulated miRNA in LPS-stimulated HPAEpiC, when FOXP2 was overexpressed.

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Section: Introductionmentioning

confidence: 99%

Knockdown of lncRNA MALAT1 Alleviates LPS-Induced Acute Lung Injury via Inhibiting Apoptosis Through the miR-194-5p/FOXP2 Axis

Nan

Zhang

Cheung

et al. 2020

Front. Cell Dev. Biol.

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“…A previous study showed that miR-194 expression was markedly decreased in A549 alveolar epithelial cells following infection with influenza A virus (IAV), and miR-194 could suppress fibroblast growth factor 2 (FGF2) expression at the mRNA and protein levels 89 . Xie and colleagues suggested that miR-194 increases cell apoptosis by inhibiting the NF- κ B pathway in WI38 cells, and it may be used as a potential targeted therapy for the treatment of infantile pneumonia 90 . In this present study, miR-92-x had a sharp down-regulation, while miR-194-y was significantly up-regulated in the A. c. cerana midgut 10 dpi with N. ceranae .…”

Section: Discussionmentioning

confidence: 99%

Comparative identification of microRNAs inApis cerana ceranaworkers’ midguts responding toNosema ceranaeinvasion

Guo

Chen

et al. 2019

Preprint

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30 MicroRNAs (miRNAs) are endogenous small noncoding RNAs that post 31 transcriptionally regulate gene expression and are involved in many biological 32 processes including host-pathogen interactions. However, the potential role of 33 miRNAs in the responses of eastern honeybees to Nosema ceranae invasion is 34 completely unknown. Here, the expression profiles and differentially expressed 35 miRNAs (DEmiRNAs) in the midguts of Apis cerana cerana workers 7 and 10 days 36 post infection (dpi) with N. ceranae were investigated via small RNA sequencing and 37 bioinformatics. In total, 529 miRNAs highly conserved between various species and 38 25 novel miRNAs with varied expressions were identified for the first time. In 39 addition, stem-loop RT-PCR confirmed the expression of 16 predicted miRNAs, 40 validating their existence. Eight up-regulated miRNAs and six down-regulated 41 miRNAs were detected in midguts at 7 dpi, while nine and three miRNAs were 42 significantly up-regulated and down-regulated, respectively, in midguts at 10 dpi. In 43 addition, Venn analysis showed that five DEmiRNAs were shared, while nine and 44 seven DEmiRNAs were specifically expressed in midguts at 7 and 10 dpi, 45 respectively. Gene ontology analysis suggested that a portion of the DEmiRNAs and 46 corresponding target genes were involved in various biological processes, cellular 47 components, and molecular functions including immune system processes and 48 response to stimulus and signaling. Moreover, KEGG pathway analysis shed light on 49 the potential functions of some DEmiRNAs in the regulation of target genes engaged 50 in material and energy metabolism, cellular immunity such as endocytosis and 51 phagosome, and the humoral immune system, including the Jak-STAT and MAPK 52 signaling pathways. Further investigation demonstrated a complex regulation network 53 between DEmiRNAs and their target mRNAs, with miR-598-y, miR-252-y, miR-92-x 54 and miR-3654-y at the center of the network, implying their key parts in host 55 responses. This comprehensive miRNA transcriptome analysis demonstrated that N. 56 ceranae invasion influenced the expression of miRNAs in the midguts of A. c. 57 ceranae workers; the results can not only facilitate future exploration of the regulatory 58 roles and mechanisms of miRNAs in hosts' responses, especially their immune 59 3 responses to N. ceranae, but also provide potential candidates for further investigation 60 of the molecular mechanisms underlying eastern honeybee-microsporidian 61 interactions. 62

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“…Therapeutics that target the NF-κB family can inhibit its translocation during inflammation in a process termed trans -repression and can protect against the condition of pulmonary and airway obstruction-associated inflammation ( 23 , 39 ). Several studies have described the therapeutic implications on lung diseases and its pathogenesis by inhibiting the NF-κB pathway through microRNA-194 in infantile pneumonia ( 42 ), and curcumin and resveratrol combination in MRC-5 cells for idiopathic pulmonary fibrosis ( 28 ). The suppression of NF-κB in inflammatory diseases by natural compounds derived from plants have been reported for inflammatory diseases by inhibiting its downstream targets viz., and inflammatory mediators iNOS, COX-2 and PGE2 ( 43 - 47 ).…”

Section: Discussionmentioning

confidence: 99%

Essential oil from Korean Chamaecyparis obtusa leaf ameliorates respiratory activity in Sprague‑Dawley rats and exhibits protection from NF-κB-induced inflammation in WI38 fibroblast cells

et al. 2018

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To date, Korean hinoki cypress (Chamaecyparis obtusa), has been widely used for household and commercial purposes. Although the medicinal efficacy of hinoki cypress essential oil has been observed, that of the essential oil-derived terpenes, which exhibit a mechanism that acts against lung inflammation, remains to be fully elucidated. The present study investigated the anti-inflammatory effect of hinoki cypress leaf extracted essential oil on lipopolysaccharide (LPS)-stimulated WI38 fibroblast cells by inhibiting the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) pathway, which exhibited lung tissue protection through the olfactory administration of essential oil in Sprague-Dawley rats. GC/MS analysis derived 24 terpenes from the essential oil. The morphological observations revealed that, upon LPS stimulation of WI38 fibroblast cells, inflammation was induced, whereas the condition of the cells reverted to normal in the essential oil extract pre-treated group. The results of western blot analysis revealed the inhibition of inducible nitric oxide synthase, activation of cyclooxygnase-2, and the degradation of cytosolic p65 and inhibitor of NF-κB-α in the LPS-stimulated group. Additionally, confocal imaging of nuclei revealed the translocation of phosphorylated p65, which was recovered in the cytosol in the phytoncide essential oil pre-treated group. Histopathological observation revealed that the alveolar capacity was enhanced in the essential oil olfactory administered rat group, compared with that in the normal rat group. These findings suggest that terpenes in essential oil from the Chamaecyparis obtusa leaf have therapeutic potential against respiratory inflammation-related disease.

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MicroRNA-194 Regulates Lipopolysaccharide-Induced Cell Viability by Inactivation of Nuclear Factor-κ B Pathway

Cited by 11 publications

References 33 publications

Knockdown of lncRNA MALAT1 Alleviates LPS-Induced Acute Lung Injury via Inhibiting Apoptosis Through the miR-194-5p/FOXP2 Axis

Knockdown of lncRNA MALAT1 Alleviates LPS-Induced Acute Lung Injury via Inhibiting Apoptosis Through the miR-194-5p/FOXP2 Axis

Comparative identification of microRNAs inApis cerana ceranaworkers’ midguts responding toNosema ceranaeinvasion

Essential oil from Korean Chamaecyparis obtusa leaf ameliorates respiratory activity in Sprague‑Dawley rats and exhibits protection from NF-κB-induced inflammation in WI38 fibroblast cells

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