MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma

Sun, Haixiang; Yang, Zhixu; Tang, Wei‐Guo; Ke, Ai–Wu; Liu, Wei‐Ren; Li, Yan; Gao, Chao; Hu, Bo; Fu, Pei-Yao; Yu, Ming; Gao, Bowen; Shi, Ying‐Hong; Fan, Jia; Xu, Yang

doi:10.7150/jca.37748

Cited by 19 publications

(12 citation statements)

References 29 publications

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“…Both miR-19a-3p and miR-19a-5p have been reported to be crucial in the promotion or inhibition of cancer progression. For example, miR-19a-3p was reported to promote the proliferation of hepatocellular carcinoma cells via regulation of the PIK3IP1/AKT pathway ( 30 ); however, another study reported that miR-19-3p induces colorectal cancer cell apoptosis by repressing the expression of Fas cell surface death receptor ( 31 ). Furthermore, miR-19a-5p expression is significantly decreased in NSCLC, and miR-19a-5p can suppress the proliferation, migration and invasion of NSCLC cells ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2

Wang

Zhang

et al. 2021

Oncol Lett

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GATA6 antisense RNA 1 (GATA6-AS1) has been reported to be involved in the progression of several types of cancer. In the present study, the role of GATA6-AS1 in ovarian cancer (OC) was explored. Reverse transcription quantitative PCR was used to detect the expression of GATA6-AS1, microRNA (miR)-19a-5p and tet methylcytosine dioxygenase 2 (TET2) in OC and adjacent normal tissues. Furthermore, OC cells with GATA-AS1 either knocked down or overexpressed were established. The Cell Counting Kit-8 assay was used to evaluate cell proliferation and a Transwell assay was used to assess the migratory and invasive abilities of OC cells. A dual luciferase reporter gene assay was used to determine whether GATA6-AS1 and miR-19a-5p, and miR-19a-5p and TET2, may interact with each other. The results demonstrated that GATA6-AS1 expression level was decreased in OC tissues and cells compared with control groups. In addition, GATA6-AS1 overexpression significantly inhibited the proliferation and migratory and invasive abilities of OC cells, whereas GATA6-AS1 downregulation had the opposite effects. Furthermore, GATA6-AS1 adsorbed miR-19a-5p to repress its expression and GTA6-AS1 indirectly upregulated TET2 expression. Taken together, the findings from this study suggested that GATA6-AS1 could inhibit the proliferation and migratory and invasive abilities of OC cells via regulation of the miR-19a-5p/TET2 axis.

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Section: Discussionmentioning

confidence: 99%

GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2

Wang

Zhang

et al. 2021

Oncol Lett

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“…According to the previous publications, we have known that miRNAs drive the tumorigenesis of multiple cancers through regulating key genes expression. 20 Minichromosome maintenance genes exert a crucial role in DNA replication, including six genes MCM2-MCM7. 21 Liao et al analyzed distinct prognostic power and diagnostic value of MCM genes in HCC and found that MCM5 is significantly linked with the survival rate of HBV-induced HCC patients.…”

Section: Discussionmentioning

confidence: 99%

miR‐3607, a biomarker of hepatocellular carcinoma invasion and aggressiveness: Its relationship with epithelial‐mesenchymal transition process

Wei²,

Liu³

et al. 2020

IUBMB Life

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has been identified as an important biomarker, and its aberrant expression exerts a significant role in tumorigenesis. However, the biological function of miR-3607 in hepatocellular carcinoma (HCC) needs to be deciphered comprehensively. Clinical samples of HCC patients, as well as normal cases, were derived from The Cancer Genome Atlas database. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting analyses were utilized to detect the expression levels of indicated genes. Cell counting kit-8 (CCK-8), colony formation, and transwell assays were performed to assess the effect of miR-3607 in HCC cell viability, migration, and invasion. Bioinformatics analysis and luciferase reporter gene assay was applied to screen the target genes of miR-3607 and verified the association between miR-3607 and its potential target gene. Our study showed that miR-3607 expression was decreased in HCC tissues and cell lines, and its downregulation was linked with poor outcomes of HCC patients. miR-3607 was noted to inhibit HCC cell growth, colony formation, migration, and invasion. Besides, minichromosome maintenance (MCM5) was a possible target gene of miR-3607 in HCC. Overexpression of MCM5 was observed in HCC and induced unfavorable prognosis. MCM5 expression had a negative correlation with miR-3607. MCM5 can abolish the suppressive impacts of miR-3607 on HCC cell malignant behaviors and the epithelial-mesenchymal transition (EMT) process. To sum up, our results unveiled that miR-3607 could inhibit HCC cell growth, migration, and invasion by regulating MCM5 and mediating EMT process, suggesting a new probable biomarker for further treatment of HCC.

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“…Ultimately, 131 DEMs were identi ed, and of which four miRNAs such as miR-19a-3p, miR-144-3p, miR-223-5p, and miR-483-3p were associated with prognosis of PCa as the independent prognostic signatures in line with the Kaplan-Meier survival analysis, while the role of these DEMs in the progress of PCa is diverse depending on cancer types, which may act as oncogenes or tumor suppressors. MiR-19a-3p, as a key tumor-related miRNA of the miR-17-92 cluster (27), has been reported down-regulation in many types of cancers, including breast cancer (28), hepatocellular carcinoma (29), and glioma (30). MiR-19a-3p has been found high expression in primary PCa cell 22RV1, but was signi cantly decreased in bone metastatic PCa cell lines, including PC-3, C4-2B, and VCaP.…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of four miRNA prognostic signatures of prostate cancer

Chen

Yao

et al. 2020

Preprint

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Background Prostate cancer (PCa) is the most common urological cancer among men, having a poor prognosis, which is hard to accurately evaluate based on the present methods. MicroRNAs (miRNAs), a class of internal non-coding small RNA, can involve in the regulation of tumor biological function. So far, many researchers have tried to explore the relationship of malignant progress of PCa with miRNA, while there are just limited studies conducting the comprehensive analysis of miRNA in PCa clinical significance. Methods The data of miRNA and mRNA expressions in PCa were downloaded from TCGA database, and were performed the overall survival (OS) analysis using Survival package of R software to harvest the differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs). The bioinformatics tools such as TargetScan, miRDB, and miRanda were also conducted to forecast the desired target genes related with prognostic DEMs. In addition, both GO and KEGG analyses were used to uncover the fundamental signaling pathways and cellular processes in PCa as well as the protein-protein interaction (PPI) network was constructed through STRING and Cytoscape software. Results Firstly, 4 DEMs (miR-19a-3p, miR-144-3p, miR-223-5p, and miR-483-3p) were found having significantly associated with overall survival in PCa. Based on the criteria with FDR < 0.05 and |log2FC| > 1, 33 genes were screened out as DEGs. Besides, the functional enrichment analysis revealed that these DEGs of 4 miRNAs may participate in cancer-related pathways like FoxO and PI3K-Akt signaling pathway. Lastly, the low expression of CD177 may be potentially associated with poor survival of patients in PCa. Conclusion This study systematically analyzed multiple PCa prognostic DEMs (miR-19a-3p, miR-144-3p, miR-223-5p, and miR-483-3p), and verified a novel DEG signature (CD177) that can be used to effectively assess the prognosis of PCa patients.

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MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma

Cited by 19 publications

References 29 publications

GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2

GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2

miR‐3607, a biomarker of hepatocellular carcinoma invasion and aggressiveness: Its relationship with epithelial‐mesenchymal transition process

Integrative Analysis of four miRNA prognostic signatures of prostate cancer

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