MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells

Gui, Rong; Huang, Rong; Zhang, Junhua; Wen, Xiang; Nie, Xinmin

doi:10.3892/or.2016.4550

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…Mir199a was found to be active in the inhibition of proliferation of HepG2 cells. This came in agreement with Gui et al (2016) who declared that forced expression of miR-199a-5p by a lentiviral vector expressing miR-199a-5p inhibited HepG2 cell proliferation as elucidated by decreased expression of Proliferating cell nuclear antigen (PCNA), a marker of tumor cell proliferation (Gui et al, 2016). Similar results were presented by Guo et al, (2017).…”

Section: Discussionsupporting

confidence: 85%

MicroRNA-199: A Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model

Atta

Kramani

Mohamed

et al. 2021

Asian Pac J Cancer Prev

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Section: Discussionsupporting

confidence: 85%

MicroRNA-199: A Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model

Atta

Kramani

Mohamed

et al. 2021

Asian Pac J Cancer Prev

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“…Our functional assays showed that ectopic expression of all members of the miR‐199 family inhibited cancer cell aggressiveness, indicating that the family acted as anti‐tumor miRNA in HNSCC cells. The anti‐tumor function of miR‐199a‐5p was reported in several types of cancers . With regard to miR‐199a‐5p , recent data showed that miR‐199a‐3p was downregulated in osteosarcoma and that restoration of miR‐199a‐3p significantly inhibited CD44 expression .…”

Section: Discussionmentioning

confidence: 99%

Regulation of ITGA3 by the anti‐tumor miR‐199 family inhibits cancer cell migration and invasion in head and neck cancer

et al. 2017

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For patients with head and neck squamous cell carcinoma (HNSCC), survival rates have not improved due to local recurrence and distant metastasis. Current targeted molecular therapies do not substantially benefit HNSCC patients. Therefore, it is necessary to use advanced genomic approaches to elucidate the molecular mechanisms underlying the aggressiveness of HNSCC cells. Analysis of our microRNA (miRNA) expression signature by RNA sequencing showed that the miR‐199 family (miR‐199a‐5p, miR‐199a‐3p, miR‐199b‐5p and miR‐199b‐3p) was significantly reduced in cancer tissues. Ectopic expression of mature miRNA demonstrated that all members of the miR‐199 family inhibited cancer cell migration and invasion by HNSCC cell lines (SAS and HSC3). These findings suggested that both passenger strands and guide strands of miRNA are involved in cancer pathogenesis. In silico database and genome‐wide gene expression analyses revealed that the gene coding for integrin α3 (ITGA3) was regulated by all members of the miR‐199 family in HNSCC cells. Knockdown of ITGA3 significantly inhibited cancer cell migration and invasion by HNSCC cells. Moreover, overexpression of ITGA3 was confirmed in HNSCC specimens, and high expression of ITGA3 predicted poorer survival of the patients (P = 0.0048). Our data revealed that both strands of pre‐miR‐199a (miR‐199a‐5p and miR‐199a‐3p) and pre‐miR‐199b (miR‐199b‐5p and miR‐199b‐3p) acted as anti‐tumor miRNA in HNSCC cells. Importantly, the involvement of passenger strand miRNA in the regulation of cellular processes is a novel concept in RNA research. Novel miRNA‐based approaches for HNSCC can be used to identify potential targets for the development of new therapeutic strategies.

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“…Other miRNAs such as miR-199a-5p and -125b-5p act as negative regulators of angiogenesis [29,30]. For instance, miR-199a-5p is known to inhibits VEGF-induced tumorigenesis [67] and suppresses human bladder cancer cell metastasis by targeting C-C chemokine receptor type 7 (CCR7) [68]; this miRNA is also documented to target ETS-1 and suppresses HMEC angiogenesis by inhibiting VEGF and HGF signaling [29]. miR-125b-5p expression inversely correlates with VEGF expression [30] and miR-125a are involved in the regulation of hematopoietic stem cell number [69].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA expression profile in retina and choroid in oxygen-induced retinopathy model

et al. 2019

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Background Ischemic retinopathies (IRs) are leading causes of visual impairment. They are characterized by an initial phase of microvascular degeneration and a second phase of aberrant pre-retinal neovascularization (NV). microRNAs (miRNAs) regulate gene expression, and a number play a role in normal and pathological NV. But, post-transcriptional modulation of miRNAs in the eye during the development of IRs has not been systematically evaluated. Aims & methods Using Next Generation Sequencing (NGS) we profiled miRNA expression in the retina and choroid during vasodegenerative and NV phases of oxygen-induced retinopathy (OIR). Results Approximately 20% of total miRNAs exhibited altered expression (up- or down-regulation); 6% of miRNA were found highly expressed in retina and choroid of rats subjected to OIR. During OIR-induced vessel degeneration phase, miR-199a-3p, -199a-5p, -1b, -126a-3p displayed a robust decreased expression (> 85%) in the retina. While in the choroid, miR-152-3p, -142-3p, -148a-3p, -532-3p were upregulated (>200%) and miR-96-5p, -124-3p, -9a-3p, -190b-5p, -181a-1-3p, -9a-5p, -183-5p were downregulated (>70%) compared to controls. During peak pathological NV, miR-30a-5p, -30e-5p and 190b-5p were markedly reduced (>70%), and miR-30e-3p, miR-335, -30b-5p strongly augmented (by up to 300%) in the retina. Whereas in choroid, miR-let-7f-5p, miR-126a-5p and miR-101a-3p were downregulated by (>81%), and miR-125a-5p, let-7e-5p and let-7g-5p were upregulated by (>570%) during NV. Changes in miRNA observed using NGS were validated using qRT-PCR for the 24 most modulated miRNAs. In silico approach to predict miRNA target genes (using algorithms of miRSystem database) identified potential new target genes with pro-inflammatory, apoptotic and angiogenic properties. Conclusion The present study is the first comprehensive description of retinal/choroidal miRNAs profiling in OIR (using NGS technology). Our results provide a valuable framework for the characterization and possible therapeutic potential of specific miRNAs involved in ocular IR-triggered inflammation, angiogenesis and degeneration.

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MicroRNA-199a-5p inhibits VEGF-induced tumorigenesis through targeting oxidored-nitro domain-containing protein 1 in human HepG2 cells

Cited by 10 publications

References 33 publications

MicroRNA-199: A Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model

MicroRNA-199: A Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model

Regulation of ITGA3 by the anti‐tumor miR‐199 family inhibits cancer cell migration and invasion in head and neck cancer

MicroRNA expression profile in retina and choroid in oxygen-induced retinopathy model

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