MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10

You, Murong; Zhang, Liang; Zhang, Xiaoxiang; Fu, Yang; Xing, Dong

doi:10.2147/cia.s269171

Cited by 14 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KLF10 (Krüppel-like factor 10) has been established in several studies for its role as a tumor suppressor in cancer [ 62 ]. KLF10 has the potential to be a marker of various diseases, including cardiac hypertrophy [ 62 ], diabetes [ 63 ], osteoporosis [ 64 ], immune system diseases, and colitis [ 65 ]. KLF10 plays a significant role in many conditions and biological processes, including tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

FZD1/KLF10-hsa-miR-4762-5p/miR-224-3p-circular RNAs axis as prognostic biomarkers and therapeutic targets for glioblastoma: a comprehensive report

et al. 2023

View full text Add to dashboard Cite

Background The circular RNA (circRNA) plays a vital role in the pathogenesis of tumors as a competitive endogenous RNA (ceRNA). Given the high aggressiveness and fatality rate of glioblastoma (GBM) as well as poor prognosis, it is necessary to construct a circRNA-related ceRNA network for further studies on the mechanism of GBM and identify possible biomarkers as well as therapeutic drugs. Methods Three datasets from the gene expression omnibus (GEO) database were downloaded to distinguish differential circRNAs, microRNAs, and messenger RNAs respectively in GBM. With the help of GEPIA2, circBank, CSCD, TargetScan, miRDB, and miRTarBase databases, we established a circRNAs-related ceRNA network in GBM. Functional enrichments were employed to profile the most relevant mRNAs to indirectly clarify the mechanisms of the ceRNA network. Based on the expression profile data and survival information of GBM patients from the GEO and the cancer genome atlas (TCGA) databases, we performed survival analysis to select prognostic mRNAs and constructed a novel circRNA-miRNA-mRNA central regulatory subnetwork. The DGIdb database was used to find potential drug–gene interactions. Results The datasets obtained from the GEO and TCGA databases were analyzed, and 504 differentially expressed mRNAs (DEmRNAs), 71 differentially expressed microRNAs (DEmiRNAs), and 270 differentially expressed circRNAs (DEcircRNAs) were screened out. The novel ceRNA regulatory network included 22 circRNAs, 11 miRNAs, and 15 mRNAs. FZD1 and KLF10 were significantly correlated with the overall survival rate of patients with GBM (P < 0.05). The final survival subnetwork contained six circRNAs, two miRNAs, and two mRNAs. Two small-molecule compounds and one antibody could be used as therapeutic drugs for GBM. Interestingly, the Wnt signaling pathway appeared in both KEGG and GO functional terms. Conclusions Results of this study demonstrate that FZD1 and KLF10 may exert regulatory functions in GBM, and the ceRNA-mediated network could be a therapeutic strategy for GBM.

show abstract

Section: Discussionmentioning

confidence: 99%

FZD1/KLF10-hsa-miR-4762-5p/miR-224-3p-circular RNAs axis as prognostic biomarkers and therapeutic targets for glioblastoma: a comprehensive report

et al. 2023

View full text Add to dashboard Cite

show abstract

“…MiRNAs generally exert their regulatory role by selectively targeting protein-coding genes. Similarly, miR-197-3p has been shown to target multiple genes in human cells, such as KLF10 ( You et al,2021 ), ABCA7( Xie et al,2020 ) and EHD2 ( Fan et al,2020 ). However, TYMS as a target of miR-197-3p in any type of cancer cell has not been studied.…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Hub Genes and miRNA-mRNA Pairs Related to the Progression and Prognosis of Cervical Cancer Through Integrated Bioinformatics Analysis

Pei

Lü

et al. 2021

Front. Genet.

View full text Add to dashboard Cite

In recent years, the incidence and mortality of cervical cancer have increased worldwide. At the same time, increasing data have confirmed that miRNA-mRNA plays a positive or negative regulatory role in many cancers. This study attempted to screen effective miRNA-mRNA in the progression of cervical cancer, and to study the mechanism of miRNA-mRNA in the progression of cervical cancer. The expression profile data of GSE7410, GSE 63514, GSE 86100 and TCGA-CESC were downloaded, and 34 overlapping differentially expressed genes (22 up-regulated and 12 down-regulated) and 166 miRNAs (74 down-regulated and 92 up-regulated) were screened through limma package. Then, miR-197-3p/TYMS pairs were obtained by PPI, functional enrichment, Kaplan-Meier plotter analysis, Cox univariate and multivariate analysis, risk modeling, WGCNA, qPCR and dual-luciferase experiments. The results showed that TYMS was an independent prognostic factor of cervical cancer, and its expression level was negatively correlated with cervical cancer tissue grade (TMN), tumor grade, age, microsatellite stability and tumor mutation load, and positively correlated with methyl expression in DNMT1, DNMT2, DNMT3A and DNMT3B. Functional experiments showed that TYMS knockout could promote the proliferation, migration and invasion of HeLa cells and reduce apoptosis. Overexpression of TYMS showed the opposite trend, miR-197-3p was negatively correlated with the expression of TYMS. MiR-197-3p inhibitor reversed the effect of si-TYMS on the proliferation of HeLa cells. In conclusion, these results reveal that TYMS plays a very important role in the prognosis and progression of cervical cancer, and has the potential to be thought of as cervical cancer biomarkers. At the same time, miR-197-3p/TYMS axis can regulate the deterioration of cervical cancer cells, which lays a foundation for the molecular diagnosis and treatment of cervical cancer.

show abstract

“…In a rat model of ovariectomy-induced osteoporosis, silencing of miRNA-151a-3p decreased osteoclastogenesis-related factors and raised BMD ( He et al., 2021 ). miRNA-197-3p has been shown to inhibit osteogenic differentiation by downregulating KLF 10 in MC3T3-E1 cells ( You et al., 2021 ). In OP patients' femoral neck trabeculae, the expression level of miR-874-3p is quite low compared to healthy individuals, while the leptin level is high.…”

Section: Therapeutic Role Of Mirna In Several Bone Diseasesmentioning

confidence: 99%

Recent advancements of miRNAs in the treatment of bone diseases and their delivery potential

Sharma

Lee

2023

Current Research in Pharmacology and Drug Discovery

View full text Add to dashboard Cite

MicroRNA-197-3p Inhibits the Osteogenic Differentiation in Osteoporosis by Down-Regulating KLF 10

Cited by 14 publications

References 39 publications

FZD1/KLF10-hsa-miR-4762-5p/miR-224-3p-circular RNAs axis as prognostic biomarkers and therapeutic targets for glioblastoma: a comprehensive report

FZD1/KLF10-hsa-miR-4762-5p/miR-224-3p-circular RNAs axis as prognostic biomarkers and therapeutic targets for glioblastoma: a comprehensive report

Identification of Potential Hub Genes and miRNA-mRNA Pairs Related to the Progression and Prognosis of Cervical Cancer Through Integrated Bioinformatics Analysis

Recent advancements of miRNAs in the treatment of bone diseases and their delivery potential

Contact Info

Product

Resources

About