microRNA-195 inhibits cell proliferation in bladder cancer via inhibition of cell division control protein 42 homolog/signal transducer and activator of transcription-3 signaling

Zhao, Cheng; Lin, Qi; Chen, Minfeng; Liu, Longfei; Yan, Wang‐Ji; Tong, Shiyu; Zu, Xiongbing

doi:10.3892/etm.2015.2633

Cited by 18 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study RT-qPCR analysis revealed that miR-195 was upregulated in RCC, whereas previous studies have shown that miR-195 is downregulated in the majority of types of cancer, including colorectal cancer (17), glioblastoma (18), bladder cancer (11,12), osteosarcoma (13), cervical cancer (14), gastric cancer (19), hepatocellular carcinoma (20), esophageal squamous cell carcinoma (10), breast cancer (21), non-small cell lung cancer (22) and prostate cancer (23). Therefore, the present study, to the best of our knowledge apoptotic rate of the ACHN cells with a characteristic low apoptotic rate.…”

Section: Discussioncontrasting

confidence: 75%

“…Therefore, in prostate cancer, miR-195 functions as a tumor suppressor partially by targeting BCOX1, Fra-1 and fibroblast growth factor 2 FGF2. In bladder cancer it has been demonstrated that miR-195 induces G1-phase arrest by targeting CDK4 (27), and inhibits bladder cancer cell proliferation, at least partially, through the inhibition of Cdc42/STAT3 signaling (12). miR-195 has been indicated to be associated with the glycometabolism in bladder cancer by suppressing glucose uptake through regulating the expression of GLUT3 (11).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that miRNAs are associated with various cellular processes, including proliferation, apoptosis, differentiation and stress response (6,10). miR-195, located on chromosome 17p13.1, has been shown to be downregulated and function as a tumor suppressor in different types of tumor, including bladder cancer (11,12), osteosarcoma (13) and cervical cancer (14). However, previous miRNA microarray chip analysis of RCC showed that miR-195-3p, the mature sequence of miR-195 also termed miR-195, was upregulated (15), which revealed that the role of miR-195-3p in RCC may be different, compared with other tumors.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of miR-195-3p as an oncogene in RCC

Jin¹,

Li²,

Li³

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

There is increasing evidence that the deregulation of microRNAs (miRNAs; miRs) contributes to tumorigenesis. Previous studies have shown that miR‑195 is downregulated in various types of cancer. The present study aimed to investigate the function and expression levels of miR‑125b. Results of qPCR revealed that miR‑195‑3p, the mature sequence of miR‑195, was upregulated in renal cell carcinoma (RCC) tissues and cell lines (786‑O, 769P and ACHN). This indicated that the function and role of miR‑195‑3p may differ in different types of tumor. To assess the function of miR‑195‑3p in RCC cell lines, cell proliferation was examined using MTT and CCK‑8 assays, mobility was assessed using a cell scratch assay, Transwell migration assay and invasion assay, and apoptosis was examined using flow cytometry. These assessments were also performed in cells with upregulated or downregulated miR‑195‑3p via transfection with synthesized miR‑195‑3p mimic or inhibitor. The results revealed that the overexpression of miR‑195‑3p promoted 786‑O and ACHN RCC cell proliferation, migration and invasion, and inhibited cell apoptosis, whereas the downregulation of miR‑195‑3p suppressed cell proliferation, migration and invasion, and induced cell apoptosis. These results indicated that miR‑195‑3p was associated with the tumorigenesis of RCC, with further investigations to focus on the pathway and use of miR‑195‑3p as a clinical biomarker for RCC.

show abstract

Section: Discussioncontrasting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of miR-195-3p as an oncogene in RCC

Jin¹,

Li²,

Li³

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

show abstract

“…A recent study also showed that miRNA-93 is upregulated in BC and is associated with tumor stage and lymph node metastasis (Jiang et al, 2019). And studies have reported that miRNA-195 inhibits glucose uptake and proliferation of BC T24 cells by inhibiting GLUT3 expression (Fei et al, 2012), another study found that miRNA-195 inhibits BC cell proliferation by inhibiting Cdc42 / STAT3 signaling (Zhao et al, 2015).…”

Section: Discussionmentioning

confidence: 97%

Identification of the Key Factors Related to Bladder Cancer by lncRNA-miRNA-mRNA Three-Layer Network

et al. 2020

View full text Add to dashboard Cite

Bladder cancer is the most common malignant tumor of the urinary system, and it has high incidence, high degree of malignancy, and easy recurrence after surgery. The etiology and pathogenesis of bladder cancer are not fully understood, but more and more studies have shown that its development may be regulated by some core molecules. To identify key molecules in bladder cancer, we constructed a three-layer network by merging lncRNA-miRNA regulatory network, miRNA-mRNA regulatory network, and lncRNA-mRNA coexpression network, and further analyzed the topology attributes of the network including the degree, betweenness centrality and closeness centrality of nodes. We found that miRNA-93 and miRNA-195 are controllers for a three-layer network and regulators of numerous target genes associated with bladder cancer. Functional enrichment analysis of their target mRNAs revealed that miRNA-93 and miRNA-195 may be closely related to bladder cancer by disturbing the homeostasis of the cell cycle or HTLV-I infection. In addition, since E2F1 and E2F2 are enriched in various KEGG signaling pathways, we conclude that they are important target genes of miRNA-93, and participate in the apoptotic process by forming a complex with a certain protein or transcription factor activity, sequence-specific DNA binding in bladder cancer. Similarly, AKT3 is an important target gene of miRNA-195, its expression is associated with PI3K-Akt-mTOR signaling pathway and AMPK-mTOR signaling pathway. Therefore, we speculate that AKT3 may participate in proliferation and apoptosis of bladder cancer cells through these pathways, and ultimately affect the biological behavior of tumor cells. Furthermore, through survival analysis, we found that miRNA-195 and miRNA-93 are associated with poor prognosis of bladder cancer. And the Kaplan-Meier curve showed that 24 mRNAs and nine lncRNAs are closely related to overall survival of bladder cancer.

show abstract

“…Previous studies had demonstrated that some miRNAs were associated with tumorigenesis and development of various cancers [7,15]. Due to the differences of target genes, these miRNAs could act as oncogenes or tumor suppressors in a various types of human tumors [16].…”

Section: Discussionmentioning

confidence: 99%

The Relationship Between microRNA-195 and the Prognosis of Cervical Cancer

Cao

Zheng

2020

Preprint

View full text Add to dashboard Cite

Background: MicroRNA-195 (miR-195), a tumor suppressor, had reported to be involved in carcinogenesis and the progression of some cancers. However, the prognostic value of miR-195 in cervical cancer remained unclear. The purpose of this study was to detect the expression of miR-195 in cervical cancer tissues and to investigate its correlation with tumor progression and prognosis.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the relative mRNA expression of miR-195 in cervical cancer tissues and corresponding adjacent normal tissues. The relationship between miR-195 expression and clinical characteristics of patients was analyzed by chi-square test. Kaplan-Meier method was applied to compare the overall survival, and the prognostic value of miR-195 was estimated via cox regression analysis.Results: Compared with normal tissues, miR-195 expression was significantly down-regulated in cervical cancer tissues (P < 0.001). Importantly, decreased expression of miR-195 was closely associated with FIGO stage, lymph node metastasis and vascular invasion (P < 0.05). Additionally, Kaplan-Meier analysis indicated that patients with high miR-195 expression had obviously longer overall survival than those with low miR-195 expression (log rank test, P = 0.001). And miR-195 was an independent prognostic factor of cervical cancer patients via univariate and multivariate cox regression analyses.Conclusions: Decreased expression of miR-195 is associated with the progression of cervical cancer. And miR-195 may have potency to predict the prognosis of cervical cancer.

show abstract

microRNA-195 inhibits cell proliferation in bladder cancer via inhibition of cell division control protein 42 homolog/signal transducer and activator of transcription-3 signaling

Cited by 18 publications

References 25 publications

Identification of miR-195-3p as an oncogene in RCC

Identification of miR-195-3p as an oncogene in RCC

Identification of the Key Factors Related to Bladder Cancer by lncRNA-miRNA-mRNA Three-Layer Network

The Relationship Between microRNA-195 and the Prognosis of Cervical Cancer

Contact Info

Product

Resources

About