MicroRNA-19b restricts Wnt7b to the hem, which induces aspects of hippocampus development in the avian forebrain

Sindhu, Suvimal Kumar; Udaykumar, Niveda; Zaidi, Mohd Ali Abbas; Soni, Abhinav; Sen, Jonaki

doi:10.1242/dev.175729

Cited by 5 publications

(7 citation statements)

References 28 publications

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“…E2f8 and Neurod1 (7,17). Now, to test the effect of miR-19b on E2f8 and Neurod1 mRNAs, we performed gain-offunction (GoF) and loss-of-function (LoF) experiments with miR-19b using expression construct for miR-19b and Sponge-19b, respectively (See materials and methods) (14), and found out that miR-19b is indeed sufficient to regulate the expression of E2f8 as well as Neurod1, whereas necessary for E2f8 expression but Neurod1 (Fig. 1H-K).…”

Section: Resultsmentioning

confidence: 99%

“…Further, to find out a cell-cycle regulator for this context, we used literature search and selected a microRNA, miR-19b that regulates proliferation in the mouse forebrain (13) and express in the chick pallial VZ at E4.5 (14). Our analysis showed miR-19b expression in the proliferating NPCs at VZ at E6.5 as well, as opposed to the Neurod1 in the SVZ (Fig.1E).…”

Section: Resultsmentioning

confidence: 99%

“…To identify candidate cell-cycle regulators in this context, based on literature search, we selected a microRNA, miR-19b, known to regulate cell proliferation in the mouse forebrain (13) and also is expressed in the VZ of the chick pallium at HH25 (14). Our analysis revealed that the expression domain of miR-19b in the VZ at HH30 is mutually exclusive from that of Neurod1 which is expressed in the SVZ (Fig.1E).…”

Section: Resultsmentioning

confidence: 99%

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Evidence of neocortex-like primitive laminar pallium in avian embryo and its organization by microRNA-19b

Sindhu

Mishra

Udaykumar

et al. 2020

Preprint

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The avian and mammalian brain contain similar cell types, but are organized quite differently. The dynamic changes in proliferation and differentiation of neuronal progenitors along the medial-lateral axis are among the unique features that distinguish the avian pallium from its mammalian counterpart. In this study carried out in the developing chick pallium, the analysis of expression pattern as well as functional studies of a microRNA, miR-19b, reveal that it is a potential regulator of the dynamics of neuronal progenitor proliferation and neuronal differentiation in this context. Moreover, was also found to be sufficient for regulating the expression of a lateral pallial marker, Mef2c. In summary, miR-19b is likely to be a pivotal player that shapes the developing avian pallium.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Evidence of neocortex-like primitive laminar pallium in avian embryo and its organization by microRNA-19b

Sindhu

Mishra

Udaykumar

et al. 2020

Preprint

Self Cite

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“…Aberrant expression of miR-19 in the hippocampus causes dysregulated migration of developing neurons ( Han et al, 2016 ). In addition, miR-19 plays a key role in the positioning of the hippocampal tissue via Wnt-mediated signaling pathway ( Sindhu et al, 2019 ). It has been demonstrated that miR-29a, in addition to regulate neural morphology and soma size, targets mRNAs related to extracellular matrix proteins including Fibrillin1, Follistatin-like1(FSTL1) and Laminin subunit gamma-2 to control neuronal migration ( Ma et al, 2020 ).…”

Section: Micrornas Are Important Regulators Of Neural Development In ...mentioning

confidence: 99%

The role of microRNAs in neurobiology and pathophysiology of the hippocampus

Rashidi,

Kalirad,

Rafie

et al. 2023

Front. Mol. Neurosci.

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MicroRNAs (miRNAs) are short non-coding and well-conserved RNAs that are linked to many aspects of development and disorders. MicroRNAs control the expression of genes related to different biological processes and play a prominent role in the harmonious expression of many genes. During neural development of the central nervous system, miRNAs are regulated in time and space. In the mature brain, the dynamic expression of miRNAs continues, highlighting their functional importance in neurons. The hippocampus, as one of the crucial brain structures, is a key component of major functional connections in brain. Gene expression abnormalities in the hippocampus lead to disturbance in neurogenesis, neural maturation and synaptic formation. These disturbances are at the root of several neurological disorders and behavioral deficits, including Alzheimer’s disease, epilepsy and schizophrenia. There is strong evidence that abnormalities in miRNAs are contributed in neurodegenerative mechanisms in the hippocampus through imbalanced activity of ion channels, neuronal excitability, synaptic plasticity and neuronal apoptosis. Some miRNAs affect oxidative stress, inflammation, neural differentiation, migration and neurogenesis in the hippocampus. Furthermore, major signaling cascades in neurodegeneration, such as NF-Kβ signaling, PI3/Akt signaling and Notch pathway, are closely modulated by miRNAs. These observations, suggest that microRNAs are significant regulators in the complicated network of gene regulation in the hippocampus. In the current review, we focus on the miRNA functional role in the progression of normal development and neurogenesis of the hippocampus. We also consider how miRNAs in the hippocampus are crucial for gene expression mechanisms in pathophysiological pathways.

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“…One of the key signaling centers in the mammalian CNS is the cortical hem (CH), which develops at the telencephalic dorsal midline, between the choroid plexus and cortical neuroepithelium ( Grove et al, 1998 ; Jones et al, 2019 ; Moore and Iulianella, 2021 ; Sindhu et al, 2019 ). The CH is necessary and sufficient for the development of the hippocampus in adjacent neuroepithelium ( Mangale et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Lmx1a is a master regulator of the cortical hem

Iskusnykh,

Fattakhov,

et al. 2023

eLife

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Development of the nervous system depends on signaling centers – specialized cellular populations that produce secreted molecules to regulate neurogenesis in the neighboring neuroepithelium. In some cases, signaling center cells also differentiate to produce key types of neurons. The formation of a signaling center involves its induction, the maintenance of expression of its secreted molecules, and cell differentiation and migration events. How these distinct processes are coordinated during signaling center development remains unknown. By performing studies in mice, we show that Lmx1a acts as a master regulator to orchestrate the formation and function of the cortical hem (CH), a critical signaling center that controls hippocampus development. Lmx1a co-regulates CH induction, its Wnt signaling, and the differentiation and migration of CH-derived Cajal–Retzius neurons. Combining RNAseq, genetic, and rescue experiments, we identified major downstream genes that mediate distinct Lmx1a-dependent processes. Our work revealed that signaling centers in the mammalian brain employ master regulatory genes and established a framework for analyzing signaling center development.

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MicroRNA-19b restricts Wnt7b to the hem, which induces aspects of hippocampus development in the avian forebrain

Abstract: In the Materials and Methods section, the service providers for in vitro Cas9 assay and Illumina Sequencing systems were not mentioned.

Cited by 5 publications

References 28 publications

Evidence of neocortex-like primitive laminar pallium in avian embryo and its organization by microRNA-19b

Evidence of neocortex-like primitive laminar pallium in avian embryo and its organization by microRNA-19b

The role of microRNAs in neurobiology and pathophysiology of the hippocampus

Lmx1a is a master regulator of the cortical hem

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